pESR1-enhancer-Fluc-SV40-hRluc

pESR1-enhancer-Fluc-SV40-hRluc-Neo plasmid

PVT96375

pESR1-enhancer-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pESR1(human)-enhancer-Fluc-SV40-hRluc-Neo
Alias: -
Gene lenght: 1101bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc,Rluc
Promoter: ESR1
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: cer region-F1
Reverse primer:
Copy number: High
Induction:

pESR1-enhancer-Fluc-SV40-hRluc-Neo plasmid Description

pESR1-enhancer-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pESR1-enhancer-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattcA CTCCTGACTT AAGCTTACAG CTGATGGGAA ACTTGGGAAC TGGACAGAGA 5161 ATTAAAATAC AACGTGGTTA GGTACAACTT ACCTGCGCCA GAGGAGTGAC GGAAGGCCTT 5221 GGACGGGGAG TGATGCCTGG AGGATGACAG GGCGACAGGT AAGTTAGGGG GCTGGGAAGG 5281 GGAAGGGCGG CCCTGTGGGT AAAGGGAAGG AGGTTAAAGT CAGCAGGGAA ACAGGCAGTT 5341 CCCTGTTGCT GGAGCAAAGC GCGGGGGTGT GGCTCTCCTG TCTCTGGCTT TGCTGTCAAC 5401 AGGCGAGATT GGCAAGTAGG GCGACCAACC GGCCTGGTTT GCTCGGGACC GGGGTTTCCT 5461 GGAAGGTGGG ACTTTCAATG CTAAAACTGG GACAGTCCTG GGCAAACAAA TCAGTACGGT 5521 TGGCCACCCT ATCTTTGTGT GGGCATCTGC TAACTGAGTC ATGGGCCTGT CTGGTTTATT 5581 TATTTATTTT TTTTCTGCCT GAGTAACAGA AGAATGTGTT AAACATATGG AGTATGGAAG 5641 AAGAAGGAGA GGGGAGATAG GATTTTTATG TTATAGATTG GAAGAAGACA TCCTTGATGA 5701 AGGAGATGTT TTTACTTTGG AGAAGGACTG CCTCAGCCGC GAAAGACCAA GGGGTGGCAC 5761 CACAGGGAGA AGTCTGCACT GCGCTTGTCA GGACGGCCAG TGGGGGGCTG AGATCGAGTT 5821 GTAGGACTAA AGGATAGACA TGCAGAAATG CATTTTAAAA ATCTGTTAGC TGGACCAGAC 5881 CGACAATGTA ACATAATTGC CAAAGCTTTG GTTCGTGACC TGAGGTTATG TTTGGTATGA 5941 AAAGGTCACA TTTTATATTC AGTTTTCTGA AGTTTTGGTT GCATAACCAA CCTGTGGAAG 6001 GCATGAACAC CCATGTGCGC CCTAACCAAA GGTTTTTCTG AATCATCCTT CACATGAGAA 6061 TTCCTAATGG GACCAAGTAC AGTACTGTGG TCCAACATAA ACACACAAGT CAGGCTGAGA 6121 GAATCTCAGA AGGTTGTGGA AGGGTCTATC TACTTTGGGA GCATTTTGCA GAGGAAGAAA 6181 CTGAGGTCCT GGCAGGTTGC ATTCTCCTGA TGGCAAAATG CAGCTCTTCC TATATGTATA 6241 CCCTGAATCT CCGCCCCCTT CCCCTCAGAT GCCCCCTGTC AGTTCCCCCA GCTGCTAAAT 6301 ATAGCTGTCT GTGGCTGGCT GCGTATGCAA CCGCACACCC CATTCTATCT GCCCTATCTC 6361 GGTTACAGTG TAGTCCTCCC CAGGGTCATC CTATGTACAC ACTACGTATT TCTAGCCAAC 6421 GAGGAGGGGG AATCAAACAG AAAGAGAGAC AAACAGAGAT ATATCGGAGT CTGGCACGGG 6481 GCACATAAGG CAGCACATTA GAGAAAGCCG GCCCCTGGAT CCGTCTTTCG CGTTTATTTT 6541 AAGCCCAGTC TTCCCTGGGC CACCTTTAGC AGATCCTCGT GCGCCCCCGC CCCCTGGCCG 6601 TGAAACTCAG CCTCTATCCA GCAGCGACGA CAAGTAAAGT AAAGTTCAGG GAAGCTGCTC 6661 TTTGGGATCG CTCCAAATCG AGTTGTGCCT GGAGTGATGT TTAAGCCAAT GTCAGGGCAA 6721 GGCAACAGTC CCTGGCCGTC CTCCAGCACC TTTGTAATGC ATATGAGCTC GGGAGACCAG 6781 TACTTAAAGT TGGAGGCCCG GGAGCCCAGG AGCTGGCGGA GGGCGTTCGT CCTGGGACTG 6841 CACTTGCTCC CGTCGGGTCG CCCGGCTTCA CCGGACCCGC AGGCTCCCGG GGCAGGGCCG 6901 GGGCCAGAGC TggatccCAC CATGGAAGAT GCCAAAAACA TTAAGAAGGG CCCAGCGCCA 6961 TTCTACCCAC TCGAAGACGG GACCGCCGGC GAGCAGCTGC ACAAAGCCAT GAAGCGCTAC 7021 GCCCTGGTGC CCGGCACCAT CGCCTTTACC GACGCACATA TCGAGGTGGA CATTACCTAC 7081 GCCGAGTACT TCGAGATGAG CGTTCGGCTG GCAGAAGCTA TGAAGCGCTA TGGGCTGAAT 7141 ACAAACCATC GGATCGTGGT GTGCAGCGAG AATAGCTTGC AGTTCTTCAT GCCCGTGTTG 7201 GGTGCCCTGT TCATCGGTGT GGCTGTGGCC CCAGCTAACG ACATCTACAA CGAGCGCGAG 7261 CTGCTGAACA GCATGGGCAT CAGCCAGCCC ACCGTCGTAT TCGTGAGCAA GAAAGGGCTG 7321 CAAAAGATCC TCAACGTGCA AAAGAAGCTA CCGATCATAC AAAAGATCAT CATCATGGAT 7381 AGCAAGACCG ACTACCAGGG CTTCCAAAGC ATGTACACCT TCGTGACTTC CCATTTGCCA 7441 CCCGGCTTCA ACGAGTACGA CTTCGTGCCC GAGAGCTTCG ACCGGGACAA AACCATCGCC 7501 CTGATCATGA ACAGTAGTGG CAGTACCGGA TTGCCCAAGG GCGTAGCCCT ACCGCACCGC 7561 ACCGCTTGTG TCCGATTCAG TCATGCCCGC GACCCCATCT TCGGCAACCA GATCATCCCC 7621 GACACCGCTA TCCTCAGCGT GGTGCCATTT CACCACGGCT TCGGCATGTT CACCACGCTG 7681 GGCTACTTGA TCTGCGGCTT TCGGGTCGTG CTCATGTACC GCTTCGAGGA GGAGCTATTC 7741 TTGCGCAGCT TGCAAGACTA TAAGATTCAA TCTGCCCTGC TGGTGCCCAC ACTATTTAGC 7801 TTCTTCGCTA AGAGCACTCT CATCGACAAG TACGACCTAA GCAACTTGCA CGAGATCGCC 7861 AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA GGTGAGGCCG TGGCCAAACG CTTCCACCTA 7921 CCAGGCATCC GCCAGGGCTA CGGCCTGACA GAAACAACCA GCGCCATTCT GATCACCCCC 7981 GAAGGGGACG ACAAGCCTGG CGCAGTAGGC AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG 8041 GTGGACTTGG ACACCGGTAA GACACTGGGT GTGAACCAGC GCGGCGAGCT GTGCGTCCGT 8101 GGCCCCATGA TCATGAGCGG CTACGTTAAC AACCCCGAGG CTACAAACGC TCTCATCGAC 8161 AAGGACGGCT GGCTGCACAG CGGCGACATC GCCTACTGGG ACGAGGACGA GCACTTCTTC 8221 ATCGTGGACC GGCTGAAGAG CCTGATCAAA TACAAGGGCT ACCAGGTAGC CCCAGCCGAA 8281 CTGGAGAGCA TCCTGCTGCA ACACCCCAAC ATCTTCGACG CCGGGGTCGC CGGCCTGCCC 8341 GACGACGATG CCGGCGAGCT GCCCGCCGCA GTCGTCGTGC TGGAACACGG TAAAACCATG 8401 ACCGAGAAGG AGATCGTGGA CTATGTGGCC AGCCAGGTTA CAACCGCCAA GAAGCTGCGC 8461 GGTGGTGTTG TGTTCGTGGA CGAGGTGCCT AAAGGACTGA CCGGCAAGTT GGACGCCCGC 8521 AAGATCCGCG AGATTCTCAT TAAGGCCAAG AAGGGCGGCA AGATCGCCGT GTAATTCTAG 8581 TTGTTTAAAC GAGCTCGCTA GCCTCGAGTC TAGAGTCGAC CTGCAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.