pT3-EF1a-MYC-U6-Slc41a3-shRNA1

pT3-EF1a-MYC-U6-Slc41a3-shRNA1-IRES-luc plasmid

PVT96601

pT3-EF1a-MYC-U6-Slc41a3-shRNA1-IRES-luc plasmid Information

Plasmid name: pT3-EF1a-MYC-U6-Slc41a3(mouse)-shRNA1-IRES-luc
Alias: GCTGGCTTGCTTCTGAACTAT
Gene lenght:
Host: Mammalian cells
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: U6
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pT3-EF1a-MYC-U6-Slc41a3-shRNA1-IRES-luc plasmid Description

pT3-EF1a-MYC-U6-Slc41a3-shRNA1-IRES-luc This is a plasmid suitable for interference silencing in mammalian cells.

pT3-EF1a-MYC-U6-Slc41a3-shRNA1-IRES-luc plasmid Sequence

1 CTAGCTCGAG ATCTTAATTA AACTAGTATC GATATAACTT CGTATAATGT ATGCTATACG 61 AAGTTATATG TACGGGCCAG ATATACGCGT TATCGATCAC GAGACTAGCC TCGAGCGGCC 121 GCCCCCTTCA CCGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 181 GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 241 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 301 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 361 TTGTGGAAAG GACGAAACAC CGGGCTGGCT TGCTTCTGAA CTATTTCAAG AGAATAGTTC 421 AGAAGCAAGC CAGCTTTTTT GAATTCTCGA CCTCGAGACA AATGGCAGTA TTCATCTGAC 481 ATTGATTATT GACTAGGCTT TTGCAAAAAG CTTTGCAAAG ATGGATAAAG TTTTAAACAG 541 AGAGGAATCT TTGCAGCTAA TGGACCTTCT AGGTCTTGAA AGGAGTGGGA ATTGGCTCCG 601 GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC CGAGAAGTTG GGGGGAGGGG 661 TCGGCAATTG AACCGGTGCC TAGAGAAGGT GGCGCGGGGT AAACTGGGAA AGTGATGTCG 721 TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC GTATATAAGT GCAGTAGTCG 781 CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC ACAGGTAAGT GCCGTGTGTG 841 GTTCCCGCGG GCCTGGCCTC TTTACGGGTT ATGGCCCTTG CGTGCCTTGA ATTACTTCCA 901 CCTGGCTGCA GTACGTGATT CTTGATCCCG AGCTTCGGGT TGGAAGTGGG TGGGAGAGTT 961 CGAGGCCTTG CGCTTAAGGA GCCCCTTCGC CTCGTGCTTG AGTTGAGGCC TGGCCTGGGC 1021 GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC ACCTTCGCGC CTGTCTCGCT GCTTTCGATA 1081 AGTCTCTAGC CATTTAAAAT TTTTGATGAC CTGCTGCGAC GCTTTTTTTC TGGCAAGATA 1141 GTCTTGTAAA TGCGGGCCAA GATCTGCACA CTGGTATTTC GGTTTTTGGG GCCGCGGGCG 1201 GCGACGGGGC CCGTGCGTCC CAGCGCACAT GTTCGGCGAG GCGGGGCCTG CGAGCGCGGC 1261 CACCGAGAAT CGGACGGGGG TAGTCTCAAG CTGGCCGGCC TGCTCTGGTG CCTGGCCTCG 1321 CGCCGCCGTG TATCGCCCCG CCCTGGGCGG CAAGGCTGGC CCGGTCGGCA CCAGTTGCGT 1381 GAGCGGAAAG ATGGCCGCTT CCCGGCCCTG CTGCAGGGAG CTCAAAATGG AGGACGCGGC 1441 GCTCGGGAGA GCGGGCGGGT GAGTCACCCA CACAAAGGAA AAGGGCCTTT CCGTCCTCAG 1501 CCGTCGCTTC ATGTGACTCC ACGGAGTACC GGGCGCCGTC CAGGCACCTC GATTAGTTCT 1561 CGAGCTTTTG GAGTACGTCG TCTTTAGGTT GGGGGGAGGG GTTTTATGCG ATGGAGTTTC 1621 CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG GCACTTGATG TAATTCTCCT 1681 TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC 1741 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTAG CTTGGTACTA ATACGACTCA 1801 CTATAGGGAG ACCCAAGCTG GCTAGGTAAG CTTGGTACCG AGCTCGGATC CACTAGTCCA 1861 GTGTGGTGGA ATTCTGCAGA TATCAACAAG TTTGTACAAA AAAGCAGGCT TCGCCACCAT 1921 GCCCCTCAAC GTTAGCTTCA CCAACAGGAA CTATGACCTC GACTACGACT CGGTGCAGCC 1981 GTATTTCTAC TGCGACGAGG AGGAGAACTT CTACCAGCAG CAGCAGCAGA GCGAGCTGCA 2041 GCCCCCGGCG CCCAGCGAGG ATATCTGGAA GAAATTCGAG CTGCTGCCCA CCCCGCCCCT 2101 GTCCCCTAGC CGCCGCTCCG GGCTCTGCTC GCCCTCCTAC GTTGCGGTCA CACCCTTCTC 2161 CCTTCGGGGA GACAACGACG GCGGTGGCGG GAGCTTCTCC ACGGCCGACC AGCTGGAGAT 2221 GGTGACCGAG CTGCTGGGAG GAGACATGGT GAACCAGAGT TTCATCTGCG ACCCGGACGA 2281 CGAGACCTTC ATCAAAAACA TCATCATCCA GGACTGTATG TGGAGCGGCT TCTCGGCCGC 2341 CGCCAAGCTC GTCTCAGAGA AGCTGGCCTC CTACCAGGCT GCGCGCAAAG ACAGCGGCAG 2401 CCCGAACCCC GCCCGCGGCC ACAGCGTCTG CTCCACCTCC AGCTTGTACC TGCAGGATCT 2461 GAGCGCCGCC GCCTCAGAGT GCATCGACCC CTCGGTGGTC TTCCCCTACC CTCTCAACGA 2521 CAGCAGCTCG CCCAAGTCCT GCGCCTCGCA AGACTCCAGC GCCTTCTCTC CGTCCTCGGA 2581 TTCTCTGCTC TCCTCGACGG AGTCCTCCCC GCAGGGCAGC CCCGAGCCCC TGGTGCTCCA 2641 TGAGGAGACA CCGCCCACCA CCAGCAGCGA CTCTGAGGAG GAACAAGAAG ATGAGGAAGA 2701 AATCGATGTT GTTTCTGTGG AAAAGAGGCA GGCTCCTGGC AAAAGGTCAG AGTCTGGATC 2761 ACCTTCTGCT GGAGGCCACA GCAAACCTCC TCACAGCCCA CTGGTCCTCA AGAGGTGCCA 2821 CGTCTCCACA CATCAGCACA ACTACGCAGC GCCTCCCTCC ACTCGGAAGG ACTATCCTGC 2881 TGCCAAGAGG GTCAAGTTGG ACAGTGTCAG AGTCCTGAGA CAGATCAGCA ACAACCGAAA 2941 ATGCACCAGC CCCAGGTCCT CGGACACCGA GGAGAATGTC AAGAGGCGAA CACACAACGT 3001 CTTGGAGCGC CAGAGGAGGA ACGAGCTAAA ACGGAGCTTT TTTGCCCTGC GTGACCAGAT 3061 CCCGGAGTTG GAAAACAATG AAAAGGCCCC CAAGGTAGTT ATCCTTAAAA AAGCCACAGC 3121 ATACATCCTG TCCGTCCAAG CAGAGGAGCA AAAGCTCATT TCTGAAGAGG ACTTGTTGCG 3181 GAAACGACGA GAACAGTTGA AACACAAACT TGAACAGCTA CGGAACTCTT GTGCGGACCC 3241 AGCTTTCTTG TACAAAGTGG TTGATATCCA GCACAGTGGC GGCCGCTCGA GTCTAGAGGG 3301 CCCGCGGTTC GAAGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGCGTAC 3361 CGGTTAGTAA TGAGTTTCCC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG 3421 GCCGGTGTGC GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA 3481 GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG 3541 CCAAAGGAAT GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT 3601 GAAGACAAAC AACGTCTGTA GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA 3661 GGTGCCTCTG CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC 3721 AGTGCCACGT TGTGAGTTGG ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT 3781 TCAACAAGGG GCTGAAGGAT GCCCAGAAGG TACCCCATTG TATGGGATCT GATCTGGGGC 3841 CTCGGTGCAC ATGCTTTACA TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA 3901 ACCACGGGGA CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGAA 3961 GACGCCAAAA ACATAAAGAA AGGCCCGGCG CCATTCTATC CGCTGGAAGA TGGAACCGCT 4021 GGAGAGCAAC TGCATAAGGC TATGAAGAGA TACGCCCTGG TTCCTGGAAC AATTGCTTTT 4081 ACAGATGCAC ATATCGAGGT GGACATCACT TACGCTGAGT ACTTCGAAAT GTCCGTTCGG 4141 TTGGCAGAAG CTATGAAACG ATATGGGCTG AATACAAATC ACAGAATCGT CGTATGCAGT 4201 GAAAACTCTC TTCAATTCTT TATGCCGGTG TTGGGCGCGT TATTTATCGG AGTTGCAGTT 4261 GCGCCCGCGA ACGACATTTA TAATGAACGT GAATTGCTCA ACAGTATGGG CATTTCGCAG 4321 CCTACCGTGG TGTTCGTTTC CAAAAAGGGG TTGCAAAAAA TTTTGAACGT GCAAAAAAAG 4381 CTCCCAATCA TCCAAAAAAT TATTATCATG GATTCTAAAA CGGATTACCA GGGATTTCAG 4441 TCGATGTACA CGTTCGTCAC ATCTCATCTA CCTCCCGGTT TTAATGAATA CGATTTTGTG 4501 CCAGAGTCCT TCGATAGGGA CAAGACAATT GCACTGATCA TGAACTCCTC TGGATCTACT 4561 GGTCTGCCTA AAGGTGTCGC TCTGCCTCAT AGAACTGCCT GCGTGAGATT CTCGCATGCC 4621 AGAGATCCTA TTTTTGGCAA TCAAATCATT CCGGATACTG CGATTTTAAG TGTTGTTCCA 4681 TTCCATCACG GTTTTGGAAT GTTTACTACA CTCGGATATT TGATATGTGG ATTTCGAGTC 4741 GTCTTAATGT ATAGATTTGA AGAAGAGCTG TTTCTGAGGA GCCTTCAGGA TTACAAGATT 4801 CAAAGTGCGC TGCTGGTGCC AACCCTATTC TCCTTCTTCG CCAAAAGCAC TCTGATTGAC 4861 AAATACGATT TATCTAATTT ACACGAAATT GCTTCTGGTG GCGCTCCCCT CTCTAAGGAA 4921 GTCGGGGAAG CGGTTGCCAA GAGGTTCCAT CTGCCAGGTA TCAGGCAAGG ATATGGGCTC 4981 ACTGAGACTA CATCAGCTAT TCTGATTACA CCCGAGGGGG ATGATAAACC GGGCGCGGTC 5041 GGTAAAGTTG TTCCATTTTT TGAAGCGAAG GTTGTGGATC TGGATACCGG GAAAACGCTG 5101 GGCGTTAATC AAAGAGGCGA ACTGTGTGTG AGAGGTCCTA TGATTATGTC CGGTTATGTA 5161 AACAATCCGG AAGCGACCAA CGCCTTGATT GACAAGGATG GATGGCTACA TTCTGGAGAC 5221 ATAGCTTACT GGGACGAAGA CGAACACTTC TTCATCGTTG ACCGCCTGAA GTCTCTGATT 5281 AAGTACAAAG GCTATCAGGT GGCTCCCGCT GAATTGGAAT CCATCTTGCT CCAACACCCC 5341 AACATCTTCG ACGCAGGTGT CGCAGGTCTT CCCGACGATG ACGCCGGTGA ACTTCCCGCC 5401 GCCGTTGTTG TTTTGGAGCA CGGAAAGACG ATGACGGAAA AAGAGATCGT GGATTACGTC 5461 GCCAGTCAAG TAACAACCGC GAAAAAGTTG CGCGGAGGAG TTGTGTTTGT GGACGAAGTA 5521 CCGAAAGGTC TTACCGGAAA ACTCGACGCA AGAAAAATCA GAGAGATCCT CATAAAGGCC 5581 AAGAAGGGCG GAAAGATCGC CGTGTAATTC TAGGATCCGT CGACCTGCAG CCAAGCTTAT 5641 CGATAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC 5701 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA 5761 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG 5821 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG 5881 GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG 5941 CCCTGTAGCG GCGCATTAAA TAACTTCGTA TAATGTATGC TATACGAAGT TATATCGTAT 6001 GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC 6061 ACACATTCCA CAGAATTCCT TGAAATACAT CCACAGGTAC ACCTCCAATT GACTCAAATG 6121 ATGTCAATTA GTCTATCAGA AGCTTCTAAA GCCATGACAT CATTTTCTGG AATTTTCCAA 6181 GCTGTTTAAA GGCACAGTCA ACTTAGTGTA TGTAAACTTC TGACCCACTG GAATTGTGAT 6241 ACAGTGAATT ATAAGTGAAA TAATCTGTCT GTAAACAATT GTTGGAAAAA TGACTTGTGT 6301 CATGCACAAA GTAGATGTCC TAACTGACTT GCCAAAACTA TTGTTTGTTA ACAAGAAATT 6361 TGTGGAGTAG TTGAAAAACG AGTTTTAATG ACTCCAACTT AAGTGTATGT AAACTTCCGA 6421 CTTCAACTGT AGGGTACCGA GCTCGAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG 6481 GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG 6541 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG 6601 CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT 6661 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC 6721 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA 6781 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG 6841 CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT 6901 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT 6961 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT 7021 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC 7081 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA 7141 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG 7201 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT 7261 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG 7321 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC 7381 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC 7441 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA 7501 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC 7561 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT 7621 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA 7681 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 7741 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA 7801 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA 7861 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT 7921 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT 7981 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG 8041 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 8101 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 8161 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 8221 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 8281 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 8341 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 8401 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 8461 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 8521 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 8581 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 8641 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 8701 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA 8761 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG 8821 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG 8881 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA 8941 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT 9001 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG 9061 CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC GACTCTAGAC CTATACAGTT 9121 GAAGTCGGAA GTTTACATAC ACTTAAGTTG GAGTCATTAA AACTCGTTTT TCAACTACTC 9181 CACAAATTTC TTGTTAACAA ACAATAGTTT TGGCAAGTCA GTTAGGACAT CTACTTTGTG 9241 CATGACACAA GTCATTTTTC CAACAATTGT TTACAGACAG ATTATTTCAC TTATAATTCA 9301 CTGTATCACA ATTCCAGTGG GTCAGAAGTT TACATACACT AAGTTGACTG TGCCTTTAAA 9361 CAGCTTGGAA AATTCCAGAA AATGATGTCA TGGCTTTAGA AGCTTCTGAT AGACTAATTG 9421 ACATCATTTG AGTCAATTGG AGGTGTACCT GTGGATGTAT TTCAAGGGGA TCCAGACATG 9481 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGCGGCC G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.