pCMV-USP36-3×HA-Neo plasmid

pCMV-USP36-3×HA-Neo plasmid

PVT96297

pCMV-USP36-3×HA-Neo plasmid Information

Plasmid name: pCMV-USP36(human)-3×HA-Neo
Alias: NM_001385174.1;DUB1
Gene lenght: 3369bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-USP36-3×HA-Neo plasmid Description

pCMV-USP36-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-USP36-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atgccaatag tggataagtt gaaggaggcc 961 ctgaaacccg gccgcaagga ctcggctgat gatggagaac tggggaagct tcttgcctcc 1021 tctgccaaga aggtcctttt acagaaaatc gagttcgagc cagccagcaa gagcttctcc 1081 taccagctgg aggccttaaa gagcaaatat gtgttgctca accccaaaac agagggagct 1141 agtcgccaca agagtggaga tgacccaccg gccaggagac agggcagtga gcacacgtat 1201 gagagctgtg gtgacggagt cccagccccg cagaaagtgc ttttccccac ggagcgactg 1261 tctctgaggt gggagcgggt cttccgcgtg ggcgcaggac tccacaacct tggcaacacc 1321 tgctttctca atgccaccat ccagtgcttg acctacacac cacctctagc caactacctg 1381 ctctccaagg agcatgctcg cagctgccac cagggaagct tctgcatgct gtgtgtcatg 1441 cagaaccaca ttgtccaggc cttcgccaac agcggcaacg ccatcaagcc cgtctccttc 1501 atccgagacc tgaaaaagat cgcccgacac ttccgctttg ggaaccagga ggacgcgcat 1561 gagttcctgc ggtacaccat cgacgccatg cagaaagcct gcctgaatgg ctgtgccaag 1621 ttggatcgtc aaacgcaggc tactaccttg gtccatcaaa tttttggagg gtatctcaga 1681 tcacgcgtga agtgctccgt gtgcaagagc gtctcggaca cctacgaccc ctacttggac 1741 gtcgcgctgg agatccggca agctgcgaat attgtgcgtg ctctggaact ttttgtgaaa 1801 gcagatgtcc tgagtggaga gaatgcctac atgtgtgcta aatgcaagaa gaaggttcca 1861 gccagcaagc gcttcaccat ccacagaaca tccaacgtct taaccctttc cctcaagcgc 1921 tttgccaact tcagcggggg gaagatcacc aaggatgtag gctatccgga attcctcaac 1981 atacgtccgt atatgtccca gaataatggt gatcctgtca tgtatggact ctatgctgtc 2041 ctggtgcact cgggctacag ctgccatgcc gggcactatt actgctacgt gaaggcaagc 2101 aatggacagt ggtaccagat gaatgattcc ttggtccatt ccagcaacgt caaggtggtt 2161 ctgaaccagc aggcctacgt gctgttctat ctgcgaattc caggctctaa gaaaagtccc 2221 gagggcctca tctccaggac aggctcctcc tcccttcccg gccgcccgag tgtgattcca 2281 gatcactcca agaagaacat cggcaatggg attatttcct ccccactgac tggaaagcga 2341 caagactctg ggacgatgaa gaagccgcac accactgaag agattggtgt gcccatatcc 2401 aggaatggct ccaccctggg cctgaagtcc cagaacggct gcattcctcc aaagctgccc 2461 tcggggtccc cttcccccaa actctcccag acacccacac acatgccaac catcctagac 2521 gaccctggaa agaaggtgaa gaagccagct cctccacagc acttttcccc cagaactgct 2581 caggggctgc ctgggaccag caactcgaat agcagcagat ctgggagcca aaggcagggc 2641 tcctgggaca gcagggatgt tgtcctctct acctcaccta agctcctggc tacagccact 2701 gccaacgggc atgggctgaa ggggaacgac gagagcgctg gcctcgacag gaggggctcc 2761 agcagctcca gcccagagca ctcggccagc agcgactcca ccaaggcccc ccagaccccc 2821 aggagtggag cggcccatct ctgcgattct caggaaacga actgttccac cgctggccac 2881 tccaaaacgc cgccaagtgg agcagattct aagacggtga agctgaagtc ccctgtcctg 2941 agcaacacca ccactgagcc tgcaagcacc atgtctcctc caccagccaa aaaactggcc 3001 ctttctgcca agaaggccag caccctgtgg agggcgaccg gcaatgacct ccgtccacct 3061 cccccctcac catcctccga cctcacccac cccatgaaaa cctctcaccc cgtcgttgcc 3121 tccacttggc ccgtccatag agccagggct gtgtcacctg ctccccaatc atccagccgc 3181 ctgcaacccc ccttcagccc ccaccccaca ttgctgtcca gtacccccaa gcccccaggg 3241 acgtcagaac cacggagctg ctcctccatc tcgacggcgc tgcctcaggt caacgaggac 3301 cttgtgtctc ttccacacca gttgccagag gccagtgagc ccccccagag cccctctgag 3361 aagaggaaaa agacctttgt gggagagccg cagaggctgg gctcagagac gcgcctccca 3421 cagcacatca gggaggccac tgcggctccc cacgggaaga ggaagaggaa gaagaagaag 3481 cgcccggagg acacagctgc cagcgccctg caggaggggc agacacagag acagcctggg 3541 agccccatgt acaggaggga gggccaggca cagctgcccg ctgtcagacg gcaggaagat 3601 ggcacacagc cacaggtgaa tggccagcag gtgggatgtg ttacggacgg ccaccacgcg 3661 agcagcagga agcggaggag gaaaggagca gaaggtcttg gtgaagaagg cggcctgcac 3721 caggacccac ttcggcacag ctgctctccc atgggtgatg gtgatccaga ggccatggaa 3781 gagtctccaa ggaaaaagaa aaagaaaaaa agaaagcagg agacacagcg ggcagtagaa 3841 gaggatgggc atctcaaatg cccaaggagt gccaagcccc aagatgctgt tgtccccgag 3901 tccagcagct gcgcaccatc cgcgaatggc tggtgtcctg gggaccgcat ggggctgagc 3961 caggcccctc ctgtgtcttg gaatggagag cgggagtctg atgtggtcca ggaactgctc 4021 aaatactcat ctgataaagc ttacgggaga aaagttctga cctgggatgg caagatgtcg 4081 gcggtcagtc aggatgctat tgaagacagc agacaggccc ggactgagac cgtggttgat 4141 gactgggacg aagagtttga ccgagggaag gaaaagaaaa ttaaaaaatt taagagagag 4201 aagaggagaa acttcaacgc cttccagaaa cttcagactc gacggaactt ctggtctgtg 4261 actcacccag caaaggctgc cagcctcagc tatcgccgcC TCGAGTACCC ATACGACGTA 4321 CCTGATTATG CAACTTATCC TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC 4381 CCAGACTACG CAtgaGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT 4441 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 4501 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT 4561 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC 4621 AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC 4681 TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 4741 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 4801 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 4861 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 4921 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 4981 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 5041 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 5101 ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA 5161 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 5221 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 5281 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 5341 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 5401 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 5461 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT 5521 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG 5581 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG 5641 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 5701 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC 5761 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG 5821 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA 5881 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC 5941 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 6001 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG 6061 CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA 6121 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG 6181 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT 6241 GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT 6301 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA 6361 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 6421 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 6481 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 6541 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 6601 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 6661 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 6721 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 6781 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 6841 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 6901 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 6961 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 7021 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 7081 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 7141 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 7201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 7261 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 7321 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 7381 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 7441 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 7501 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 7561 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT 7621 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 7681 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 7741 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 7801 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 7861 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 7921 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 7981 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 8041 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 8101 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 8161 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 8221 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 8281 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 8341 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 8401 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 8461 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 8521 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 8581 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 8641 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 8701 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 8761 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 8821 TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.