pCMV-ERBB2-tdTomato-Neo plasmi

pCMV-ERBB2-tdTomato-Neo plasmid

PVT92720

pCMV-ERBB2-tdTomato-Neo plasmid Information

Plasmid name: pCMV-ERBB2(human)-tdTomato-Neo
Alias: NM_004448.4;c-ERB-2; c-ERB2; CD340; HER-2; HER-2/neu;HER2; MLN 19; MLN-19; NEU; NGL; p185(erbB2); TKR1; VSCN2;
Gene lenght: 3765bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ERBB2-tdTomato-Neo plasmid Description

pCMV-ERBB2-tdTomato-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-ERBB2-tdTomato-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc 601 atggagctgg cggccttgtg ccgctggggg ctcctcctcg ccctcttgcc ccccggagcc 661 gcgagcaccc aagtgtgcac cggcacagac atgaagctgc ggctccctgc cagtcccgag 721 acccacctgg acatgctccg ccacctctac cagggctgcc aggtggtgca gggaaacctg 781 gaactcacct acctgcccac caatgccagc ctgtccttcc tgcaggatat ccaggaggtg 841 cagggctacg tgctcatcgc tcacaaccaa gtgaggcagg tcccactgca gaggctgcgg 901 attgtgcgag gcacccagct ctttgaggac aactatgccc tggccgtgct agacaatgga 961 gacccgctga acaataccac ccctgtcaca ggggcctccc caggaggcct gcgggagctg 1021 cagcttcgaa gcctcacaga gatcttgaaa ggaggggtct tgatccagcg gaacccccag 1081 ctctgctacc aggacacgat tttgtggaag gacatcttcc acaagaacaa ccagctggct 1141 ctcacactga tagacaccaa ccgctctcgg gcctgccacc cctgttctcc gatgtgtaag 1201 ggctcccgct gctggggaga gagttctgag gattgtcaga gcctgacgcg cactgtctgt 1261 gccggtggct gtgcccgctg caaggggcca ctgcccactg actgctgcca tgagcagtgt 1321 gctgccggct gcacgggccc caagcactct gactgcctgg cctgcctcca cttcaaccac 1381 agtggcatct gtgagctgca ctgcccagcc ctggtcacct acaacacaga cacgtttgag 1441 tccatgccca atcccgaggg ccggtataca ttcggcgcca gctgtgtgac tgcctgtccc 1501 tacaactacc tttctacgga cgtgggatcc tgcaccctcg tctgccccct gcacaaccaa 1561 gaggtgacag cagaggatgg aacacagcgg tgtgagaagt gcagcaagcc ctgtgcccga 1621 gtgtgctatg gtctgggcat ggagcacttg cgagaggtga gggcagttac cagtgccaat 1681 atccaggagt ttgctggctg caagaagatc tttgggagcc tggcatttct gccggagagc 1741 tttgatgggg acccagcctc caacactgcc ccgctccagc cagagcagct ccaagtgttt 1801 gagactctgg aagagatcac aggttaccta tacatctcag catggccgga cagcctgcct 1861 gacctcagcg tcttccagaa cctgcaagta atccggggac gaattctgca caatggcgcc 1921 tactcgctga ccctgcaagg gctgggcatc agctggctgg ggctgcgctc actgagggaa 1981 ctgggcagtg gactggccct catccaccat aacacccacc tctgcttcgt gcacacggtg 2041 ccctgggacc agctctttcg gaacccgcac caagctctgc tccacactgc caaccggcca 2101 gaggacgagt gtgtgggcga gggcctggcc tgccaccagc tgtgcgcccg agggcactgc 2161 tggggtccag ggcccaccca gtgtgtcaac tgcagccagt tccttcgggg ccaggagtgc 2221 gtggaggaat gccgagtact gcaggggctc cccagggagt atgtgaatgc caggcactgt 2281 ttgccgtgcc accctgagtg tcagccccag aatggctcag tgacctgttt tggaccggag 2341 gctgaccagt gtgtggcctg tgcccactat aaggaccctc ccttctgcgt ggcccgctgc 2401 cccagcggtg tgaaacctga cctctcctac atgcccatct ggaagtttcc agatgaggag 2461 ggcgcatgcc agccttgccc catcaactgc acccactcct gtgtggacct ggatgacaag 2521 ggctgccccg ccgagcagag agccagccct ctgacgtcca tcatctctgc ggtggttggc 2581 attctgctgg tcgtggtctt gggggtggtc tttgggatcc tcatcaagcg acggcagcag 2641 aagatccgga agtacacgat gcggagactg ctgcaggaaa cggagctggt ggagccgctg 2701 acacctagcg gagcgatgcc caaccaggcg cagatgcgga tcctgaaaga gacggagctg 2761 aggaaggtga aggtgcttgg atctggcgct tttggcacag tctacaaggg catctggatc 2821 cctgatgggg agaatgtgaa aattccagtg gccatcaaag tgttgaggga aaacacatcc 2881 cccaaagcca acaaagaaat cttagacgaa gcatacgtga tggctggtgt gggctcccca 2941 tatgtctccc gccttctggg catctgcctg acatccacgg tgcagctggt gacacagctt 3001 atgccctatg gctgcctctt agaccatgtc cgggaaaacc gcggacgcct gggctcccag 3061 gacctgctga actggtgtat gcagattgcc aaggggatga gctacctgga ggatgtgcgg 3121 ctcgtacaca gggacttggc cgctcggaac gtgctggtca agagtcccaa ccatgtcaaa 3181 attacagact tcgggctggc tcggctgctg gacattgacg agacagagta ccatgcagat 3241 gggggcaagg tgcccatcaa gtggatggcg ctggagtcca ttctccgccg gcggttcacc 3301 caccagagtg atgtgtggag ttatggtgtg actgtgtggg agctgatgac ttttggggcc 3361 aaaccttacg atgggatccc agcccgggag atccctgacc tgctggaaaa gggggagcgg 3421 ctgccccagc cccccatctg caccattgat gtctacatga tcatggtcaa atgttggatg 3481 attgactctg aatgtcggcc aagattccgg gagttggtgt ctgaattctc ccgcatggcc 3541 agggaccccc agcgctttgt ggtcatccag aatgaggact tgggcccagc cagtcccttg 3601 gacagcacct tctaccgctc actgctggag gacgatgaca tgggggacct ggtggatgct 3661 gaggagtatc tggtacccca gcagggcttc ttctgtccag accctgcccc gggcgctggg 3721 ggcatggtcc accacaggca ccgcagctca tctaccagga gtggcggtgg ggacctgaca 3781 ctagggctgg agccctctga agaggaggcc cccaggtctc cactggcacc ctccgaaggg 3841 gctggctccg atgtatttga tggtgacctg ggaatggggg cagccaaggg gctgcaaagc 3901 ctccccacac atgaccccag ccctctacag cggtacagtg aggaccccac agtacccctg 3961 ccctctgaga ctgatggcta cgttgccccc ctgacctgca gcccccagcc tgaatatgtg 4021 aaccagccag atgttcggcc ccagccccct tcgccccgag agggccctct gcctgctgcc 4081 cgacctgctg gtgccactct ggaaaggccc aagactctct ccccagggaa gaatggggtc 4141 gtcaaagacg tttttgcctt tgggggtgcc gtggagaacc ccgagtactt gacaccccag 4201 ggaggagctg cccctcagcc ccaccctcct cctgccttca gcccagcctt cgacaacctc 4261 tattactggg accaggaccc accagagcgg ggggctccac ccagcacctt caaagggaca 4321 cctacggcag agaacccaga gtacctgggt ctggacgtgc cagtgGGAGG AGGGGGAAGC 4381 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc 4441 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc 4501 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc 4561 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg 4621 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg 4681 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg 4741 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc 4801 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc 4861 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac 4921 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac 4981 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag 5041 tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc 5101 agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc 5161 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc 5221 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc 5281 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg 5341 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc 5401 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc 5461 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc 5521 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac 5581 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc 5641 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc 5701 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg 5761 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg 5821 tacaagtagg cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt 5881 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 5941 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6001 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6061 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 6121 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 6181 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 6241 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 6301 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 6361 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 6421 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 6481 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 6541 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 6601 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 6661 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 6721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 6781 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 6841 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 6901 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 6961 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 7021 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 7081 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 7141 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 7201 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 7261 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 7321 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 7381 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 7441 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 7501 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 7561 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 7621 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 7681 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 7741 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 7801 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 7861 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 7921 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 7981 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 8041 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 8101 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 8161 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 8221 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 8281 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 8341 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 8401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 8461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 8521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 8581 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 8641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 8701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 8761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 8821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 8881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 8941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 9001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 9061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 9121 gttatcccct gattctgtgg ataaccgtat taccgccatg cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.