pCMV-3×FLAG-TNIP1-Neo plasmid

pCMV-3×FLAG-TNIP1-Neo plasmid

PVT96221

pCMV-3×FLAG-TNIP1-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-TNIP1(human)-Neo
Alias: NM_001252385.2;ABIN-1; NAF1; nip40-1; VAN
Gene lenght: 1860bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-TNIP1-Neo plasmid Description

pCMV-3×FLAG-TNIP1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-TNIP1-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggaaggga gaggaccgta ccggatctac 1021 gaccctgggg gcagcgtgcc ctcaggagag gcatccgcag cttttgagcg cctagtgaag 1081 gagaattccc ggctgaagga aaaaatgcaa gggataaaga tgttagggga gcttttggaa 1141 gagtcccaga tggaagcgac caggctccgg cagaaggcag aggagctagt gaaggacaac 1201 gagctgctcc caccaccttc tccctccttg ggctccttcg accccctggc tgagctcaca 1261 ggaaaggact caaatgtcac agcatctccc acagcccctg catgccccag tgacaagcca 1321 gcaccagtcc agaagcctcc atccagtggc acctcctctg aatttgaagt ggtcactcct 1381 gaggagcaga attcaccaga gagcagcagc catgccaatg cgatggcgct gggccccctg 1441 ccccgtgagg acggcaacct gatgctgcac ctgcagcgcc tggagaccac gctgagtgtg 1501 tgtgccgagg agccggacca cggccagctc ttcacccacc tgggccgcat ggccctggag 1561 ttcaaccgac tggcatccaa ggtgcacaag aatgagcagc gcacctccat tctgcagacc 1621 ctgtgtgagc agcttcggaa ggagaacgag gctctgaagg ccaagttgga taagggcctg 1681 gaacagcggg atcaggctgc cgagaggctg cgggaggaaa atttggagct caagaagttg 1741 ttgatgagca atggcaacaa agagggtgcg tctgggcggc caggctcacc gaagatggaa 1801 gggacaggca agaaggcagt ggctggacag cagcaggcta gtgtgacggc aggtaaggtc 1861 ccagaggtgg tggccttggg cgcagccgag aagaaggtga agatgctgga gcagcagcgc 1921 agtgagctgc tggaagtgaa caagcagtgg gaccagcatt tccggtccat gaagcagcag 1981 tatgagcaga agatcactga gctgcgtcag aagctggctg atttgcagaa gcaggtgact 2041 gacctggagg ccgagcggga gcagaagcag cgtgactttg accgcaagct cctcctggcc 2101 aagtccaaga ttgaaatgga ggagaccgac aaggagcagc tgacagcaga ggccaaggag 2161 ctgcgccaaa aggtcaagta cctgcaggat cagctgagcc cactcacccg acagcgtgag 2221 taccaggaaa aggagatcca gcggctcaac aaggccctgg aggaagcact gagcatccaa 2281 accccgccat catctccacc aacagcattt gggagcccag aaggagcagg ggccctccta 2341 aggaaacagg agctggtcac gcagaatgag ttgctgaaac agcaggtgaa gatcttcgag 2401 gaggacttcc agagggagcg cagtgatcgt gagcgcatga atgaggagaa ggaagagctg 2461 aagaagcaag tggagaagct gcaggcccag gtcaccctgt caaatgccca gctaaaagca 2521 ttcaaagatg aggagaaggc aagagaagcc ctcagacagc agaagaggaa agcaaaggcc 2581 tcaggagagc gttaccatgt ggagccccac ccagaacatc tctgcggggc ctacccctac 2641 gcctacccgc ccatgccagc catggtgcca caccatggct tcgaggactg gtcccagatc 2701 cgctaccccc ctccccccat ggccatggag cacccgcccc cactccccaa ctcgcgcctc 2761 ttccatctgc accagttttg tcgcagccgg aatacacctg gcgtctaccc tgtggagggg 2821 ttcgaaatcc aaatcagagc tcccaagtga gaattctgca gatatccagc acagtggcgg 2881 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt 2941 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 3001 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 3061 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 3121 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 3181 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 3241 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 3301 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 3361 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 3421 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 3481 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 3541 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 3601 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa 3661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3721 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3781 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3901 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 3961 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 4021 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 4081 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 4141 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 4201 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 4261 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 4321 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 4381 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 4441 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 4501 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 4561 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 4621 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 4681 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 4741 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 4801 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 4861 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 4921 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 4981 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 5041 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 5101 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 5161 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 5221 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 5281 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 5341 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 5401 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 5461 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 5521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 5581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 5641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 5701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 5761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 5821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 5881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 5941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6001 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt 6121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 6181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6241 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6301 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 6361 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 6421 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6481 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6541 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6601 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6661 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6721 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6781 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6841 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 6901 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 6961 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7021 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7081 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7141 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 7201 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 7261 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 7321 tgacgtc

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.