pLV3-CMV-SETD2-2-3×FLAG-Puro p

pLV3-CMV-SETD2-2-3×FLAG-Puro plasmid

PVT89594

pLV3-CMV-SETD2-2-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-SETD2(human)-2-3×FLAG-Puro
Alias:
Gene lenght: 7692bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-SETD2-2-3×FLAG-Puro plasmid Description

pLV3-CMV-SETD2-2-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SETD2-2-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgaagcag ctgcagccgc agccgcctcc gaagatgggg 2341 gatttctacg acccggagca cccgacccct gaagaagaag aaaatgaggc aaagattgaa 2401 aatgtgcaga aaacaggttt catcaaagga ccaatgttca aaggtgttgc ttctagtcga 2461 tttttgccca aaggcaccaa aacaaaagtt aatttggaag aacagggacg acagaaggtg 2521 tcattcagct tcagccttac aaagaaaact ttgcagaata ggtttctcac tgcacttggc 2581 aatgaaaagc aaagtgatac tccaaaccct ccagctgtac ctcttcaggt agactcgact 2641 cctaaaatga aaatggaaat tggtgatacc ttatctactg cagaagaatc ttccccacca 2701 aagtcaaggg tggaattggg caaaattcat tttaagaaac atctgcttca tgtaacatcc 2761 aggccactgc tggctactac cacagcagta gcatctccac ctactcatgc agcaccatta 2821 ccagcagtga tagcagaatc aacaactgta gactcaccgc cctcatctcc gcctccaccg 2881 cctccacctg cccaagccac aacactctca tcaccagcac cagtaacaga gccagtggcc 2941 ttgccacata caccaataac agttctaatg gcagcaccag tacccttacc agtagatgta 3001 gcagttagat ctctgaaaga accaccaatt ataattgtac cagaatcttt agaagcagat 3061 actaagcagg acactatatc taatagttta gaagaacacg taactcaaat attgaatgag 3121 caagcagata tttcctcaaa aaaagaagat tcccatattg ggaaggatga agaaattcca 3181 gatagttcta agattagtct gagctgtaaa aaaacaggtt ctaagaagaa atcctcacaa 3241 tctgaaggca tctttcttgg ttcagaatct gatgaagatt ctgtacggac ttcttcaagt 3301 caaagatcac atgatttaaa attttcagca agcattgaaa aggaaagaga ttttaaaaag 3361 agctcagcac ctttaaaaag tgaggatcta gggaaacctt cacgatctaa aacagacaga 3421 gatgataaat attttagcta ttcaaaactt gaaagagata ctcggtatgt atcttcccga 3481 tgtagatcag aaagagagcg acggcggagc agatctcact ctaggtctga gagaggctct 3541 agaactaatt tatcctattc caggtcagaa cgatctcatt attatgactc tgatcgtcgc 3601 taccatagga gctcccctta tcgagagagg acgcgctatt ctcggccata cacagataac 3661 agagcacgag agagttctga ctcagaagaa gagtataaga agacatactc aaggcgtacc 3721 tcatctcatt cctcttctta cagagaccta aggacatcat cctattctaa atctgatcgg 3781 gactgtaaaa ctgagacctc ttacttagag atggaaagaa gaggcaagta ttcttcaaaa 3841 ctagaaagag aatctaaaag gacttcagaa aatgaagcaa ttaaaagatg ttgttctccc 3901 cctaatgaac tgggattccg acgagggtca tcatattcta agcatgacag tagtgcttcc 3961 cgttataaat ctaccctttc aaaacctata cccaagtctg ataaatttaa aaattctttc 4021 tgttgtacag aattaaatga agaaatcaaa cagtctcatt cttttagttt acagacacct 4081 tgttcaaaag gtagtgaatt aagaatgatt aataaaaatc ctgaaagaga aaaggctggg 4141 tctccagctc catcaaatcg attaaatgat tcacctactt taaaaaagct agatgaattg 4201 cctattttta agtccgaatt tataacacat gatagccatg atagtattaa ggaattagac 4261 tctttatcta aagtgaagaa tgatcaatta agaagttttt gtcccataga attaaatata 4321 aatggatctc ctggggcaga atctgatttg gcaacatttt gcacttctaa aactgatgct 4381 gttttaatga cttctgatga tagtgtgact ggatcggaat tatccccttt ggtcaaagca 4441 tgcatgcttt catcaaatgg atttcagaat attagtaggt gcaaagaaaa agacttggat 4501 gatacctgca tgctgcataa gaagtcagaa agcccattta gagaaacaga acctctggtg 4561 tcaccacacc aagataaact catgtctatg ccagttatga ctgtggatta ttccaaaaca 4621 gtagttaaag aaccagttga tacgagggtt tcttgctgca aaaccaaaga ttcagacata 4681 tactgtactt tgaacgatag caacccttct ttgtgtaact ctgaagctga aaatattgag 4741 ccttcagtta tgaagatttc ttcaaatagc tttatgaatg tgcatttgga atcaaaacca 4801 gttatatgtg atagtagaaa tttgacagat cactcaaaat ttgcatgtga agaatataag 4861 cagagcatcg gtagcactag ttcagcttct gttaatcatt ttgatgattt atatcaacct 4921 attgggagtt caggtattgc ttcatctctt cagagtcttc caccaggaat aaaggtggac 4981 agtctaactc tcttgaaatg cggagagaac acatctccag ttctggatgc agtgctaaag 5041 agtaaaaaaa gttcagagtt tttaaagcat gcagggaaag aaacaatagt agaagtaggt 5101 agtgaccttc ctgattcagg aaagggattt gcttccaggg agaacaggcg taataatggg 5161 ttatctggga aatgtttgca agaggctcaa gaagaaggga attccatatt gcctgaaaga 5221 agaggaagac cagaaatctc tttagatgaa agaggagaag gaggacatgt gcatacttct 5281 gatgactcag aagttgtatt ttcttcttgt gatttgaatt taaccatgga agacagtgat 5341 ggtgtaactt atgcattaaa gtgtgacagt agtggtcatg ccccagaaat tgtgtctaca 5401 gttcatgaag attattctgg ctcttctgaa agttcaaatg atgaaagtga ttcagaagat 5461 acagattcgg atgatagcag tattccaaga aaccgtctcc agtctgttgt ggttgtgcca 5521 aagaattcta ctttgcccat ggaagaaaca agtccttgtt cttctcggag cagtcaaagt 5581 tatagacact attctgacca ttgggaagat gagagattgg agtcaaggag acatttgtat 5641 gaggaaaaat ttgaaagtat agcaagtaaa gcctgtcctc aaactgataa gtttttcctt 5701 cataaaggaa cagagaagaa tccggaaatt tcttttacac agtccagtag aaaacaaata 5761 gataatcgcc tgcctgaact ttctcatcct cagagtgatg gggttgatag tacaagtcat 5821 acagatgtga aatctgaccc tctgggtcac ccaaattcag aggaaaccgt gaaagccaaa 5881 ataccttcta ggcagcaaga agagctgcca atttattctt ctgattttga agatgtccca 5941 aataagtctt ggcaacagac cactttccaa aacaggccag atagtagact gggaaaaaca 6001 gaattgagtt tttcttcctc ttgtgagata ccacatgtgg atggcttgca ctcatcagaa 6061 gagctcagaa acttaggttg ggacttctct caagaaaagc cttctaccac gtatcagcaa 6121 cctgacagta gctatggagc ttgtggtgga cacaagtatc agcaaaatgc agaacagtat 6181 ggtgggacac gtgattactg gcaaggcaat ggttactggg atccaagatc aggtagacct 6241 cctggaactg gggttgtgta tgatcgaact caaggacaag taccagattc cctaacagat 6301 gatcgtgaag aagaggagaa ttgggatcaa caggatggat cccatttttc agaccagtcc 6361 gataaatttc ttctatccct tcagaaagac aaggggtcag tgcaagcacc tgaaataagc 6421 agcaattcca ttaaggacac tttagctgtg aatgaaaaga aagatttttc aaaaaactta 6481 gaaaaaaatg atatcaaaga tagagggcct cttaaaaaaa ggaggcagga aatagagagt 6541 gattctgaaa gtgatggtga gcttcaggac agaaagaaag ttagagtgga ggtagagcag 6601 ggagagacat cagtgccccc aggttcagca ctggttgggc cctcctgtgt catggatgac 6661 ttcagggacc cacagcgatg gaaggaatgt gccaagcaag ggaaaatgcc atgttacttt 6721 gatcttattg aagaaaatgt ttatttaaca gaaagaaaga agaataaatc tcatcgagat 6781 attaagcgaa tgcagtgtga gtgtacacct ctttctaaag atgaaagagc tcaaggtgaa 6841 atagcatgtg gggaagattg tcttaatcgt cttctcatga ttgaatgttc ttctcggtgt 6901 ccaaatgggg attattgttc caatagacgg tttcagagaa aacagcatgc agatgtggaa 6961 gtcatactca cagaaaagaa aggctggggc ttgagagctg ccaaagacct tccttcgaac 7021 acctttgtcc tagaatattg tggagaggta ctcgatcata aagagtttaa agctcgagtg 7081 aaggagtatg cacgaaacaa aaacatccat tactatttca tggccctgaa gaatgatgag 7141 ataatagatg ccactcaaaa aggaaattgc tctcgtttca tgaatcacag ctgtgaacca 7201 aattgtgaaa cccaaaaatg gactgtgaac ggacaactga gggttgggtt ttttaccacc 7261 aaactggttc cttcaggctc agagttaacg tttgactatc agttccagag atatggaaaa 7321 gaagcccaga aatgtttctg cggatcagcc aattgccggg gttacctggg aggagaaaac 7381 agagtcagca tcagagcagc aggagggaaa atgaagaagg aacgatctcg taagaaggat 7441 tcagtggatg gagagctaga agctctgatg gaaaatggtg agggtctctc tgataaaaac 7501 caggtgctca gcttatcccg gctaatggtt agaattgaaa ctttggagca gaaacttacc 7561 tgtctggaac tcatacagaa cacacactca cagtcctgcc tgaagtcctt tctggaacgt 7621 catgggctgt ctttgttgtg gatctggatg gcagagctag gtgacggccg ggaaagtaac 7681 cagaagcttc aggaagagat tataaagact ttggaacact tgcccattcc tactaaaaat 7741 atgttggagg aaagcaaagt acttccaatt attcaacgct ggtctcagac taagactgct 7801 gtccctccgt tgagtgaagg agatgggtat tctagtgaga atacatcgcg tgctcataca 7861 ccactcaaca cacctgatcc ttccaccaag ctgagcacag aagctgacac agacactccc 7921 aagaaactaa tgtttcgcag actgaaaatt ataagtgaaa atagcatgga cagtgcaatc 7981 tctgatgcaa ccagtgagct agaaggcaag gatggcaaag aggatcttga tcaattagaa 8041 aatgtccctg tagaggaaga ggaagaattg cagtcacaac agctactccc acaacagctg 8101 cctgaatgca aagttgatag tgaaaccaac atagaagcta gtaagctacc tacatctgaa 8161 ccagaagctg acgctgaaat agagcccaaa gagagcaacg gcacaaaact agaagaacct 8221 attaatgaag aaacaccatc ccaagatgaa gaggagggtg tgtctgatgt ggagagtgaa 8281 aggagccaag aacagccaga taaaacagtg gatataagtg atttggccac caaactcctg 8341 gacagttgga aagacctaaa ggaggtatat cgaattccaa agaaaagtca aactgaaaag 8401 gaaaacacaa caactgaacg aggaagggat gctgttggct tcagagatca aacacctgcc 8461 ccgaagactc ctaataggtc aagagagaga gacccagaca agcaaactca aaataaagag 8521 aaaaggaaac gaagaagctc cctctcacca ccctcttctg cctatgagcg gggaacaaaa 8581 aggccagatg acagatatga tacaccaact tctaaaaaga aagtacgaat taaagaccgc 8641 aataaacttt ctacagagga acgccggaag ttgtttgagc aagaggtggc tcaacgggag 8701 gctcagaaac aacagcaaca gatgcagaac ctgggaatga catcaccact gccctatgac 8761 tctcttggtt ataatgcccc gcatcatccc tttgctggtt acccaccagg ttatcccatg 8821 caggcctatg tggatcccag caaccctaat gctggaaagg tgctcctgcc cacacccagc 8881 atggacccag tgtgttctcc tgctccttat gatcatgctc agcccttggt gggacattct 8941 acagaacccc tttctgcccc tccaccagta ccagtggtgc cacatgtggc agctcctgtg 9001 gaagtttcca gttcccagta tgtggcccag agtgatggtg tagtacacca agactccagc 9061 gttgctgtct tgccagtgcc ggcccccggc ccagttcagg gacagaatta tagtgtttgg 9121 gattcaaacc aacagtctgt cagtgtacag cagcagtact ctcctgcaca gtctcaagca 9181 accatatatt atcaaggaca gacatgtcca acagtctatg gtgtgacatc accttattca 9241 cagacaactc caccaattgt acagagttat gcccagccaa gtcttcagta tatccagggg 9301 caacagattt tcacagctca tccacaagga gtggtggtac agccagccgc agcagtgact 9361 acaatagttg caccagggca gcctcagccc ttgcagccat ctgaaatggt tgtgacaaat 9421 aatctcttgg atctgccgcc cccctctcct cccaaaccaa aaaccattgt cttacctccc 9481 aactggaaga cagctcgaga tccagaaggg aagatttatt actaccatgt gatcacaagg 9541 cagactcagt gggatcctcc tacttgggaa agcccaggag atgatgccag ccttgagcat 9601 gaagctgaga tggacctggg aactccaaca tatgatgaaa accccatgaa ggcctcgaaa 9661 aagcccaaga cagcagaagc agacacctcc agtgaactag caaagaaaag caaagaagta 9721 ttcagaaaag agatgtccca gttcatcgtc cagtgcctga acccttaccg gaaacctgac 9781 tgcaaagtgg gaagaattac cacaactgaa gactttaaac atctggctcg caagctgact 9841 cacggtgtta tgaataagga gctgaagtac tgtaagaatc ctgaggacct ggagtgcaat 9901 gagaatgtga aacacaaaac caaggagtac attaagaagt acatgcagaa gtttggggct 9961 gtttacaaac ccaaagagga cactgaatta gagGGATCCG ACTACAAAGA CCATGACGGT 10021 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGCG GCCGCGAAGG 10081 ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG 10141 AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC 10201 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT 10261 ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG 10321 CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC GCCGCCCTAC CTGAGGCCGC 10381 CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT GTGGTGCCTC CTGAACTGCG 10441 TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG GCCTTTGTCC GGCGCTCCCT 10501 TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC TGACCCTGCT TGCTCAACTC 10561 TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT CCAAGCTGTG ACCGGCGCCT 10621 ACGCTAGATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCCG 10681 GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA 10741 CCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG 10801 GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC 10861 GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT 10921 GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC 10981 CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG 11041 TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC 11101 CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT 11161 CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG 11221 TGCCTGAGTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 11281 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 11341 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 11401 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 11461 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 11521 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 11581 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT 11641 TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 11701 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 11761 TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC 11821 GCCTGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA 11881 AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAAATAAGAT CTGCTTTTTG 11941 CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 12001 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 12061 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 12121 TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT 12181 GAATATCAGA GAGTGAGAGG AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 12241 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 12301 CCAAACTCAT CAATGTATCT TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC 12361 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 12421 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGA 12481 CTTTTGCAGA GACGGCCCAA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 12541 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 12601 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 12661 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 12721 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 12781 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 12841 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 12901 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 12961 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 13021 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 13081 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 13141 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 13201 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 13261 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 13321 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 13381 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 13441 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 13501 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 13561 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 13621 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 13681 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 13741 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 13801 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 13861 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 13921 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 13981 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 14041 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 14101 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 14161 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 14221 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 14281 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 14341 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 14401 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 14461 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 14521 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 14581 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 14641 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC 14701 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 14761 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 14821 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 14881 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 14941 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG 15001 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG 15061 TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC 15121 GACGGCCAGT GCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.