pLV2-PPM1A-3×FLAG-Puro plasmid

pLV2-PPM1A-3×FLAG-Puro plasmid

PVT93952

pLV2-PPM1A-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-PPM1A(human)-3×FLAG-Puro
Alias: NM_021003.5;PP2C-ALPHA; PP2CA; PP2Calpha
Gene lenght: 1146bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-PPM1A-3×FLAG-Puro plasmid Description

pLV2-PPM1A-3×FLAG-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-PPM1A-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgggagc atttttagac aagccaaaga tggaaaagca 2881 taatgcccag gggcagggta atgggttgcg atatgggcta agcagcatgc aaggctggcg 2941 tgttgaaatg gaggatgcac atacggctgt gatcggtttg ccaagtggac ttgaatcgtg 3001 gtcattcttt gctgtgtatg atgggcatgc tggttctcag gttgccaaat actgctgtga 3061 gcatttgtta gatcacatca ccaataacca ggattttaaa gggtctgcag gagcaccttc 3121 tgtggaaaat gtaaagaatg gaatcagaac aggttttctg gagattgatg aacacatgag 3181 agttatgtca gagaagaaac atggtgcaga tagaagtggg tcaacagctg taggtgtctt 3241 aatttctccc caacatactt atttcattaa ctgtggagac tcaagaggtt tactttgtag 3301 gaacaggaaa gttcatttct tcacacaaga tcacaaacca agtaatccgc tggagaaaga 3361 acgaattcag aatgcaggtg gctctgtaat gattcagcgt gtgaatggct ctctggctgt 3421 atcgagggcc cttggggatt ttgattacaa atgtgtccat ggaaaaggtc ctactgagca 3481 gcttgtctca ccagagcctg aagtccatga tattgaaaga tctgaagaag atgatcagtt 3541 cattatcctt gcatgtgatg gtatctggga tgttatggga aatgaagagc tctgtgattt 3601 tgtaagatcc agacttgaag tcactgatga ccttgagaaa gtttgcaatg aagtagtcga 3661 cacctgtttg tataagggaa gtcgagacaa catgagtgtg attttgatct gttttccaaa 3721 tgcacccaaa gtatcgccag aagcagtgaa gaaggaggca gagttggaca agtacctgga 3781 atgcagagta gaagaaatca taaagaagca gggggaaggc gtccccgact tagtccatgt 3841 gatgcgcaca ttagcgagtg agaacatccc cagcctccca ccagggggtg aattggcaag 3901 caagaggaat gttattgaag ccgtttacaa tagactgaat ccttacaaaa atgacgacac 3961 tgactctaca tcaacagatg atatgtgggg atccGACTAC AAAGACCATG ACGGTGATTA 4021 TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG tagtctagat aattctaccg 4081 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 4141 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 4201 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 4261 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 4321 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 4381 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 4441 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 4501 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 4561 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 4621 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 4681 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 4741 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 4801 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 4861 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 4921 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 4981 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 5041 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 5101 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 5161 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 5221 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5281 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5341 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5401 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5461 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5521 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 5581 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 5641 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 5701 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 5761 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 5821 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 5881 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 5941 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 6001 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 6061 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 6121 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 6181 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 6241 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 6301 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 6361 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 6421 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 6481 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6541 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6601 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6661 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 6721 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 6781 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 6841 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 6901 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 6961 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 7021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 7081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 7141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 7201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 7261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 7321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 7381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 7441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 7501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 7561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 7621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 7681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 7741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 7801 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 7861 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 7921 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7981 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 8041 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 8101 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 8161 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 8221 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 8281 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 8341 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 8401 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 8461 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 8521 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 8581 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 8641 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 8701 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 8761 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 8821 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 8881 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 8941 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 9001 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 9061 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 9121 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 9181 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 9241 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.