pLV2-CMV-USP39-Puro plasmid

pLV2-CMV-USP39-Puro plasmid

PVT96461

pLV2-CMV-USP39-Puro plasmid Information

Plasmid name: pLV2-CMV-USP39(human)-Puro
Alias: NM_006590.4;65K; CGI-21; HSPC332; SAD1; SNRNP65
Gene lenght: 1698bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-USP39-Puro plasmid Description

pLV2-CMV-USP39-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-USP39-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgtccgg ccggtctaag cgggagtctc gcggttccac 2881 tcgcgggaag cgagagtctg agtcgcgggg cagctccggt cgcgtcaagc gggagcgaga 2941 tcgggagcgg gagcctgagg cggcgagctc ccggggcagc cctgtgcgcg tgaagcggga 3001 gttcgagccg gcgagcgcgc gcgaggcccc ggcttctgtt gtcccgtttg tgcgggtgaa 3061 gcgggagcgc gaggtcgatg aggactcgga gcctgagcgg gaggtgcgag caaagaatgg 3121 ccgagtggat tctgaggacc ggaggagccg ccactgcccg tacctggaca ccattaacag 3181 gagtgtgctg gactttgact ttgagaaact gtgttctatc tccctctcac acatcaatgc 3241 ttatgcctgt ctggtgtgtg gcaagtactt tcaaggccgg ggtttgaagt ctcacgccta 3301 cattcacagt gtccagttta gccaccatgt tttcctcaac ctccacaccc tcaagtttta 3361 ctgccttcca gacaactatg agatcatcga ttcctcattg gaggatatca cgtatgtgtt 3421 gaagcccact ttcacaaagc agcaaattgc aaacttggac aagcaagcca aattgtcccg 3481 ggcatatgat ggtaccactt acctgccggg tattgtggga ctgaataaca taaaggccaa 3541 tgattatgcc aacgctgtcc ttcaggctct atctaatgtt cctcctctcc ggaactactt 3601 tctggaagaa gacaattata agaacatcaa acgtcctcca ggggatatca tgttcttgtt 3661 ggtccagcgt tttggagagc tgatgagaaa gctctggaac cctcgaaatt tcaaggcaca 3721 tgtgtctccc catgagatgc ttcaggcagt tgtactttgc agtaagaaga cttttcagat 3781 caccaaacaa ggagatggcg ttgactttct gtcttggttt ctgaatgctc tgcactcagc 3841 tctggggggc acaaagaaga aaaagaagac tattgtgact gatgttttcc aggggtccat 3901 gaggatcttc actaaaaagc ttccccatcc tgatctgcca gcagaagaaa aagagcagtt 3961 gctccataat gacgagtacc aggagacaat ggtggagtcc acttttatgt acctgacgct 4021 ggaccttcct actgcccccc tctacaagga cgagaaggag cagctcatca ttccccaagt 4081 gccactcttc aacatcctgg ctaagttcaa tggcatcact gagaaggaat ataagactta 4141 caaggagaac tttctgaagc gcttccagct taccaagttg cctccatatc taatcttttg 4201 tatcaagaga ttcactaaga acaacttctt tgttgagaag aatccaacta ttgtcaattt 4261 ccctattaca aatgtggatc tgagagaata cttgtctgaa gaagtacaag cagtacacaa 4321 gaataccacc tatgacctca ttgccaacat cgtgcatgac ggcaagccct ccgagggctc 4381 ctaccggatc cacgtgcttc atcatgggac aggcaaatgg tatgaattac aagacctcca 4441 ggtgactgac atccttcccc agatgatcac actgtcagag gcttacattc agatttggaa 4501 gaggcgagat aatgatgaaa ccaaccagca gggggcttga ggatcccgcg actctagata 4561 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 4621 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 4681 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 4741 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 4801 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 4861 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 4921 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 4981 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 5041 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 5101 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5161 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 5221 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 5281 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5341 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5401 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5461 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 5521 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 5581 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 5641 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 5701 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5761 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5821 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 5881 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 5941 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6001 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6061 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 6121 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6181 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6241 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 6301 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 6361 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 6421 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6481 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 6541 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 6601 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 6661 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 6721 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 6781 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 6841 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 6901 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 6961 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 7021 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 7081 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 7141 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 7201 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 7261 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7321 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7381 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7441 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7501 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7561 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7621 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7681 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7741 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7801 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7861 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7921 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 7981 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 8041 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 8101 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8161 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 8221 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8281 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8341 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8401 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8461 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8521 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8581 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8641 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8701 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8761 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8821 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8881 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8941 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 9001 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 9061 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9121 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9181 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9241 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9301 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9361 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 9421 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 9481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 9541 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 9601 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 9661 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 9721 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 9781 t

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.