pTTLL1-Fluc plasmid

pTTLL1-Fluc plasmid

PVT96320

pTTLL1-Fluc plasmid Information

Plasmid name: pTTLL1(human)-Fluc
Alias: -
Gene lenght: 2100bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: TTLL1
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pTTLL1-Fluc plasmid Description

pTTLL1-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pTTLL1-Fluc plasmid Sequence

1 ggtaccCCAC TATTTTTTAA AAAGTGGTGA TAACACCATT AACTCAGCAG AGTTCTTGTG 61 GGGATTCATT GAGGGGAGCT GTGGCCAGCT TCTTAGGGTA AGGCCAGCCT CATGGGCCTG 121 TGACCTGTAC AGAACCATAG GACCCCGTGC TCAGACAAGC CCTGAAGTTA GTTTAATGCT 181 GCTGTCGCCA TCTTAAAGTT CTTTTTTTTT TTTTAAGACG GAGTCTCGCC CTGTTGCCCA 241 GGCTGGAGTG CAATAGTGCG ATCTCGGCTC ACTGTAACCT CTGTCTCCCA GGCTCAAACA 301 ATTCTCCTGC CTCAGCCTCC CAAGTAGCTG GGATTACAAG TGCGCACCAC CATGCCCGGC 361 AAATTTTTGT ATTTTTAGTA AAGACGGGGT TTCACCATGT TGGCCAGGCT GGTCTCAAAC 421 TGCTGACCTT AGGTGATCCA CCCGCCTCGG CCTCCCAAAG TGCTGGGATT ACAGGCGTAA 481 GGCACCATGC CCTGCCTTAA AATTCTTTTT TTGTTGTTGT TTTTTTTAAC TTTTTTTCTC 541 TAAGAGACAG AGTCTTGTTC TGTTGGCCAG GCTGTAGTGT AGTGGCGGGA TCATAGCTCA 601 CTGCAGCCTC AATCTCCTAG GCTCAAGTGA TCCACCTCAG CCTCCTGAGT AACTGAGTAG 661 GACTGCTGGC TACGCCCCCA TACCCGGCTG ATTAAAAATA TTTTTTCTTT TTTTTTTAGA 721 AATGGGGTCT TGCTATATTG GCCAGGCTGG TCTGGAACTC CTGACCTCAA GTGATCCTCC 781 CATCTTGGCC TCCCAAAGTA CAGGGATTGC AGGCATGAGG CAACATGCTG GAAGGCTACA 841 ATGTTTATTG AACACTTTAC TATGCCAGGC CCTAACCACG TCTTGCAAGC CTTCACTCAT 901 CTCCTGGGTT TAAGGTGAGA ACACTGTAGT CGGAAGCCTT GGGACTTCTC AAGGCCTCAC 961 AACCAGACAG CAGCTGAAGG GTATGAATTG GCAATTTGGA CTCCAGATAA GTGTTGGTTC 1021 CACTAGATGG TGCCAAAAGT AAATGATGAT GCGCTGAATG TGCTCTGGGC CTGGGAGGCG 1081 GTTGTGTGGG AGATTATAGG AAGTGATGTT AGCAAAGCTC CAATCATCAC TAAGAAAGCA 1141 CACCACCACC CCTCCTGAGA GGCAGCAGCA TGAATGGTCA GGTGTCCTGG GTTCAAAGCC 1201 AGACTCTGTC ACTTACAAGT TGCACAAGTT TGCATAACTC TTTAAATTGA TGAAATTAAA 1261 AATGAATAAT TGTATAGAGA ATTCGAGGTA ACACAGGTCT ATAACGAGAA TAATAAACGT 1321 CTTTTTTTTT TTTTTTTGAG GCGGAGTCTT GTTCTGTCGC CCAGGCTTGA GTGCAGTGGC 1381 ACGATCTTGG CTCACTGCAA CCTCTGTCTC CCGGGTTCAA GCGATTCTCT TGCCTCAGCC 1441 TAGCCAGGAT GGTCTCGATC TCCTGACCTC GCGACCCACC CGCCTCTGCC TCCCAAAGTG 1501 CTGGGATTAC AGGCGTGAGC CACCGAGTCT GGCCCAGATA AACACTTTTT AAAAAAAATT 1561 ATTTTATTTT TATGTTATTA TTATTATTAT TATTTGAGAT GGAGTCTCGC TCTATCGCCC 1621 AGGCTGGAGT GCAGTGGCGC AACATCACCA TTTCTTCAGA TAAGGAAGCC GAGGTAGAAG 1681 GGGTAGGAGA GGCTAAACAG CGGATTGAGA CCGAACCCAG GATTCGGACC TAATCATCAC 1741 ACCGCTCTAC CTTTCTTTTT AGACTTAATC CCGTGCTCCA GACCCTTCTC AATCTCCTTT 1801 CTGGGCCCCG AGAGGGTGGA CGGCCTGGCA TTTCCTAGGA TCCAAGCCCT GGGCATAGCG 1861 GCCGCCCGAG GTATGGAGCC CAAAGGAGCC CCAGGCAAGT CTGACTCCGG AAATAGTCGC 1921 AGCGCCGGCG GTCGCGTCCT GGGTTGCCAG GGCGCCGCCC TGGAAGTAGA GTGCAGCGCG 1981 GGGCGGGGCG GGCCGGTGCG CCTGGCAGTC GGCTGCGTAC TCCTGCGAGC GCCGGAGAGG 2041 GGCTGGAGGC GGGGCGAGGG CGCGGCTCGA GGCATCCCTG CAGGGCGCTC CTCCGAGTCC 2101 CTCGGCaagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca 2161 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc 2221 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata 2281 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta 2341 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc 2401 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg 2461 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt 2521 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc 2581 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt 2641 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg 2701 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag 2761 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt 2821 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt 2881 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata 2941 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc 3001 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat 3061 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg 3121 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat 3181 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc 3241 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa 3301 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag 3361 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg 3421 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct 3481 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg 3541 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt 3601 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa 3661 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta 3721 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa 3781 agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat 3841 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 3901 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 3961 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 4021 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg 4081 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca 4141 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc 4201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 4321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 4381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 4501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4561 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 5101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 5161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 5221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 5281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 5341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 5401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 5461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 5521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 5581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 5641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 5701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 5761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 5821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 5881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 5941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 6121 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 6181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 6241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 6301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 6361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 6421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 6481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 6541 atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc cattcaggct 6601 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct 6661 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata 6721 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac 6781 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca 6841 agtgcaggtg ccagaacatt tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.