pLV2-CMV-HA-Acadm-EGFP-Puro pl

pLV2-CMV-HA-Acadm-EGFP-Puro plasmid

PVT94117

pLV2-CMV-HA-Acadm-EGFP-Puro plasmid Information

Plasmid name: pLV2-CMV-HA-Acadm(mouse)-EGFP-Puro
Alias: NM_007382.5;MCAD;
Gene lenght: 1263bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-HA-Acadm-EGFP-Puro plasmid Description

pLV2-CMV-HA-Acadm-EGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-HA-Acadm-EGFP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gccaccatgT ACCCATACGA TGTTCCAGAT TACGCTgaat tcatggcagc 2881 ggcgttccgc agaggctgca gggtcctgag aagtgtttct cattttgagt gtcgaacaca 2941 acactcgaaa gcggctcaca agcaggagcc cggattaggg tttagttttg agttgacgga 3001 acagcagaaa gagtttcaag caactgcccg caagtttgcc agagaggaga ttatccccgt 3061 cgccccggaa tatgacaaaa gcggggagta cccgttccct ctcatcaaaa gagcctggga 3121 actcggcttg atcaacgcgc acattccgga aagttgcggt ggccttggcc tgggaacgtt 3181 cgatgcttgt ttaattaccg aagagttggc gtatgggtgt acaggggtgc aaactgctat 3241 tgaagcaaat tctttggggc aaatgcctgt gattcttgct ggaaatgatc aacaaaaaaa 3301 gaagtatttg gggaggatga cggagcagcc aatgatgtgt gcttactgtg tgacagagcc 3361 ctccgcaggc tctgatgtgg cggccattaa gaccaaagca gagaagaagg gtgacgagta 3421 tgttatcaac ggccagaaga tgtggataac caacggggga aaggccaact ggtatttctt 3481 gttggcacgt tctaacccag atcctaaagt acccgctagt aaagccttta ctggattcat 3541 tgtggaagcc gacaccccgg gaatacatat tggaaaaaag gaactaaaca tgggccagcg 3601 atgctctgac accagaggaa ttgctttcga agacgtcaga gtgcctaagg aaaatgtgtt 3661 aatcggtgaa ggagcaggtt tcaagatcgc aatgggtgct tttgatagaa ccagacctac 3721 agtcgcagct ggcgctgtcg ggctagccca gagagctcta gacgaagcca cgaagtatgc 3781 cctggatagg aagacatttg gaaagctgct agtggagcac caaggagttt catttctgct 3841 cgcagaaatg gcgatgaagg ttgaactcgc taggctcagt taccagagag cagcctggga 3901 ggttgactcc ggtcgccgga acacttacta tgcctcgatt gcaaaggcct ttgctggaga 3961 cattgccaat cagctagcca ctgacgccgt gcagattttc ggaggctatg gattcaacac 4021 agagtaccct gtggagaagc tgatgaggga cgccaagatc tatcagattt atgaaggtac 4081 tgcacaaatt cagaggctga tcatagctcg tgagcacatt gaaaagtata aaaatggtgg 4141 aggcggatca ggaggcggtg gatccgccac catggtgagc aagggcgagg agctgttcac 4201 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 4261 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 4321 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 4381 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 4441 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 4501 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 4561 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 4621 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 4681 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 4741 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 4801 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 4861 cactctcggc atggacgagc tgtacaagta gtctagataa ttctaccggg taggggaggc 4921 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 4981 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 5041 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 5101 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 5161 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 5221 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 5281 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 5341 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 5401 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc 5461 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5521 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5581 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5641 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5701 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5761 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5821 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5881 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5941 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 6001 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa 6061 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 6121 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 6181 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6241 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6301 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 6361 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 6421 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 6481 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 6541 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 6601 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa 6661 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta 6721 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca 6781 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa 6841 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa 6901 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc 6961 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa 7021 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga 7081 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg 7141 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg 7201 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc 7261 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg 7321 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 7381 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7441 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7501 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt 7561 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 7621 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 7681 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 7741 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 7801 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 7861 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7921 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 8041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 8101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 8161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 8221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 8281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 8461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 8821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 9061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9721 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 9781 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 9841 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 9901 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 9961 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 10021 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 10081 gaaattgtat ttgttaaata tgtactacaa acttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.