pLV3-CMV-EGFP-RIPK4-3×FLAG-Pur

pLV3-CMV-EGFP-RIPK4-3×FLAG-Puro plasmid

PVT96285

pLV3-CMV-EGFP-RIPK4-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-EGFP-RIPK4(human)-3×FLAG-Puro
Alias: NM_020639.3;ANKK2; ANKRD3; CHANDS; DIK; NKRD3; PKK;PPS2; RIP4
Gene lenght: 2352bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EGFP-RIPK4-3×FLAG-Puro plasmid Description

pLV3-CMV-EGFP-RIPK4-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EGFP-RIPK4-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGATTCTA 2281 GAGCTAGCGA ATTCagccac catggtgagc aagggcgagg agctgttcac cggggtggtg 2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 3001 atggacgagc tgtacaagGG AGGAGGGGGA AGCggtggag gcggttctCT CGAGatggag 3061 ggcgacggcg ggaccccatg ggccctggcg ctgctgcgca ccttcgacgc gggcgagttc 3121 acgggctggg agaaggtggg ctcgggcggc ttcgggcagg tgtacaaggt gcgccatgtc 3181 cactggaaga cctggctggc catcaagtgc tcgcccagcc tgcacgtcga cgacagggag 3241 cgcatggagc ttttggaaga agccaagaag atggagatgg ccaagtttcg ctacatcctg 3301 cctgtgtatg gcatctgccg cgaacctgtc ggcctggtca tggagtacat ggagacgggc 3361 tccctggaaa agctgctggc ttcggagcca ttgccatggg atctccggtt ccgaatcatc 3421 cacgagacgg cggtgggcat gaacttcctg cactgcatgg ccccgccact cctgcacctg 3481 gacctcaagc ccgcgaacat cctgctggat gcccactacc acgtcaagat ttctgatttt 3541 ggtctggcca agtgcaacgg gctgtcccac tcgcatgacc tcagcatgga tggcctgttt 3601 ggcacaatcg cctacctccc tccagagcgc atcagggaga agagccggct cttcgacacc 3661 aagcacgatg tatacagctt tgcgatcgtc atctggggcg tgctcacaca gaagaagccg 3721 tttgcagatg agaagaacat cctgcacatc atggtgaagg tggtgaaggg ccaccgcccc 3781 gagctgccgc ccgtgtgcag agcccggccg cgcgcctgca gccacctgat acgcctcatg 3841 cagcggtgct ggcaggggga tccgcgagtt aggcccacct tccaagaaat tacttctgaa 3901 accgaggacc tgtgtgaaaa gcctgatgac gaagtgaaag aaactgctca tgatctggac 3961 gtgaaaagcc ccccggagcc caggagcgag gtggtgcctg cgaggctcaa gcgggcctct 4021 gcccccacct tcgataacga ctacagcctc tccgagctgc tctcacagct ggactctgga 4081 gtttcccagg ctgtcgaggg ccccgaggag ctcagccgca gctcctctga gtccaagctg 4141 ccatcgtccg gcagtgggaa gaggctctcg ggggtgtcct cggtggactc cgccttctct 4201 tccagaggat cactgtcgct gtcctttgag cgggaacctt caaccagcga tctgggcacc 4261 acagacgtcc agaagaagaa gcttgtggat gccatcgtgt ccggggacac cagcaaactg 4321 atgaagatcc tgcagccgca ggacgtggac ctggcactgg acagcggtgc cagcctgctg 4381 cacctggcgg tggaggccgg gcaagaggag tgcgccaagt ggctgctgct caacaatgcc 4441 aaccccaacc tgagcaaccg taggggctcc accccgttgc acatggccgt ggagaggagg 4501 gtgcggggtg tcgtggagct cctgctggcg cggaagatca gtgtcaacgc caaggatgag 4561 gaccagtgga cagccctcca ctttgcagcc cagaacgggg acgagtctag cacacggctg 4621 ctgttggaga agaacgcctc ggtcaacgag gtggactttg agggccggac gcccatgcac 4681 gtggcctgcc agcacgggca ggagaatatc gtgcgcatcc tgctgcgccg aggcgtggac 4741 gtgagcctgc agggcaagga tgcctggctg ccactgcact acgctgcctg gcagggccac 4801 ctgcccatcg tcaagctgct ggccaagcag ccgggggtga gtgtgaacgc ccagacgctg 4861 gatgggagga cgccattgca cctggccgca cagcgcgggc actaccgcgt ggcccgcatc 4921 ctcatcgacc tgtgctccga cgtcaacgtc tgcagcctgc tggcacagac acccctgcac 4981 gtggccgcgg agacggggca cacgagcact gccaggctgc tcctgcatcg gggcgctggc 5041 aaggaggcca tgacctcaga cggctacacc gctctgcacc tggctgcccg caacggacac 5101 ctggccactg tcaagctgct tgtcgaggag aaggccgatg tgctggcccg gggacccctg 5161 aaccagacgg cgctgcacct ggctgccgcc cacgggcact cggaggtggt ggaggagttg 5221 gtcagcgccg atgtcattga cctgttcgac gagcaggggc tcagcgcgct gcacctggcc 5281 gcccagggcc ggcacgcaca gacggtggag actctgctca ggcatggggc ccacatcaac 5341 ctgcagagcc tcaagttcca gggcggccat ggccccgccg ccacgctcct gcggcgaagc 5401 aagaccGGAT CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 5461 AAGGATGACG ATGACAAGTA GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG 5521 TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA 5581 ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC 5641 CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT 5701 CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT 5761 CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG 5821 CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC 5881 AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC 5941 TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG 6001 CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ATGACCGAGT ACAAGCCCAC 6061 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT 6121 CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG AGCGGGTCAC 6181 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC 6241 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT 6301 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA 6361 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC 6421 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG 6481 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA 6541 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG 6601 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTCGACAATC AACCTCTGGA 6661 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 6721 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT 6781 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG 6841 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC 6901 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA 6961 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA 7021 TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC 7081 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT 7141 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA 7201 GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGT ACCTTTAAGA CCAATGACTT 7261 ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA 7321 TTCACTCCCA ACGAAAATAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC 7381 AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA 7441 GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA 7501 GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC 7561 TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT 7621 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 7681 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 7741 TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG 7801 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA 7861 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC AGAGACGGCC CAAATTCGTA 7921 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 7981 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 8041 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA 8101 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC 8161 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 8221 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 8281 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 8341 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 8401 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 8461 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 8521 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 8581 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 8641 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 8701 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 8761 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 8821 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 8881 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 8941 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 9001 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 9061 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 9121 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 9181 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 9241 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 9301 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 9361 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC 9421 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC 9481 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 9541 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC 9601 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG 9661 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC 9721 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT 9781 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 9841 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA 9901 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT 9961 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG 10021 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA 10081 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC 10141 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 10201 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 10261 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 10321 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 10381 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC 10441 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC 10501 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGCCAAGC TG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.