pLV3-CMV-HAND2-EF1a-CopGFP-Pur

pLV3-CMV-HAND2-EF1a-CopGFP-Puro plasmid

PVT96443

pLV3-CMV-HAND2-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HAND2(human)-EF1a-CopGFP-Puro
Alias: NM_021973.3;bHLHa26; dHand; DHAND2; Hed; Thing2
Gene lenght: 654bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HAND2-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-HAND2-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HAND2-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgagtct ggtaggtggt tttccccacc acccggtggt 2341 gcaccacgag ggctacccgt ttgccgccgc cgccgccgca gctgccgccg ccgccgccag 2401 ccgctgcagc catgaggaga acccctactt ccatggctgg ctcatcggcc accccgagat 2461 gtcgcccccc gactacagca tggccctgtc ctacagcccc gagtatgcca gcggcgccgc 2521 cggcctggac cactcccatt acgggggggt gccgccgggc gccgggcccc cgggcctggg 2581 ggggccgcgc ccggtgaagc gccgaggcac cgccaaccgc aaggagcggc gcaggactca 2641 gagcatcaac agcgccttcg ccgaactgcg cgagtgcatc cccaacgtac ccgccgacac 2701 caaactctcc aaaatcaaga ccctgcgcct ggccaccagc tacatcgcct acctcatgga 2761 cctgctggcc aaggacgacc agaatggcga ggcggaggcc ttcaaggcag agatcaagaa 2821 gaccgacgtg aaagaggaga agaggaagaa ggagctgaac gaaatcttga aaagcacagt 2881 gagcagcaac gacaagaaaa ccaaaggccg gacgggctgg ccgcagcacg tctgggccct 2941 ggagctcaag cagtgaggat ccgcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc 3001 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt 3061 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc 3121 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg 3181 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct 3241 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc 3301 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc 3361 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc 3421 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc 3481 tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg 3541 acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg 3601 tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca 3661 agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg 3721 gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg 3781 ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc 3841 tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg 3901 tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg 3961 ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca 4021 ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca 4081 agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg 4141 tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga 4201 cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg 4261 ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg 4321 tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg 4381 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 4441 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 4501 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg 4561 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 4621 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 4681 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc 4741 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 4801 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 4861 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga 4921 cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg 4981 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 5041 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 5101 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 5161 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 5221 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 5281 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 5341 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 5401 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 5461 tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc 5521 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 5581 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc 5641 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 5701 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 5761 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 5821 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 5881 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat 5941 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 6001 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac 6061 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 6121 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 6181 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg 6241 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 6301 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 6361 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 6421 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 6601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 6661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 6721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 6781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 6841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 6901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 6961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7021 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 7621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 7681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 7741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 7801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 7861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 7921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 7981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 8161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 8221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 8281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 8341 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 8401 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 8461 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 8521 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 8581 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 8641 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 8701 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 8761 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 8821 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.