pLV3-CMV-XPO1-3×HA-EF1a-mCherr

pLV3-CMV-XPO1-3×HA-EF1a-mCherry-Puro plasmid

PVT96226

pLV3-CMV-XPO1-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-XPO1(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_003400.4;CRM-1; CRM1; emb; exp1
Gene lenght: 3213bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-XPO1-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-XPO1-3×HA-EF1a-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-XPO1-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgccagc aattatgaca atgttagcag accatgcagc 2341 tcgtcagctg cttgatttca gccaaaaact ggatatcaac ttattagata atgtggtgaa 2401 ttgcttatac catggagaag gagcccagca aagaatggct caagaagtac tgacacattt 2461 aaaggagcat cctgatgctt ggacaagagt cgacacaatt ttggaatttt ctcagaatat 2521 gaatacgaaa tactatggac tacaaatttt ggaaaatgtg ataaaaacaa ggtggaagat 2581 tcttccaagg aaccagtgcg aaggaataaa aaaatacgtt gttggcctca ttatcaagac 2641 gtcatctgac ccaacttgtg tagagaaaga aaaggtgtat atcggaaaat taaatatgat 2701 ccttgttcag atactgaaac aagaatggcc caaacattgg ccaactttta tcagtgatat 2761 tgttggagca agtaggacca gcgaaagtct ctgtcaaaat aatatggtga ttcttaaact 2821 cttgagtgaa gaagtatttg atttctctag tggacagata acccaagtca aatctaagca 2881 tttaaaagac agcatgtgca atgaattctc acagatattt caactgtgtc agtttgtaat 2941 ggaaaattct caaaatgctc cacttgtaca tgcaaccttg gaaacattgc tcagatttct 3001 gaactggatt cccctgggat atatttttga gaccaaatta atcagcacat tgatttataa 3061 gttcctgaat gttccaatgt ttcgaaatgt ctctctgaag tgcctcactg agattgctgg 3121 tgtgagtgta agccaatatg aagaacaatt tgtaacacta tttactctga caatgatgca 3181 actaaagcag atgcttcctt taaataccaa tattcgactt gcgtactcaa atggaaaaga 3241 tgatgaacag aacttcattc aaaatctcag tttgtttctc tgcacctttc ttaaggaaca 3301 tgatcaactt atagaaaaaa gattaaatct cagggaaact cttatggagg cccttcatta 3361 tatgttgttg gtatctgaag tagaagaaac tgaaatcttt aaaatttgtc ttgaatactg 3421 gaatcatttg gctgctgaac tctatagaga gagtccattc tctacatctg cctctccgtt 3481 gctttctgga agtcaacatt ttgatgttcc tcccaggaga cagctatatt tgcccatgtt 3541 attcaaggtc cgtttattaa tggttagtcg aatggctaaa ccagaggaag tattggttgt 3601 agagaatgat caaggagaag ttgtgagaga attcatgaag gatacagatt ccataaattt 3661 gtataagaat atgagggaaa cattggttta tcttactcat ctggattatg tagatacaga 3721 aagaataatg acagagaagc ttcacaatca agtgaatggt acagagtggt catggaaaaa 3781 tttgaataca ttgtgttggg caataggctc cattagtgga gcaatgcatg aagaggacga 3841 aaaacgattt cttgttactg ttataaagga tctattagga ttatgtgaac agaaaagagg 3901 caaagataat aaagctatta ttgcatcaaa tatcatgtac atagtaggtc aatacccacg 3961 ttttttgaga gctcactgga aatttctgaa gactgtagtt aacaagctgt tcgaattcat 4021 gcatgagacc catgatggag tccaggatat ggcttgtgat actttcatta aaatagccca 4081 aaaatgccgc aggcatttcg ttcaggttca ggttggagaa gtgatgccat ttattgatga 4141 aattttgaac aacattaaca ctattatttg tgatcttcag cctcaacagg ttcatacgtt 4201 ttatgaagct gtggggtaca tgattggtgc acaaacagat caaacagtac aagaacactt 4261 gatagaaaag tacatgttac tccctaatca agtgtgggat agtataatcc agcaggcaac 4321 caaaaatgtg gatatactga aagatcctga aacagtcaag cagcttggta gcattttgaa 4381 aacaaatgtg agagcctgca aagctgttgg acaccccttt gtaattcagc ttggaagaat 4441 ttatttagat atgcttaatg tatacaagtg cctcagtgaa aatatttctg cagctatcca 4501 agctaatggt gaaatggtta caaagcaacc attgattaga agtatgcgaa ctgtaaaaag 4561 ggaaacttta aagttaatat ctggttgggt gagccgatcc aatgatccac agatggtcgc 4621 tgaaaatttt gttccccctc tgttggatgc agttctcatt gattatcaga gaaatgtccc 4681 agctgctaga gaaccagaag tgcttagtac tatggccata attgtcaaca agttaggggg 4741 acatataaca gctgaaatac ctcaaatatt tgatgctgtt tttgaatgca cattgaatat 4801 gataaataag gactttgaag aatatcctga acatagaacg aactttttct tactacttca 4861 ggctgtcaat tctcattgtt tcccagcatt ccttgctatt ccacctacac agtttaaact 4921 tgttttggat tccatcattt gggctttcaa acatactatg aggaatgtcg cagatacggg 4981 cttacagata ctttttacac tcttacaaaa tgttgcacaa gaagaagctg cagctcagag 5041 tttttatcaa acttattttt gtgatattct ccagcatatc ttttctgttg tgacagacac 5101 ttcacatact gctggtttaa caatgcatgc atcaattctt gcatatatgt ttaatttggt 5161 tgaagaagga aaaataagta catcattaaa tcctggaaat ccagttaaca accaaatctt 5221 tcttcaggaa tatgtggcta atctccttaa gtcggccttc cctcacctac aagatgctca 5281 agtaaagctc tttgtgacag ggcttttcag cttaaatcaa gatattcctg ctttcaagga 5341 acatttaaga gatttcctag ttcaaataaa ggaatttgca ggtgaagaca cttctgattt 5401 gtttttggaa gagagagaaa tagccctacg gcaggctgat gaagagaaac ataaacgtca 5461 aatgtctgtc cctggcatct ttaatccaca tgagattcca gaagaaatgt gtgatggatc 5521 cTACCCATAC GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG 5581 CTATCCTTAT GATGTCCCAG ACTACGCATA GgcggccgcG aaggatctgc gatcgctccg 5641 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg 5701 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg 5761 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg 5821 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg 5881 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga 5941 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa 6001 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac 6061 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt 6121 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca 6181 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg 6241 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc 6301 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct 6361 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc 6421 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg 6481 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg 6541 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa 6601 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg 6661 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg 6721 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca 6781 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac 6841 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga tctcgcgagg 6901 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgaccgagt 6961 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 7021 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 7081 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 7141 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 7201 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 7261 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 7321 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 7381 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 7441 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 7501 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga aatcaacctc 7561 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 7621 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 7681 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 7741 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 7801 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 7861 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 7921 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 7981 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 8041 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc 8101 ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta agaccaatga 8161 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 8221 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag 8281 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 8341 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 8401 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 8461 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 8521 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 8581 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 8641 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 8701 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 8761 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc 8821 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 8881 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 8941 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 9001 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 9061 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 9121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 9181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 9241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 9301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 9361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 9421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 9481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 9541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 9601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 9661 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 9721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 9781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 9841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 9901 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 9961 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 10021 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 10081 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 10141 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 10201 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 10261 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 10321 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 10381 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 10441 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 10501 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 10561 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 10621 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 10681 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 10741 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 10801 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 10861 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 10921 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 10981 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 11041 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 11101 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 11161 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 11221 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 11281 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 11341 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 11401 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.