pCMV-Lucy-3×HA-rho-OR51E2-3×HA

pCMV-Lucy-3×HA-rho-OR51E2-3×HA-EGFP-Neo plasmid

PVT96378

pCMV-Lucy-3×HA-rho-OR51E2-3×HA-EGFP-Neo plasmid Information

Plasmid name: pCMV-Lucy-3×HA-rho-OR51E2(human)-3×HA-EGFP-Neo
Alias: NM_030774.4;HPRAJ; OR51E3P; OR52A2; PSGR;
Gene lenght: 960bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Lucy-3×HA-rho-OR51E2-3×HA-EGFP-Neo plasmid Description

pCMV-Lucy-3×HA-rho-OR51E2-3×HA-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Lucy-3×HA-rho-OR51E2-3×HA-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCATGA GACCGCAGAT CCTGCTGCTC CTTGCACTCC 961 TTACCCTCGG ACTCGCTTAC CCATACGACG TACCTGATTA TGCAACTTAT CCTTATGATG 1021 TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCAACCGAG ACCTCCCAAG 1081 TGGCTCCAGC Cggtaccatg agttcctgca acttcacaca tgccaccttt gtgcttattg 1141 gtatcccagg attagagaaa gcccatttct gggttggctt ccccctcctt tccatgtatg 1201 tagtggcaat gtttggaaac tgcatcgtgg tcttcatcgt aaggacggaa cgcagcctgc 1261 acgctccgat gtacctcttt ctctgcatgc ttgcagccat tgacctggcc ttatccacat 1321 ccaccatgcc taagatcctt gcccttttct ggtttgattc ccgagagatt agctttgagg 1381 cctgtcttac ccagatgttc tttattcatg ccctctcagc cattgaatcc accatcctgc 1441 tggccatggc ctttgaccgt tatgtggcca tctgccaccc actgcgccat gctgcagtgc 1501 tcaacaatac agtaacagcc cagattggca tcgtggctgt ggtccgcgga tccctctttt 1561 ttttcccact gcctctgctg atcaagcggc tggccttctg ccactccaat gtcctctcgc 1621 actcctattg tgtccaccag gatgtaatga agttggccta tgcagacact ttgcccaatg 1681 tggtatatgg tcttactgcc attctgctgg tcatgggcgt ggacgtaatg ttcatctcct 1741 tgtcctattt tctgataata cgaacggttc tgcaactgcc ttccaagtca gagcgggcca 1801 aggcctttgg aacctgtgtg tcacacattg gtgtggtact cgccttctat gtgccactta 1861 ttggcctctc agtggtacac cgctttggaa acagccttca tcccattgtg cgtgttgtca 1921 tgggtgacat ctacctgctg ctgcctcctg tcatcaatcc catcatctat ggtgccaaaa 1981 ccaaacagat cagaacacgg gtgctggcta tgttcaagat cagctgtgac aaggacttgc 2041 aggctgtggg aggcaagCTC GAGTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT 2101 ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA tactcgagtg 2161 gcggtggagg aagcggcgga ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg 2221 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 2281 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 2341 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt 2401 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 2461 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 2521 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 2581 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 2641 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 2701 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 2761 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 2821 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 2881 ctctcggcat ggacgagctg tacaagtagt ctagagggcc cgtttaaacc cgctgatcag 2941 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 3001 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 3061 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 3121 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 3181 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 3241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 3301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 3361 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 3421 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 3481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 3541 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 3601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 3901 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 3961 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 4021 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 4081 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 4141 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 4201 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 4261 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 4321 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 4381 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 4441 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 4501 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 4561 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 4621 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 4681 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 4741 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 4801 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 4861 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 4921 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 4981 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 5041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 5101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 5161 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 5221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 5281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 5341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 5401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 5461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 5521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 5581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 5641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 5701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 5761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 5821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 5881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 5941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 6001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 6061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 6121 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 6181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 6241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 6301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 6361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 6421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 6481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 6541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 6601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 6661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 6721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 6781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 6841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 6901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 6961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 7021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 7081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 7141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 7201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 7261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 7321 tttccccgaa aagtgccacc tgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.