pT3-EF1a-Slc41a3 plasmid

pT3-EF1a-Slc41a3 plasmid

PVT96237

pT3-EF1a-Slc41a3 plasmid Information

Plasmid name: pT3-EF1a-Slc41a3(mouse)
Alias: NM_027868.2;1010001P06Rik; SLC41A1-L2
Gene lenght: 1467bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pT3-EF1a-Slc41a3 plasmid Description

pT3-EF1a-Slc41a3 is a plasmid suitable for protein expression in mammalian cells.

pT3-EF1a-Slc41a3 plasmid Sequence

1 GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG 61 GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT 121 GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA 181 GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC 241 GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT 301 ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG 361 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG 421 CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT 481 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG 541 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC 601 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA 661 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT 721 GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA 781 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG 841 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG 901 TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT 961 TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG 1021 GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA 1081 TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA 1141 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA 1201 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC 1261 TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG 1321 CGGCCGCCCC CTTCACCATG GGCCACCATG GAAGGGACAG AAGCCCGACA GAGGAGGCTG 1381 GAAGGCTGTG GAAGGCTGAA GGAGCTGGGG CCATTGCCCA GCCACGATGC AGGAAGACTC 1441 CCAAAGGCTT CTGAAGAAGG ACACCTGGCG GTCTCTGAGA GCCAACTGGT GGATGCCAAA 1501 TCACTGGAGG CCCCGCCTGG CAGGGAGACG TCCCTGATCA TTGGCTTTCA GGTGGTAATA 1561 CCCTTCCTGC TTGCAGGCAT GGGACTGTCC TGGGCTGGCT TGCTTCTGAA CTATTTCCAG 1621 CACTGGCCAG TGTTCAAAGA TGTTAAAGAC CTGATGACGT TGGTGCCACC CCTCGTCGGT 1681 CTGAAGGGAA ACCTGGAAAT GACGCTGGCG TCTCGGCTCT CCACGTCTGC CAACACTGGG 1741 CAAATCGATG ACCGCCAGGA GAGGTACAAA ATCATCAGCA GCAACCTCGC TGTGGTTCAG 1801 GTGCAGGCCA CTGTTGTGGG GCTCCTGGCT GCAGTGGCCT CCCTGATGCT GGGCACAGTG 1861 TCCCACGAGG AGTTCGATTG GTCCAAGGTG GCCTTGCTGT GCACGAGCAG CGTCATCACC 1921 GCCTTCCTTG CTGCCTTGGC CCTAGGAATT CTAATGATCT GCATAGTGAT TGGTGCTCGG 1981 AAGTTTGGGG TCAACCCTGA TAACATCGCC ACACCCATTG CAGCCAGCCT GGGCGACCTC 2041 ATCACCCTGT CCATCCTGGC CCTCATGAGC AGTTTCTTCT ACAGCCACAA AGACACATGG 2101 TATCTGACGC CTCTGGTCTG TGTCGGCTTC TTGGCTCTCA CCCCTCTGTG GCTCTTCATT 2161 GCCAAGCAGA ACCCGCCCAT CATGAAGATC CTGAAGTATG GGTGGTTTCC CATCATCCTG 2221 GCTATGATCA TCAGCAGTTT CGGAGGTCTC ATCCTGAGCA AAACCATTTC GAAACATGAA 2281 TTTAAAGGCA TGGCTGTCCT GACTCCAGTC ATGTGTGGTG TTGGTGGCAA CCTGGTGGCC 2341 ATTCAGACTA GCCGCATCTC CACCTTTCTG CATATGTGGA GCACACCCGG GGTCCTCCCT 2401 GTCCAGATGA AAAGATTCTG GCCTAACCCC TGCTTCATTT TCTGCTCTTC AGAAATCAAT 2461 TCTGTGTCAG CACGAGTCCT GCTCTTTCTG GTGGTCCCAG GACACCTGAT TTTCTTCTAC 2521 CTCATCTGCC TGGTGGAAGG CCAGTCAGTG ACAAACAGCA AGATCTTCAT ATTGCTGTAC 2581 CTGGTGGCAG GCGTGGTCCA GGTGGTCATC CTGCTGTACC TGGCAGAAGT GACAGTTCGG 2641 CTGACATGGC ACCAAGCCCT GGATCCTGAC AACCACTGCA TCCCCTACCT CACGGGGCTG 2701 GGGGACCTGC TGGGCACTAG CCTGCTGGCA CTGTGCTTCT TCCTGGACTG GTTACTGAGG 2761 GGCAGAGCTA ATCTTCAGGA GCTGGTCTCA GAGCTGGTTT CTGTGCCTCC CTGATTTAAA 2821 CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC 2881 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG 2941 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG 3001 ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA 3061 TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA 3121 GCGGCGCATT AAATAACTTC GTATAATGTA TGCTATACGA AGTTATATCG TATGCATGCT 3181 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 3241 CCACAGAATT CCTTGAAATA CATCCACAGG TACACCTCCA ATTGACTCAA ATGATGTCAA 3301 TTAGTCTATC AGAAGCTTCT AAAGCCATGA CATCATTTTC TGGAATTTTC CAAGCTGTTT 3361 AAAGGCACAG TCAACTTAGT GTATGTAAAC TTCTGACCCA CTGGAATTGT GATACAGTGA 3421 ATTATAAGTG AAATAATCTG TCTGTAAACA ATTGTTGGAA AAATGACTTG TGTCATGCAC 3481 AAAGTAGATG TCCTAACTGA CTTGCCAAAA CTATTGTTTG TTAACAAGAA ATTTGTGGAG 3541 TAGTTGAAAA ACGAGTTTTA ATGACTCCAA CTTAAGTGTA TGTAAACTTC CGACTTCAAC 3601 TGTAGGGTAC CGAGCTCGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 3661 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 3721 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 3781 CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC 3841 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA 3901 CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG 3961 ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA 4021 CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT 4081 TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 4141 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 4201 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 4261 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT 4321 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 4381 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 4441 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 4501 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 4561 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC 4621 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 4681 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 4741 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 4801 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 4861 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA 4921 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 4981 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 5041 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 5101 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 5161 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 5221 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 5281 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 5341 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT 5401 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 5461 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 5521 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 5581 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 5641 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 5701 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 5761 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 5821 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 5881 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 5941 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA 6001 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG 6061 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC 6121 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG 6181 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC 6241 CATGATTACG CCAAGCTTGC ATGCCTGCAG GTCGACTCTA GACCTATACA GTTGAAGTCG 6301 GAAGTTTACA TACACTTAAG TTGGAGTCAT TAAAACTCGT TTTTCAACTA CTCCACAAAT 6361 TTCTTGTTAA CAAACAATAG TTTTGGCAAG TCAGTTAGGA CATCTACTTT GTGCATGACA 6421 CAAGTCATTT TTCCAACAAT TGTTTACAGA CAGATTATTT CACTTATAAT TCACTGTATC 6481 ACAATTCCAG TGGGTCAGAA GTTTACATAC ACTAAGTTGA CTGTGCCTTT AAACAGCTTG 6541 GAAAATTCCA GAAAATGATG TCATGGCTTT AGAAGCTTCT GATAGACTAA TTGACATCAT 6601 TTGAGTCAAT TGGAGGTGTA CCTGTGGATG TATTTCAAGG GGATCCAGAC ATGATAAGAT 6661 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGCG GCCGCTAGCT CGAGATCTTA 6721 ATTAAACTAG TATCGATATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TATGTACGGG 6781 CCAGATATAC GCGTTGACAT TGATTATTGA CTAGGCTTTT GCAAAAAGCT TTGCAAAGAT 6841 GGATAAAGTT TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG GTCTTGAAAG 6901 GAGTGGGAAT T

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.