pLV3-CMV-FLAG-GPR61-GPR78-CopG

pLV3-CMV-FLAG-GPR61-GPR78-CopGFP-Puro plasmid

PVT96355

pLV3-CMV-FLAG-GPR61-GPR78-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FLAG-GPR61(human)-GPR78(human)-CopGFP-Puro
Alias: NM_031936.6BALGR; GPCR3/NM_080819.5;NM_014481.4;APE2; APEXL2; XTH2; ZGRF2
Gene lenght: 1353/1557bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FLAG-GPR61-GPR78-CopGFP-Puro plasmid Description

pLV3-CMV-FLAG-GPR61-GPR78-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-FLAG-GPR61-GPR78-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGG ACTACAAGGA CGACGATGAC 2341 AAGatggagt cctcacccat cccccagtca tcagggaact cttccacttt ggggagggtc 2401 cctcaaaccc caggtccctc tactgccagt ggggtcccgg aggtggggct acgggatgtt 2461 gcttcggaat ctgtggccct cttcttcatg ctcctgctgg acttgactgc tgtggctggc 2521 aatgccgctg tgatggccgt gatcgccaag acgcctgccc tccgaaaatt tgtcttcgtc 2581 ttccacctct gcctggtgga cctgctggct gccctgaccc tcatgcccct ggccatgctc 2641 tccagctctg ccctctttga ccacgccctc tttggggagg tggcctgccg cctctacttg 2701 tttctgagcg tgtgctttgt cagcctggcc atcctctcgg tgtcagccat caatgtggag 2761 cgctactatt acgtagtcca ccccatgcgc tacgaggtgc gcatgacgct ggggctggtg 2821 gcctctgtgc tggtgggtgt gtgggtgaag gccttggcca tggcttctgt gccagtgttg 2881 ggaagggtct cctgggagga aggagctccc agtgtccccc caggctgttc actccagtgg 2941 agccacagtg cctactgcca gctttttgtg gtggtctttg ctgtccttta ctttctgttg 3001 cccctgctcc tcatacttgt ggtctactgc agcatgttcc gagtggcccg cgtggctgcc 3061 atgcagcacg ggccgctgcc cacgtggatg gagacacccc ggcaacgctc cgaatctctc 3121 agcagccgct ccacgatggt caccagctcg ggggcccccc agaccacccc acaccggacg 3181 tttgggggag ggaaagcagc agtggttctc ctggctgtgg ggggacagtt cctgctctgt 3241 tggttgccct acttctcttt ccacctctat gttgccctga gtgctcagcc catttcaact 3301 gggcaggtgg agagtgtggt cacctggatt ggctactttt gcttcacttc caaccctttc 3361 ttctatggat gtctcaaccg gcagatccgg ggggagctca gcaagcagtt tgtctgcttc 3421 ttcaagccag ctccagagga ggagctgagg ctgcctagcc gggagggctc cattgaggag 3481 aacttcctgc agttccttca ggggactggc tgtccttctg agtcctgggt ttcccgaccc 3541 ctacccagcc ccaagcagga gccacctgct gttgactttc gaatcccagg ccagatagct 3601 gaggagacct ctgagttcct ggagcagcaa ctcaccagcg acatcatcat gtcagacagc 3661 tacctccgtc ctgccgcctc accccggctg gagtcaggtg gaggcggatc aggCggTggc 3721 ggatcaatgt tgcgcgtggt gagctggaac atcaatggga ttcggagacc cctgcaaggg 3781 gtggcaaatc aggaacccag caactgtgcc gccgtggccg tggggcgcat tttggacgag 3841 ctggatgcgg atatcgtctg tctccaggaa accaaagtga ccagggatgc actgacagag 3901 cccctggcta tcgttgaggg ttataactcc tatttcagct tcagccgcaa ccgtagcggc 3961 tattctggtg tagccacctt ctgtaaggac aatgctaccc cagtggctgc tgaagaaggc 4021 ctgagtggcc tgtttgccac ccagaatggg gatgttggtt gctatggaaa catggatgag 4081 tttacccaag aggaactccg ggctctggat agtgagggca gggccctcct cacacagcat 4141 aagatccgca catgggaagg taaggagaag accttgaccc taatcaacgt gtactgcccc 4201 catgcggacc ctgggaggcc tgagcggcta gtctttaaga tgcgcttcta tcgtttgctg 4261 caaatccgag cagaagccct cctggcggca ggcagccatg tgatcattct gggtgacctg 4321 aatacagccc accgccccat tgaccactgg gatgcagtca acctggaatg ctttgaagag 4381 gacccagggc gcaagtggat ggacagcttg ctcagtaact tggggtgcca gtctgcctct 4441 catgtagggc ccttcatcga tagctaccgc tgcttccaac caaagcagga gggggccttc 4501 acctgctggt cagcagtcac tggcgcccgc catctcaact atggctcccg gcttgactat 4561 gtgctggggg acaggaccct ggtcatagac acctttcagg cctctttcct gctgcctgag 4621 gtgatgggct ctgaccactg ccctgtgggt gcagtcttga gtgtgtcctc tgtgcctgca 4681 aaacagtgcc cacctctgtg cacccgcttc ctccctgagt ttgcaggcac ccagctcaag 4741 atccttcgct tcctagttcc tctcgaacaa agtcctgtgt tggagcagtc gacgctgcag 4801 cacaacaatc aaacccgggt acagacatgc caaaacaaag cccaagtgcg ctcaaccagg 4861 cctcagccca gtcaggttgg ctctagcaga ggccagaaaa acctgaagag ctactttcag 4921 ccctccccta gctgtcccca agcctctcct gacatagagc tgcctagcct accactgatg 4981 agcgccctca tgaccccgaa gactccagaa gagaaggcag tggccaaagt ggtgaagggg 5041 caggccaaga cttcagaagc caaagatgag aaggagttac ggacctcatt ctggaagtct 5101 gtgctggcgg ggcccttgcg cacacccctc tgtgggggcc acagggagcc atgtgtgatg 5161 cgtactgtga agaagccagg acccaacttg ggccgccgct tctacatgtg tgccaggccc 5221 cggggtcctc ccactgaccc ctcctcccgg tgcaacttct tcctctggag caggcccagc 5281 tgaggatccC TGCGATATCC AGCgcggccg cGaaggatct gcgatcgctc cggtgcccgt 5341 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 5401 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 5461 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 5521 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 5581 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 5641 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 5701 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 5761 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 5821 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc 5881 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 5941 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 6001 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 6061 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 6121 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 6181 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 6241 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 6301 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 6361 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 6421 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 6481 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 6541 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 6601 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 6661 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 6721 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 6781 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 6841 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 6901 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 6961 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 7021 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 7081 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 7141 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 7201 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 7261 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact 7321 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 7381 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 7441 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 7501 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 7561 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 7621 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg 7681 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 7741 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 7801 ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg 7861 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 7921 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg 7981 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 8041 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 8101 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 8161 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 8221 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 8281 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 8341 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 8401 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 8461 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 8521 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt 8581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 8641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 8701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 8761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 8821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 8881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 8941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 9001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 9061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 9121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 9181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 9241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 9301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 9361 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 9421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 9481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 9541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 9601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 9661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 9721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 9781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 9841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 9901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 9961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 10021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 10081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 10141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 10201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 10261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 10321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 10381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 10441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 10501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 10561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 10621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 10681 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 10741 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 10801 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 10861 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 10921 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 10981 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc 11041 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 11101 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 11161 gtaaaacgac ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.