pLV2-Emilin1-3×FLAG-T2A-AcGFP1

pLV2-Emilin1-3×FLAG-T2A-AcGFP1-Puro plasmid

PVT96167

pLV2-Emilin1-3×FLAG-T2A-AcGFP1-Puro plasmid Information

Plasmid name: pLV2-Emilin1(mouse)-3×FLAG-T2A-AcGFP1-Puro
Alias: NM_133918.2;5830419M17Rik; EMILIN-1; gp115
Gene lenght: 3051bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-Emilin1-3×FLAG-T2A-AcGFP1-Puro plasmid Description

pLV2-Emilin1-3×FLAG-T2A-AcGFP1-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-Emilin1-3×FLAG-T2A-AcGFP1-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggcccc cagagccctc tggagctgct atctctgctg 2881 tctgctgacc atagccacgg aagctgccag ctaccctcct cgaggttaca gcctctacac 2941 aggaggcact ggggccctga gtcctggggg accccaggcc cagaactcac cacggcctgc 3001 cagccgccac aggaactggt gtgcctacgt ggtgactcgg acagtgagct gcgtccttga 3061 ggatggagtg gagaccattg tcaagccaga ctatcaaccc tgtggctggg gccagcccca 3121 ctgttcccga agtatcatgt accggagctt tcttcgccct cgctatcggg tggcttacaa 3181 gacagtgaca gacatggagt ggaggtgctg tcagggttat gggggcgatg actgtggaga 3241 gggccctgct tccgttctgg gcccagcacc ctccacacca cttccccggc ccaggcctgt 3301 gcgccccaac ctgtctggct ccagtgccgg cagccatctc agtggactgg gaggagaagg 3361 tcctgttgag tcagagaagg tgcagcagct agagcggcag gtgaagagtc tgaccaaaga 3421 gctgcaaggc cttcggggtg tcctgcaggg gatgaatggg cgcctagcag aagatgtaca 3481 gcgagctgtg gacacagtct ttaatggaag gcagcagcca gcagatgcag ctgcccgccc 3541 gggcgtgcat gaaaccctca gtgagatcca gcagcagctg cagctcctgg acaaccgtgt 3601 ctccactcat gaccaggagc tgggccacct taacaaccat cataatggag gccctggtgg 3661 aggtggcagg gcctcaggcc ctgtcccagt tccttctggc cccagtgaag aactgttaag 3721 gcagctggaa cggcagctgc aagagtcttg ctcagtgtgc ctgacggggc tggatggctt 3781 ccgccagcag cagcaagagg atagggagcg gctgcgaacc ctggagaagc taatgtcctc 3841 tatggaggag cggcagcaac agcttgtggg acctgccatg gccaggagac cccctcagga 3901 atgctgcccc ccagagctgg gtcgacgagt gtctgagctg gagcggaggc tagatgtagt 3961 gactggctca ctgacagtgc taagtggacg cagaggttct gagcttggag gagcagctgg 4021 gcaggggggc caccctccag gctacaccag cttggcctcc cgcctttctc gcctggagga 4081 ccgcttcaac tctaccctag gtccctcaga ggagcaagag aagaactggc ctggaggacc 4141 agggaggctg ggccactggt tgcctgctgc tccaggacgg ctagaaaagc tggagggact 4201 actagccaat gtgagcaggg agctgggtgg ccgcatggat ctgctggaag agcaggtggc 4261 aggggctgtt cggacttgtg ggcagatttg ctctggggca cccggggaac aggattctcg 4321 ggtcaatgag atcctcagtg ccttggaacg cagggtgctg gacagcgagg gccggttaca 4381 gctagtgggc tctggcttgc atgaagcaga ggcagcaggg gaggctcagc aggccgtgtt 4441 ggagggactg caagggctcc tgagccggct tcgggagcgc atggatgcac aggaggagac 4501 tgcagcagaa atcttactgc gcctcaatct taccgcagcc cagctaagcc agctggaggg 4561 tctgctgcaa gcccgtgggg atgagggttg tggcgcctgt ggtggtgtcc aggaggagct 4621 gggccgcctt cgggatggtg tggaacgttg ctcctgccca ttgttacctc cacggggccc 4681 tggagctggc ccaggggttg ggggaccaag ccgtgggcct ctggatggtt tcagtgtgtt 4741 tgggggcagt tcaggctcag ccctccaggc ccttcaagga gaactctctg aggttattct 4801 caccttcagc tccctgaacg actcactcca cgagctccag accactgtgg agggtcaggg 4861 tgccgatctg gctgacctgg gggccaccaa ggacagcatc atctctgaaa tcaacagact 4921 acagcaggag gccacggagc acgtcacaga gagcgaggag cgcttccgag gcctggagga 4981 gggccaggca caggctggcc aatgccctag cttagagggg cgattaggcc gccttgaggg 5041 agtctgcgaa cgactggaca ccgtggcagg gggactacaa ggcctacgtg aaggcctctc 5101 cagacacgtg gctgggctct gggctgcagt acgggaaagc aacagcacca gcctgacaca 5161 ggccgccctg ctggagaagc tgctgggggg ccaggcaggc ctgggcaggc ggcttggtgc 5221 cctcaacaat tccctgctgc tcctggaaga ccgtctgcag caacttagcc taaaggactt 5281 cactggacct tcaggcaagg ctgggccccc agggcctccc gggctacaag ggccttcagg 5341 ccctgcagga cctccaggac ctcctggcaa agacggacaa cagggggcca ttggcccacc 5401 aggtcctcaa ggggagcagg gagcggaggg agcaccagca gcccctgtgc ctagggtagc 5461 attttcagct gccctgagtt tgccacggtc agaacctggc acagtcccct tcgacagagt 5521 cttgctcaat gatggaggct actatgaccc agagacaggt gtattcactg caccactggc 5581 tggacgctat ttgctgagcg ctgtacttac tgggcaccgg catgagaaag tggaagcagt 5641 attgtcacgc tccaacctgg gcgtggcccg catagactcg ggaggctatg aacccgaggg 5701 actggagaat aagcctgtgg ccgaaagtca gcccagccca ggcgctctgg gcgtcttcag 5761 cctcattctg ccactgcagg tcggagacac tgtctgcatc gacctggtca tggggcagct 5821 ggcacactcc gaggagccgc tcaccatctt cagcggagcc ctgctctacg aggacacaga 5881 gcttgaacag gtgCTCGAGG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT 5941 CGACTACAAG GATGACGATG ACAAGgaggg cagaggaagt cttctaacat gcggtgacgt 6001 ggaggagaat cccggcccta tggtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc 6061 catcctgatc gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg 6121 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 6181 gcctgtgccc tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg 6241 ctaccccgat cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat 6301 ccaggagcgc accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa 6361 gttcgagggc gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga 6421 tggcaacatc ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat 6481 gaccgacaag gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga 6541 tggcagcgtg cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt 6601 gctgctgccc gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga 6661 gaagcgcgat cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat 6721 ggatgagctg tacaagtgag cggccgcgac tctagataat tctaccgggt aggggaggcg 6781 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac 6841 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg 6901 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc 6961 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc 7021 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 7081 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 7141 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 7201 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 7261 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca 7321 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 7381 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 7441 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 7501 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 7561 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 7621 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 7681 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 7741 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 7801 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 7861 gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct ggattacaaa 7921 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 7981 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 8041 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 8101 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 8161 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 8221 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 8281 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 8341 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 8401 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 8461 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa 8521 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag 8581 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa 8641 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag 8701 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag 8761 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct 8821 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag 8881 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag 8941 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc 9001 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg 9061 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca 9121 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg 9181 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 9241 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 9301 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 9361 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt 9421 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 9481 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 9541 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 9601 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 9661 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 9721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 9781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 9841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 9901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 9961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 10021 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 10081 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 10141 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 10201 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 10261 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 10321 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 10381 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 10441 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 10501 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 10561 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 10621 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 10681 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 10741 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 10801 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 10861 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 10921 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 10981 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 11041 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 11101 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 11161 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 11221 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 11281 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 11341 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 11401 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 11461 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 11521 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 11581 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta 11641 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 11701 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 11761 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta 11821 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat 11881 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.