pLV3-CMV-GPR61-GPR78-CopGFP-Pu

pLV3-CMV-GPR61-GPR78-CopGFP-Puro plasmid

PVT96392

pLV3-CMV-GPR61-GPR78-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GPR61(human)-GPR78(human)-CopGFP-Puro
Alias: NM_031936.6BALGR; GPCR3/NM_080819.5;NM_014481.4;APE2; APEXL2; XTH2; ZGRF2
Gene lenght: 1353/1557bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GPR61-GPR78-CopGFP-Puro plasmid Description

pLV3-CMV-GPR61-GPR78-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-GPR61-GPR78-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agtcctcacc catcccccag 2341 tcatcaggga actcttccac tttggggagg gtccctcaaa ccccaggtcc ctctactgcc 2401 agtggggtcc cggaggtggg gctacgggat gttgcttcgg aatctgtggc cctcttcttc 2461 atgctcctgc tggacttgac tgctgtggct ggcaatgccg ctgtgatggc cgtgatcgcc 2521 aagacgcctg ccctccgaaa atttgtcttc gtcttccacc tctgcctggt ggacctgctg 2581 gctgccctga ccctcatgcc cctggccatg ctctccagct ctgccctctt tgaccacgcc 2641 ctctttgggg aggtggcctg ccgcctctac ttgtttctga gcgtgtgctt tgtcagcctg 2701 gccatcctct cggtgtcagc catcaatgtg gagcgctact attacgtagt ccaccccatg 2761 cgctacgagg tgcgcatgac gctggggctg gtggcctctg tgctggtggg tgtgtgggtg 2821 aaggccttgg ccatggcttc tgtgccagtg ttgggaaggg tctcctggga ggaaggagct 2881 cccagtgtcc ccccaggctg ttcactccag tggagccaca gtgcctactg ccagcttttt 2941 gtggtggtct ttgctgtcct ttactttctg ttgcccctgc tcctcatact tgtggtctac 3001 tgcagcatgt tccgagtggc ccgcgtggct gccatgcagc acgggccgct gcccacgtgg 3061 atggagacac cccggcaacg ctccgaatct ctcagcagcc gctccacgat ggtcaccagc 3121 tcgggggccc cccagaccac cccacaccgg acgtttgggg gagggaaagc agcagtggtt 3181 ctcctggctg tggggggaca gttcctgctc tgttggttgc cctacttctc tttccacctc 3241 tatgttgccc tgagtgctca gcccatttca actgggcagg tggagagtgt ggtcacctgg 3301 attggctact tttgcttcac ttccaaccct ttcttctatg gatgtctcaa ccggcagatc 3361 cggggggagc tcagcaagca gtttgtctgc ttcttcaagc cagctccaga ggaggagctg 3421 aggctgccta gccgggaggg ctccattgag gagaacttcc tgcagttcct tcaggggact 3481 ggctgtcctt ctgagtcctg ggtttcccga cccctaccca gccccaagca ggagccacct 3541 gctgttgact ttcgaatccc aggccagata gctgaggaga cctctgagtt cctggagcag 3601 caactcacca gcgacatcat catgtcagac agctacctcc gtcctgccgc ctcaccccgg 3661 ctggagtcag gtggaggcgg atcaggaggc ggtggatcta tgttgcgcgt ggtgagctgg 3721 aacatcaatg ggattcggag acccctgcaa ggggtggcaa atcaggaacc cagcaactgt 3781 gccgccgtgg ccgtggggcg cattttggac gagctggatg cggatatcgt ctgtctccag 3841 gaaaccaaag tgaccaggga tgcactgaca gagcccctgg ctatcgttga gggttataac 3901 tcctatttca gcttcagccg caaccgtagc ggctattctg gtgtagccac cttctgtaag 3961 gacaatgcta ccccagtggc tgctgaagaa ggcctgagtg gcctgtttgc cacccagaat 4021 ggggatgttg gttgctatgg aaacatggat gagtttaccc aagaggaact ccgggctctg 4081 gatagtgagg gcagggccct cctcacacag cataagatcc gcacatggga aggtaaggag 4141 aagaccttga ccctaatcaa cgtgtactgc ccccatgcgg accctgggag gcctgagcgg 4201 ctagtcttta agatgcgctt ctatcgtttg ctgcaaatcc gagcagaagc cctcctggcg 4261 gcaggcagcc atgtgatcat tctgggtgac ctgaatacag cccaccgccc cattgaccac 4321 tgggatgcag tcaacctgga atgctttgaa gaggacccag ggcgcaagtg gatggacagc 4381 ttgctcagta acttggggtg ccagtctgcc tctcatgtag ggcccttcat cgatagctac 4441 cgctgcttcc aaccaaagca ggagggggcc ttcacctgct ggtcagcagt cactggcgcc 4501 cgccatctca actatggctc ccggcttgac tatgtgctgg gggacaggac cctggtcata 4561 gacacctttc aggcctcttt cctgctgcct gaggtgatgg gctctgacca ctgccctgtg 4621 ggtgcagtct tgagtgtgtc ctctgtgcct gcaaaacagt gcccacctct gtgcacccgc 4681 ttcctccctg agtttgcagg cacccagctc aagatccttc gcttcctagt tcctctcgaa 4741 caaagtcctg tgttggagca gtcgacgctg cagcacaaca atcaaacccg ggtacagaca 4801 tgccaaaaca aagcccaagt gcgctcaacc aggcctcagc ccagtcaggt tggctctagc 4861 agaggccaga aaaacctgaa gagctacttt cagccctccc ctagctgtcc ccaagcctct 4921 cctgacatag agctgcctag cctaccactg atgagcgccc tcatgacccc gaagactcca 4981 gaagagaagg cagtggccaa agtggtgaag gggcaggcca agacttcaga agccaaagat 5041 gagaaggagt tacggacctc attctggaag tctgtgctgg cggggccctt gcgcacaccc 5101 ctctgtgggg gccacaggga gccatgtgtg atgcgtactg tgaagaagcc aggacccaac 5161 ttgggccgcc gcttctacat gtgtgccagg ccccggggtc ctcccactga cccctcctcc 5221 cggtgcaact tcttcctctg gagcaggccc agctgaggat ccCTGCGATA TCCAGCgcgg 5281 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 5341 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 5401 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 5461 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 5521 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 5581 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 5641 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 5701 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 5761 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 5821 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 5881 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 5941 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 6001 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 6061 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 6121 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 6181 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 6241 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 6301 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 6361 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 6421 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 6481 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 6541 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 6601 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 6661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 6721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 6781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 6841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 6901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 6961 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 7021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 7081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 7141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 7201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 7261 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 7321 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 7381 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 7441 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 7501 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 7561 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 7621 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 7681 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 7741 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt 7801 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca 7861 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 7921 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg 7981 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 8041 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 8101 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 8161 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 8221 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 8281 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 8341 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc 8401 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 8461 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa 8521 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 8581 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 8641 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 8701 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 8761 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 8821 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 8881 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 8941 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 9001 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 9061 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 9121 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 9181 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 9241 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 9301 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 9361 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 9421 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 9481 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 9541 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 9601 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 9661 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 9721 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 9781 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 9841 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 9901 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 9961 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 10021 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 10081 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 10141 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 10201 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 10261 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 10321 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 10381 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 10441 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 10501 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 10561 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 10621 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 10681 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 10741 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 10801 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 10861 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 10921 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 10981 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 11041 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 11101 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.