pCMV-Fluc-SUPT16H-3UTR-Puro pl

pCMV-Fluc-SUPT16H-3UTR-Puro plasmid

PVT96495

pCMV-Fluc-SUPT16H-3UTR-Puro plasmid Information

Plasmid name: pCMV-Fluc-SUPT16H(human)-3UTR-Puro
Alias: -
Gene lenght: 1197bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Luc-F
Reverse primer:
Copy number: High
Induction:

pCMV-Fluc-SUPT16H-3UTR-Puro plasmid Description

pCMV-Fluc-SUPT16H-3UTR-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-Fluc-SUPT16H-3UTR-Puro plasmid Sequence

1 GACGAAAGAT TGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 61 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 121 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT 181 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 241 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG 301 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAGCTTGC ATGCCTGCAG GTCGGCCGCC 361 ACGACCGGTG CCGCCACCAT CCCCTGACCC ACGCCCCTGA CCCCTCACAA GGAGACGACC 421 TTCCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC 481 CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC 541 GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT 601 CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC 661 GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG 721 CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA 781 GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT 841 GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT 901 CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC 961 CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC 1021 CTGACGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGGCTC 1081 CGACCGAAGC CACCCGGGGC GGCCCCGCCG ACCCCGCACC CGCCCCCGAG GCCCACCGAC 1141 TCTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC 1201 TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT 1261 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 1321 CATTTTTTTC ACTGCAATCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA 1381 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 1441 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 1501 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 1561 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 1621 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 1681 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 1741 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 1801 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 1861 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 1921 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 1981 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 2041 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 2101 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 2161 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 2221 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 2281 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 2341 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 2401 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 2461 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 2521 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 2581 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTT TTTGCCGGAT 2641 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 2701 ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 2761 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 2821 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 2881 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 2941 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 3001 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 3061 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 3121 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 3181 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 3241 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 3301 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 3361 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 3421 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 3481 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 3541 AGCTATGACA TGATTACGAA TTGCAACGAT TTAGGTGACA CTATAGAAGA GAAGGAATTA 3601 ATACGACTCA CTATAGGGAG AGAGAGAGAA TTACCCTCAC TAAAGGGAGG AGAAGCATGA 3661 ATTCAAGGTA CCAGATCTTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 3721 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 3781 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 3841 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 3901 TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 3961 CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT 4021 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA 4081 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 4141 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA 4201 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTGGTT TAGTGAACCG 4261 TGGATCCACC ATGGAAGACG CCAAAAACAT AAAGAAAGGC CCGGCGCCAT TCTATCCTCT 4321 AGAGGATGGA ACCGCTGGAG AGCAACTGCA TAAGGCTATG AAGAGATACG CCCTGGTTCC 4381 TGGAACAATT GCTTTTACAG ATGCACATAT CGAGGTGAAC ATCACGTACG CGGAATACTT 4441 CGAAATGTCC GTTCGGTTGG CAGAAGCTAT GAAACGATAT GGGCTGAATA CAAATCACAG 4501 AATCGTCGTA TGCAGTGAAA ACTCTCTTCA ATTCTTTATG CCGGTGTTGG GCGCGTTATT 4561 TATCGGAGTT GCAGTTGCGC CCGCGAACGA CATTTATAAT GAACGTGAAT TGCTCAACAG 4621 TATGAACATT TCGCAGCCTA CCGTAGTGTT TGTTTCCAAA AAGGGGTTGC AAAAAATTTT 4681 GAACGTGCAA AAAAAATTAC CAATAATCCA GAAAATTATT ATCATGGATT CTAAAACGGA 4741 TTACCAGGGA TTTCAGTCGA TGTACACGTT CGTCACATCT CATCTACCTC CCGGTTTTAA 4801 TGAATACGAT TTTGTACCAG AGTCCTTTGA TCGTGACAAA ACAATTGCAC TGATAATGAA 4861 TTCCTCTGGA TCTACTGGGT TACCTAAGGG TGTGGCCCTT CCGCATAGAA CTGCCTGCGT 4921 CAGATTCTCG CATGCCAGAG ATCCTATTTT TGGCAATCAA ATCGTTCCGG ATACTGCGAT 4981 TTTAAGTGTT GTCCCATTCC ATCACGGTTT TGGAATGTTT ACTACACTCG GATATTTGAT 5041 ATGTGGATTT CGAGTCGTCT TAATGTATAG ATTTGAAGAA GAGCTGTTTT TACGATCCCT 5101 TCAGGATTAC AAAATTCAAA GTGCGTTGCT AGTACCAACC CTATTTTCAT TCTTCGCCAA 5161 AAGCACTCTG ATTGACAAAT ACGATTTATC TAATTTACAC GAAATTGCTT CTGGGGGCGC 5221 ACCTCTTTCG AAAGAAGTCG GGGAAGCGGT TGCAAAACGC TTCCATCTTC CAGGGATACG 5281 ACAAGGATAT GGGCTCACTG AGACTACATC AGCTATTCTG ATTACACCCG AGGGGGATGA 5341 TAAACCGGGC GCGGTCGGTA AAGTTGTTCC ATTTTTTGAA GCGAAGGTTG TGGATCTGGA 5401 TACCGGGAAA ACGCTGGGCG TTAATCAGAG AGGCGAATTA TGTGTCAGAG GACCTATGAT 5461 TATGTCCGGT TATGTAAACA ATCCGGAAGC GACCAACGCC TTGATTGACA AGGATGGATG 5521 GCTACATTCT GGAGACATAG CTTACTGGGA CGAAGACGAA CACTTCTTCA TAGTTGACCG 5581 CTTGAAGTCT TTAATTAAAT ACAAAGGATA TCAGGTGGCC CCCGCTGAAT TGGAATCGAT 5641 ATTGTTACAA CACCCCAACA TCTTCGACGC GGGCGTGGCA GGTCTTCCCG ACGATGACGC 5701 CGGTGAACTT CCCGCCGCCG TTGTTGTTTT GGAGCACGGA AAGACGATGA CGGAAAAAGA 5761 GATCGTGGAT TACGTCGCCA GTCAAGTAAC AACCGCGAAA AAGTTGCGCG GAGGAGTTGT 5821 GTTTGTGGAC GAAGTACCGA AAGGTCTTAC CGGAAAACTC GACGCAAGAA AAATCAGAGA 5881 GATCCTCATA AAGGCCAAGA AGGGCGGAAA GTCCAAATTG CTCGAGTGAC TTCTGAACTT 5941 TGGCCCTGAG CTCCATTCTT CCTCCAGCCA ACCCCTGAAA ATTTTACATG ACATAGAAAC 6001 TGTATTTTTC CTTTCGTTTT CATTTGAAGT TTTGCCATTT GTGTTTATGG GTTTAGGGGG 6061 CCATTTGTGT GGACCAATCT ACTCGGGGAA TTCCAGGCCC ACCAGGACAC GTGCCAATGG 6121 CCCCATTCAG ATGGCAAGGG AGGAGGTGTT CTTGAAGACA GGAGGAGGCT CCCGCTGTTA 6181 ATAAATATTG TTTCATTCTT CTCTCTTCCT GTCACCTTCT GCCAAGACAT TGATGGCTTC 6241 TGACATCTTA TTTGGTGTCT CAAAGCTGTA TTTCCAAGAC AGTGGTACAA GGTGACCCTT 6301 AATTACCCGT ATCATGGTTC TTGACCAGCA CATTCAATCC TCCAACCTAC CCTACTGCCA 6361 TGACCTTCCG CACATCTCTA AGTTTTATCT TTGCAATACT CAAGGTTCTC GGAAATTTGC 6421 TAATGGTTGT GATAAACCAT ACAGCTTGAG CCAGTGAGGC AGATTGGGCT GGTGCCTTCG 6481 TCTGAGTTTT CCTGCTTTCC TGCCTCGTGC AGATTCTGAG GTATATCTGC TGCCTTGGAA 6541 GACATAAGAA GCAGTGATAC TCCCTGGCTC GGTTATTTTC TCCATACAAT GCACACATGG 6601 TACAATGATA GAAGGCAAAA TTGCCACTGT CTTCTTTTTT TTCTCATATA TCTAAGGAAG 6661 ATATATCAGG TTGTGCCTCA TGTACCGCTT CTAGTGAAAT GTAGAGGAAG GCTCAAAGGA 6721 GTCAACATTT ATATCTGGAA GGGACAAGTC ATGCCTTGGG CCTAGAATAC CCTGATGAGA 6781 AAAGAGAAGA GGAAGGGAGG CCATATCTAC AACACAGCCT CTCGGCACTG CTGCTCCTTA 6841 TTTTAACTTT GTCTTGCATT GTCCTGTATT TATCACAGTT TCTGTTGAAC AGCTTTTCAA 6901 GTATTTGGGG AGTTTATCTT GCCATCCTCC CCTTCTGGTT CTCTGCACCC ACCTGTCCCA 6961 CTGCAGTTCC TTCCGTGCTC TGTGACTTTA AGAGAAGAAG GGGGGAGGGG TCCCGGATTT 7021 TATGTTTGTT TGTTTTTTCT CCTTAGCAGT AGGACTTGAT ATTTTCAATT TTGGAAGAAC 7081 TAAAAGATGA ATAAACTGGG TTTTTTTTGT TGTTTGTTTT TGTAAAGAGC TCATAGGCCG 7141 GCATAGACGC GTGCGTGATA TCATAGGTTT AAACAGTTAA GCTTAATAAA GGATCTTTTA 7201 TTTTCATTGG ATCTGTGTGT TGGTTTTTTG TATGCGGCCG CTAGCTTGGC ACTGGCCGTC 7261 GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA 7321 CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA 7381 CAGTTGCGCA GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG 7441 TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG 7501 TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC 7561 CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT 7621 TCACCGTCAT CACCGAAACG CGCGA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.