pLV3-CMV-HNRNPA2B1-3×FLAG-CopG

pLV3-CMV-HNRNPA2B1-3×FLAG-CopGFP-Hyg plasmid

PVT96120

pLV3-CMV-HNRNPA2B1-3×FLAG-CopGFP-Hyg plasmid Information

Plasmid name: pLV3-CMV-HNRNPA2B1(human)-3×FLAG-CopGFP-Hyg
Alias: NM_031243.3;HNRNPA2; HNRNPB1; HNRPA2; HNRPA2B1; HNRPB1;IBMPFD2; OPMD2; RNPA2; SNRPB1
Gene lenght: 1059bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-HNRNPA2B1-3×FLAG-CopGFP-Hyg plasmid Description

pLV3-CMV-HNRNPA2B1-3×FLAG-CopGFP-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HNRNPA2B1-3×FLAG-CopGFP-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagatct 2281 cgaGCCACCa tggagaaaac tttagaaact gttcctttgg agaggaaaaa gagagaaaag 2341 gaacagttcc gtaagctctt tattggtggc ttaagctttg aaaccacaga agaaagtttg 2401 aggaactact acgaacaatg gggaaagctt acagactgtg tggtaatgag ggatcctgca 2461 agcaaaagat caagaggatt tggttttgta actttttcat ccatggctga ggttgatgct 2521 gccatggctg caagacctca ttcaattgat gggagagtag ttgagccaaa acgtgctgta 2581 gcaagagagg aatctggaaa accaggggct catgtaactg tgaagaagct gtttgttggc 2641 ggaattaaag aagatactga ggaacatcac cttagagatt actttgagga atatggaaaa 2701 attgatacca ttgagataat tactgatagg cagtctggaa agaaaagagg ctttggcttt 2761 gttacttttg atgaccatga tcctgtggat aaaatcgtat tgcagaaata ccataccatc 2821 aatggtcata atgcagaagt aagaaaggct ttgtctagac aagaaatgca ggaagttcag 2881 agttctagga gtggaagagg aggcaacttt ggctttgggg attcacgtgg tggcggtgga 2941 aatttcggac caggaccagg aagtaacttt agaggaggat ctgatggata tggcagtgga 3001 cgtggatttg gggatggcta taatgggtat ggaggaggac ctggaggtgg caattttgga 3061 ggtagccccg gttatggagg aggaagagga ggatatggtg gtggaggacc tggatatggc 3121 aaccagggtg ggggctacgg aggtggttat gacaactatg gaggaggaaa ttatggaagt 3181 ggaaattaca atgattttgg aaattataac cagcaacctt ctaactacgg tccaatgaag 3241 agtggaaact ttggtggtag caggaacatg gggggaccat atggtggagg aaactatggt 3301 ccaggaggca gtggaggaag tgggggttat ggtgggagga gccgatacgg atccGACTAC 3361 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 3421 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3481 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 3541 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 3601 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 3661 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 3721 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 3781 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 3841 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 3901 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 3961 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4021 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4081 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4141 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4201 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4261 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4321 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4381 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 4441 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 4501 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 4561 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 4621 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 4681 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 4741 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 4801 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac 4861 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat 4921 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat 4981 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt 5041 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg 5101 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat 5161 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga 5221 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat 5281 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag 5341 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc 5401 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg 5461 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct 5521 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg 5581 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac 5641 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga 5701 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc 5761 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag 5821 gaataggtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5881 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5941 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 6001 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 6061 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 6121 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 6181 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt 6241 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 6301 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 6361 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 6421 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 6481 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt 6541 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 6601 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 6661 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 6721 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 6781 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata 6841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 6901 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag 6961 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 7021 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt 7081 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 7141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 7201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 7261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 7321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 7381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7921 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 8041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 8101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 8161 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 8221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 8281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 8341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 8401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 9001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 9061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 9121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 9181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 9241 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 9301 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 9361 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 9421 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 9481 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 9541 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 9601 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 9661 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 9721 ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.