pLV3-CMV-QKI-3×FLAG-CopGFP-Pur

pLV3-CMV-QKI-3×FLAG-CopGFP-Puro plasmid

PVT96492

pLV3-CMV-QKI-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-QKI(human)-3×FLAG-CopGFP-Puro
Alias: NM_006775.3;Hqk; hqkI; QK; QK1; QK3
Gene lenght: 1023bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-QKI-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-QKI-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-QKI-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg tcggggaaat ggaaacgaag 2341 gagaagccga agcccacccc agattacctg atgcagctga tgaacgacaa gaagctcatg 2401 agcagcctgc ccaacttctg cgggatcttc aaccacctcg agcggctgct ggacgaagaa 2461 attagcagag tacggaaaga catgtacaat gacacattaa atggcagtac agagaaaagg 2521 agtgcagaat tgcctgatgc tgtgggacct attgttcagt tacaagagaa actttatgtg 2581 cctgtaaaag aatacccaga ttttaatttt gttgggagaa tccttggacc tagaggactt 2641 acagccaaac aacttgaagc agaaaccgga tgtaaaatca tggtccgagg caaaggctca 2701 atgagggata aaaaaaagga ggagcaaaat agaggcaagc ccaattggga gcatctaaat 2761 gaagatttac atgtactaat cactgtggaa gatgctcaga acagagcaga aatcaaattg 2821 aagagagcag ttgaagaagt gaagaaatta ttggtacctg cagcagaagg agaagacagc 2881 ctgaagaaga tgcagctgat ggagcttgcg attctgaatg gcacctacag agatgccaac 2941 attaaatcac cagcccttgc cttttctctt gcagcaacag cccaggctgc tccaaggatc 3001 attactgggc ctgcgccggt tctcccacca gctgccctgc gtactcctac gccagctggc 3061 cctaccataa tgcctttgat cagacaaata cagaccgctg tcatgccaaa cggaactcct 3121 cacccaactg ctgcaatagt tcctccaggg cccgaagctg gtttaatcta tacaccctat 3181 gagtacccct acacattggc accagctaca tcaatccttg agtatcctat tgaacctagt 3241 ggtgtattag gtgcggtggc tactaaagtt cgaaggcacg atatgcgtgt ccatccttac 3301 caaaggattg tgaccgcaga ccgagccgcc accggcaacg gatccGACTA CAAAGACCAT 3361 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 3421 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 3481 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 3541 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 3601 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 3661 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 3721 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 3781 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 3841 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 3901 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 3961 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 4021 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 4081 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 4141 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 4201 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 4261 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 4321 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 4381 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 4441 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 4501 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 4561 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 4621 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 4681 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 4741 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 4801 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 4861 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 4921 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 4981 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 5041 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 5101 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 5161 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 5221 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 5281 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 5341 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 5401 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5461 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5521 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5581 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5641 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5701 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 5761 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 5821 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 5881 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 5941 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 6001 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 6061 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 6121 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6181 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6241 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6301 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6361 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6421 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6481 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 6541 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6601 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6661 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 6721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 6781 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6841 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7501 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7861 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7921 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7981 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8041 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8101 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8161 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 8221 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8281 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 8341 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8401 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8461 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8521 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8581 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8641 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8701 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 8761 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 8821 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 8881 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 8941 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 9001 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 9061 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 9121 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 9181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 9241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 9301 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.