pTac-GST-JAK1 plasmid

pTac-GST-JAK1 plasmid

PVT96247

pTac-GST-JAK1 plasmid Information

Plasmid name: pTac-GST-JAK1(human)
Alias: NM_002227.4;AIIDE; JAK1A; JAK1B; JTK3
Gene lenght: 3465bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: High
Induction:

pTac-GST-JAK1 plasmid Description

pTac-GST-JAK1 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-JAK1 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc atgcagtatc 961 taaatataaa agaggactgc aatgccatgg ctttctgtgc taaaatgagg agctccaaga 1021 agactgaggt gaacctggag gcccctgagc caggggtgga agtgatcttc tatctgtcgg 1081 acagggagcc cctccggctg ggcagtggag agtacacagc agaggaactg tgcatcaggg 1141 ctgcacaggc atgccgtatc tctcctcttt gtcacaacct ctttgccctg tatgacgaga 1201 acaccaagct ctggtatgct ccaaatcgca ccatcaccgt tgatgacaag atgtccctcc 1261 ggctccacta ccggatgagg ttctatttca ccaattggca tggaaccaac gacaatgagc 1321 agtcagtgtg gcgtcattct ccaaagaagc agaaaaatgg ctacgagaaa aaaaagattc 1381 cagatgcaac ccctctcctt gatgccagct cactggagta tctgtttgct cagggacagt 1441 atgatttggt gaaatgcctg gctcctattc gagaccccaa gaccgagcag gatggacatg 1501 atattgagaa cgagtgtcta gggatggctg tcctggccat ctcacactat gccatgatga 1561 agaagatgca gttgccagaa ctgcccaagg acatcagcta caagcgatat attccagaaa 1621 cattgaataa gtccatcaga cagaggaacc ttctcaccag gatgcggata aataatgttt 1681 tcaaggattt cctaaaggaa tttaacaaca agaccatttg tgacagcagc gtgtccacgc 1741 atgacctgaa ggtgaaatac ttggctacct tggaaacttt gacaaaacat tacggtgctg 1801 aaatatttga gacttccatg ttactgattt catcagaaaa tgagatgaat tggtttcatt 1861 cgaatgacgg tggaaacgtt ctctactacg aagtgatggt gactgggaat cttggaatcc 1921 agtggaggca taaaccaaat gttgtttctg ttgaaaagga aaaaaataaa ctgaagcgga 1981 aaaaactgga aaataaacac aagaaggatg aggagaaaaa caagatccgg gaagagtgga 2041 acaatttttc ttacttccct gaaatcactc acattgtaat aaaggagtct gtggtcagca 2101 ttaacaagca ggacaacaag aaaatggaac tgaagctctc ttcccacgag gaggccttgt 2161 cctttgtgtc cctggtagat ggctacttcc ggctcacagc agatgcccat cattacctct 2221 gcaccgacgt ggcccccccg ttgatcgtcc acaacataca gaatggctgt catggtccaa 2281 tctgtacaga atacgccatc aataaattgc ggcaagaagg aagcgaggag gggatgtacg 2341 tgctgaggtg gagctgcacc gactttgaca acatcctcat gaccgtcacc tgctttgaga 2401 agtctgagca ggtgcagggt gcccagaagc agttcaagaa ctttcagatc gaggtgcaga 2461 agggccgcta cagtctgcac ggttcggacc gcagcttccc cagcttggga gacctcatga 2521 gccacctcaa gaagcagatc ctgcgcacgg ataacatcag cttcatgcta aaacgctgct 2581 gccagcccaa gccccgagaa atctccaacc tgctggtggc tactaagaaa gcccaggagt 2641 ggcagcccgt ctaccccatg agccagctga gtttcgatcg gatcctcaag aaggatctgg 2701 tgcagggcga gcaccttggg agaggcacga gaacacacat ctattctggg accctgatgg 2761 attacaagga tgacgaagga acttctgaag agaagaagat aaaagtgatc ctcaaagtct 2821 tagaccccag ccacagggat atttccctgg ccttcttcga ggcagccagc atgatgagac 2881 aggtctccca caaacacatc gtgtacctct atggcgtctg tgtccgcgac gtggagaata 2941 tcatggtgga agagtttgtg gaagggggtc ctctggatct cttcatgcac cggaaaagcg 3001 atgtccttac cacaccatgg aaattcaaag ttgccaaaca gctggccagt gccctgagct 3061 acttggagga taaagacctg gtccatggaa atgtgtgtac taaaaacctc ctcctggccc 3121 gtgagggcat cgacagtgag tgtggcccat tcatcaagct cagtgacccc ggcatcccca 3181 ttacggtgct gtctaggcaa gaatgcattg aacgaatccc atggattgct cctgagtgtg 3241 ttgaggactc caagaacctg agtgtggctg ctgacaagtg gagctttgga accacgctct 3301 gggaaatctg ctacaatggc gagatcccct tgaaagacaa gacgctgatt gagaaagaga 3361 gattctatga aagccggtgc aggccagtga caccatcatg taaggagctg gctgacctca 3421 tgacccgctg catgaactat gaccccaatc agaggccttt cttccgagcc atcatgagag 3481 acattaataa gcttgaagag cagaatccag atattgtttc agaaaaaaaa ccagcaactg 3541 aagtggaccc cacacatttt gaaaagcgct tcctaaagag gatccgtgac ttgggagagg 3601 gccactttgg gaaggttgag ctctgcaggt atgaccccga aggggacaat acaggggagc 3661 aggtggctgt taaatctctg aagcctgaga gtggaggtaa ccacatagct gatctgaaaa 3721 aggaaatcga gatcttaagg aacctctatc atgagaacat tgtgaagtac aaaggaatct 3781 gcacagaaga cggaggaaat ggtattaagc tcatcatgga atttctgcct tcgggaagcc 3841 ttaaggaata tcttccaaag aataagaaca aaataaacct caaacagcag ctaaaatatg 3901 ccgttcagat ttgtaagggg atggactatt tgggttctcg gcaatacgtt caccgggact 3961 tggcagcaag aaatgtcctt gttgagagtg aacaccaagt gaaaattgga gacttcggtt 4021 taaccaaagc aattgaaacc gataaggagt attacaccgt caaggatgac cgggacagcc 4081 ctgtgttttg gtatgctcca gaatgtttaa tgcaatctaa attttatatt gcctctgacg 4141 tctggtcttt tggagtcact ctgcatgagc tgctgactta ctgtgattca gattctagtc 4201 ccatggcttt gttcctgaaa atgataggcc caacccatgg ccagatgaca gtcacaagac 4261 ttgtgaatac gttaaaagaa ggaaaacgcc tgccgtgccc acctaactgt ccagatgagg 4321 tttatcaact tatgaggaaa tgctgggaat tccaaccatc caatcggaca agctttcaga 4381 accttattga aggatttgaa gcacttttaa aataactcga gcggccgcat cgtgactgac 4441 tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 4501 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 4561 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 4621 gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 4681 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 4741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 4801 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 4861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 4921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 4981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 5041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc 5101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 5161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 5221 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 5281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 5341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt 5401 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 5461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 5521 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 5581 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 5641 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 5701 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 5761 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 5821 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 5881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 5941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 6001 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 6061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 6121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 6181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 6241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 6301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 6361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 6421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 6481 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 6541 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 6601 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc 6661 tccttacgca tctgtgcggt atttcacacc gcataaattc cgacaccatc gaatggtgca 6721 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg 6781 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt 6841 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg 6901 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt 6961 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg 7021 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac 7081 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg 7141 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca 7201 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt 7261 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc 7321 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct 7381 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga 7441 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg 7501 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg 7561 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat 7621 gttatatccc gccgtcaacc accatcaaac aggattttcg cctgctgggg caaaccagcg 7681 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg 7741 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg 7801 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 7861 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 7921 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 7981 agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg actgggaaaa 8041 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 8101 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 8161 gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct 8221 tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc 8281 catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa 8341 tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca 8401 gacgcgaatt atttttgatg gcgttggaat t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.