pCMV-3×HA-ADORA2A-Neo plasmid

pCMV-3×HA-ADORA2A-Neo plasmid

PVT94242

pCMV-3×HA-ADORA2A-Neo plasmid Information

Plasmid name: pCMV-3×HA-ADORA2A(human)-Neo
Alias: NM_001278497.2;A2aR; ADORA2; RDC8
Gene lenght: 1239bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-ADORA2A-Neo plasmid Description

pCMV-3×HA-ADORA2A-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×HA-ADORA2A-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGTAC CCATACGACG TACCTGATTA TGCAACTTAT 961 CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCATCAGCG 1021 GAAAAGCTTa tgcccatcat gggctcctcg gtgtacatca cggtggagct ggccattgct 1081 gtgctggcca tcctgggcaa tgtgctggtg tgctgggccg tgtggctcaa cagcaacctg 1141 cagaacgtca ccaactactt tgtggtgtca ctggcggcgg ccgacatcgc agtgggtgtg 1201 ctcgccatcc cctttgccat caccatcagc accgggttct gcgctgcctg ccacggctgc 1261 ctcttcattg cctgcttcgt cctggtcctc acgcagagct ccatcttcag tctcctggcc 1321 atcgccattg accgctacat tgccatccgc atcccgctcc ggtacaatgg cttggtgacc 1381 ggcacgaggg ctaagggcat cattgccatc tgctgggtgc tgtcgtttgc catcggcctg 1441 actcccatgc taggttggaa caactgcggt cagccaaagg agggcaagaa ccactcccag 1501 ggctgcgggg agggccaagt ggcctgtctc tttgaggatg tggtccccat gaactacatg 1561 gtgtacttca acttctttgc ctgtgtgctg gtgcccctgc tgctcatgct gggtgtctat 1621 ttgcggatct tcctggcggc gcgacgacag ctgaagcaga tggagagcca gcctctgccg 1681 ggggagcggg cacggtccac actgcagaag gaggtccatg ctgccaagtc actggccatc 1741 attgtggggc tctttgccct ctgctggctg cccctacaca tcatcaactg cttcactttc 1801 ttctgccccg actgcagcca cgcccctctc tggctcatgt acctggccat cgtcctctcc 1861 cacaccaatt cggttgtgaa tcccttcatc tacgcctacc gtatccgcga gttccgccag 1921 accttccgca agatcattcg cagccacgtc ctgaggcagc aagaaccttt caaggcagct 1981 ggcaccagtg cccgggtctt ggcagctcat ggcagtgacg gagagcaggt cagcctccgt 2041 ctcaacggcc acccgccagg agtgtgggcc aacggcagtg ctccccaccc tgagcggagg 2101 cccaatggct atgccctggg gctggtgagt ggagggagtg cccaagagtc ccaggggaac 2161 acgggcctcc cagacgtgga gctccttagc catgagctca agggagtgtg cccagagccc 2221 cctggcctag atgaccccct ggcccaggat ggagcaggag tgtcctgaga attctgcaga 2281 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc 2341 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 2401 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 2461 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 2521 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 2581 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 2641 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 2701 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 2761 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 2821 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 2881 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 2941 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 3001 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 3061 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 3121 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 3181 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 3241 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 3301 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 3361 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 3421 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 3481 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 3541 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 3601 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 3661 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 3721 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 3781 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 3841 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 3901 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 3961 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 4021 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 4081 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 4141 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 4201 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 4261 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 4321 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 4381 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 4441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 4501 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 4561 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 4621 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 4681 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 4741 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 4801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 4861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 4921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 4981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 5041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 5101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 5161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 5221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 5281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 5341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 5401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 5461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 5521 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5581 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5641 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5701 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5761 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 5821 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5881 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5941 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6001 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 6061 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 6121 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 6181 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 6241 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 6301 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 6361 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 6421 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6481 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6541 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6601 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6661 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6721 tccccgaaaa gtgccacctg acgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.