pLV3-CMV-METTL3-3×HA-EF1a-mChe

pLV3-CMV-METTL3-3×HA-EF1a-mCherry-Puro plasmid

PVT94071

pLV3-CMV-METTL3-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-METTL3(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_019852.5;hMETTL3; IME4; M6A; MT-A70; Spo8;
Gene lenght: 1740bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-METTL3-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-METTL3-3×HA-EF1a-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-METTL3-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgtcgga cacgtggagc tctatccagg cccacaagaa 2341 gcagctggac tctctgcggg agaggctgca gcggaggcgg aagcaggact cggggcactt 2401 ggatctacgg aatccagagg cagcattgtc tccaaccttc cgtagtgaca gcccagtgcc 2461 tactgcaccc acctctggtg gccctaagcc cagcacagct tcagcagttc ctgaattagc 2521 tacagatcct gagttagaga agaagttgct acaccacctc tctgatctgg ccttaacatt 2581 gcccactgat gctgtgtcca tctgtcttgc catctccacg ccagatgctc ctgccactca 2641 agatggggta gaaagcctcc tgcagaagtt tgcagctcag gagttgattg aggtaaagcg 2701 aggtctccta caagatgatg cacatcctac tcttgtaacc tatgctgacc attccaagct 2761 ctctgccatg atgggtgctg tggcagaaaa gaagggccct ggggaggtag cagggactgt 2821 cacagggcag aagcggcgtg cagaacagga ctcgactaca gtagctgcct ttgccagttc 2881 gttagtctct ggtctgaact cttcagcatc ggaaccagca aaggagccag ccaagaaatc 2941 aaggaaacat gctgcctcag atgttgatct ggagatagag agccttctga accaacagtc 3001 cactaaggaa caacagagca agaaggtcag tcaggagatc ctagagctat taaatactac 3061 aacagccaag gaacaatcca ttgttgaaaa atttcgctct cgaggtcggg cccaagtgca 3121 agaattctgt gactatggaa ccaaggagga gtgcatgaaa gccagtgatg ctgatcgacc 3181 ctgtcgcaag ctgcacttca gacgaattat caataaacac actgatgagt ctttaggtga 3241 ctgctctttc cttaatacat gtttccacat ggatacctgc aagtatgttc actatgaaat 3301 tgatgcttgc atggattctg aggcccctgg cagcaaagac cacacgccaa gccaggagct 3361 tgctcttaca cagagtgtcg gaggtgattc cagtgcagac cgactcttcc cacctcagtg 3421 gatctgttgt gatatccgct acctggacgt cagtatcttg ggcaagtttg cagttgtgat 3481 ggctgaccca ccctgggata ttcacatgga actgccctat gggaccctga cagatgatga 3541 gatgcgcagg ctcaacatac ccgtactaca ggatgatggc tttctcttcc tctgggtcac 3601 aggcagggcc atggagttgg ggagagaatg tctaaacctc tgggggtatg aacgggtaga 3661 tgaaattatt tgggtgaaga caaatcaact gcaacgcatc attcggacag gccgtacagg 3721 tcactggttg aaccatggga aggaacactg cttggttggt gtcaaaggaa atccccaagg 3781 cttcaaccag ggtctggatt gtgatgtgat cgtagctgag gttcgttcca ccagtcataa 3841 accagatgaa atctatggca tgattgaaag actatctcct ggcactcgca agattgagtt 3901 atttggacga ccacacaatg tgcaacccaa ctggatcacc cttggaaacc aactggatgg 3961 gatccaccta ctagacccag atgtggttgc acggttcaag caaaggtacc cagatggtat 4021 catctctaaa cctaagaatt taggatccTA CCCATACGAC GTACCTGATT ATGCAACTTA 4081 TCCTTATGAT GTACCTGATT ATGCTAGCTA TCCTTATGAT GTCCCAGACT ACGCATAGgc 4141 ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 4201 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 4261 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 4321 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 4381 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 4441 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 4501 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 4561 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 4621 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 4681 gaccggcgcc tacgctagac gccaccatgg tgagcaaggg cgaggaggat aacatggcca 4741 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 4801 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 4861 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 4921 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 4981 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 5041 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca 5101 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct 5161 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 5221 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 5281 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg 5341 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg 5401 acgagctgta caagggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg 5461 aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 5521 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 5581 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 5641 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 5701 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 5761 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 5821 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 5881 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 5941 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 6001 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 6061 gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 6121 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 6181 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 6241 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 6301 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 6361 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 6421 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 6481 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 6541 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 6601 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc 6661 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 6721 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt 6781 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 6841 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 6901 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 6961 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 7021 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 7081 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 7141 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 7201 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 7261 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 7321 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa 7381 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7441 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7501 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7561 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7621 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7681 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 7741 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 7801 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7861 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7921 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7981 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 8041 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 8101 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 8161 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 8221 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8281 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8341 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8401 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8461 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8521 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 8581 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8641 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8701 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8761 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8821 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8881 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8941 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 9001 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 9061 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 9121 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9181 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9241 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9301 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9361 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9421 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9481 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 9541 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 9601 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 9661 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 9721 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 9781 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 9841 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 9901 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 9961 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.