pLV3-CMV-CYP7B1-3×FLAG-CopGFP-

pLV3-CMV-CYP7B1-3×FLAG-CopGFP-Puro plasmid

PVT96185

pLV3-CMV-CYP7B1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CYP7B1(human)-3×FLAG-CopGFP-Puro
Alias: NM_004820.5;CBAS3; CP7B; SPG5A
Gene lenght: 1518bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CYP7B1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CYP7B1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CYP7B1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg caggagaagt gtccgcggcc 2341 acgggccgct tttcgctgga gcggttgggc ctcccgggcc tggccctcgc cgcggccctg 2401 ctgctcctgg ccctctgctt gcttgtccgg cgcaccagga gacccggtga gcctccattg 2461 ataaaaggtt ggcttcctta tcttggagtg gtcctgaact tacgaaaaga ccccttaagg 2521 ttcatgaaaa cacttcaaaa gcaacatggt gacactttca cagttcttct tggtggaaag 2581 tacataacat ttatcctgga ccccttccag taccagctag tgataaaaaa tcataaacaa 2641 ttaagctttc gagtattttc taataaatta ttagagaaag catttagcat cagtcagttg 2701 caaaaaaatc atgacatgaa tgatgagctt cacctctgct atcaattttt gcaaggcaaa 2761 tctttggaca tactcttgga aagcatgatg cagaatctaa aacaagtttt tgaaccccag 2821 ctgttaaaaa ccacaagttg ggacacggca gaactgtatc cattctgcag ctcaataata 2881 tttgagatca catttacaac tatatatgga aaagttattg tttgtgacaa caacaaattt 2941 attagtgagc taagagatga ttttttaaaa tttgatgaca agtttgcata tttagtatcc 3001 aacataccca ttgagcttct aggaaatgtc aagtctatta gagagaaaat tataaaatgc 3061 ttctcatcag aaaagttagc caagatgcaa ggatggtcag aagtttttca aagcaggcaa 3121 gatgtcctgg agaaatatta tgtgcacgag gaccttgaaa taggagcaca tcatttaggc 3181 tttctctggg cctctgtggc aaacactatt ccaactatgt tctgggcaat gtattatctt 3241 ctgcggcacc cagaagctat ggcagcagtg cgtgacgaaa ttgaccgttt gctgcagtca 3301 acaggtcaaa agaaagggtc tggatttccc atccacctca ccagagaaca attggacagc 3361 ctaatctgcc tagaaagcag catttttgaa gctttacgac tgtcctcata ttcaaccacc 3421 attcgttttg ttgaggagga tttgactctc agttcagaga ccggggacta ctgtgtgcga 3481 aagggagact tggtagccat ctttcctcca gtcctacatg gtgaccctga aatctttgaa 3541 gctccagagg agtttagata tgatcgtttt atagaagatg gtaagaagaa aaccaccttt 3601 ttcaaaagag ggaaaaagct gaagtgttac ctaatgccgt ttggaactgg aaccagcaaa 3661 tgtccaggcc gattttttgc acttatggaa ataaaacaat tgttggttat acttttaact 3721 tattttgatt tagaaataat tgatgataag cccataggac taaactacag ccgcttgttg 3781 tttggtattc agtatccaga ttctgatgtt ttatttagat acaaagtgaa atctggatcc 3841 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 3901 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 3961 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 4021 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 4081 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 4141 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 4201 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 4261 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 4321 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 4381 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 4441 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 4501 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 4561 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 4621 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 4681 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 4741 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 4801 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 4861 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 4921 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 4981 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 5041 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 5101 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 5161 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 5221 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 5281 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 5341 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 5401 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5461 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5521 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5581 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5641 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5701 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5761 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5821 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5881 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 5941 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6001 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 6061 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 6121 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6181 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6241 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 6301 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6361 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6421 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 6481 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6541 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 6601 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6781 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6841 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6961 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 7021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 7081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 7141 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 7201 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7261 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7321 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7381 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7441 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7501 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7561 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7621 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7681 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7741 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7801 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7861 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7921 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7981 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 8041 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 8101 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 8161 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8221 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8281 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8341 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8401 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8461 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8521 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8581 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8641 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8701 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8761 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8821 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8881 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8941 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 9001 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 9061 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 9121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9421 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9481 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9541 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9601 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9661 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9721 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9781 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.