pCMV-GST-TERT-mCMV-EGFP plasmi

pCMV-GST-TERT-mCMV-EGFP plasmid

PVT96154

pCMV-GST-TERT-mCMV-EGFP plasmid Information

Plasmid name: pCMV-GST-TERT(human)-mCMV-EGFP
Alias: NM_198253.3;CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT;PFBMFT1; TCS1; TP2; TRT
Gene lenght: 3399bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GST-TERT-mCMV-EGFP plasmid Description

pCMV-GST-TERT-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-GST-TERT-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCgc caccatgtcc cctatactag gttattggaa 661 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga 721 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg 781 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat 841 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg 901 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat 961 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat 1021 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac 1081 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg 1141 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat 1201 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac 1261 gtttggtggt ggcgaccatc ctccaaaaGG AGGAGGGGGA AGCatgccgc gcgctccccg 1321 ctgccgagcc gtgcgctccc tgctgcgcag ccactaccgc gaggtgctgc cgctggccac 1381 gttcgtgcgg cgcctggggc cccagggctg gcggctggtg cagcgcgggg acccggcggc 1441 tttccgcgcg ctggtggccc agtgcctggt gtgcgtgccc tgggacgcac ggccgccccc 1501 cgccgccccc tccttccgcc aggtgtcctg cctgaaggag ctggtggccc gagtgctgca 1561 gaggctgtgc gagcgcggcg cgaagaacgt gctggccttc ggcttcgcgc tgctggacgg 1621 ggcccgcggg ggcccccccg aggccttcac caccagcgtg cgcagctacc tgcccaacac 1681 ggtgaccgac gcactgcggg ggagcggggc gtgggggctg ctgctgcgcc gcgtgggcga 1741 cgacgtgctg gttcacctgc tggcacgctg cgcgctcttt gtgctggtgg ctcccagctg 1801 cgcctaccag gtgtgcgggc cgccgctgta ccagctcggc gctgccactc aggcccggcc 1861 cccgccacac gctagtggac cccgaaggcg tctgggatgc gaacgggcct ggaaccatag 1921 cgtcagggag gccggggtcc ccctgggcct gccagccccg ggtgcgagga ggcgcggggg 1981 cagtgccagc cgaagtctgc cgttgcccaa gaggcccagg cgtggcgctg cccctgagcc 2041 ggagcggacg cccgttgggc aggggtcctg ggcccacccg ggcaggacgc gtggaccgag 2101 tgaccgtggt ttctgtgtgg tgtcacctgc cagacccgcc gaagaagcca cctctttgga 2161 gggtgcgctc tctggcacgc gccactccca cccatccgtg ggccgccagc accacgcggg 2221 ccccccatcc acatcgcggc caccacgtcc ctgggacacg ccttgtcccc cggtgtacgc 2281 cgagaccaag cacttcctct actcctcagg cgacaaggag cagctgcggc cctccttcct 2341 actcagctct ctgaggccca gcctgactgg cgctcggagg ctcgtggaga ccatctttct 2401 gggttccagg ccctggatgc cagggactcc ccgcaggttg ccccgcctgc cccagcgcta 2461 ctggcaaatg cggcccctgt ttctggagct gcttgggaac cacgcgcagt gcccctacgg 2521 ggtgctcctc aagacgcact gcccgctgcg agctgcggtc accccagcag ccggtgtctg 2581 tgcccgggag aagccccagg gctctgtggc ggcccccgag gaggaggaca cagacccccg 2641 tcgcctggtg cagctgctcc gccagcacag cagcccctgg caggtgtacg gcttcgtgcg 2701 ggcctgcctg cgccggctgg tgcccccagg cctctggggc tccaggcaca acgaacgccg 2761 cttcctcagg aacaccaaga agttcatctc cctggggaag catgccaagc tctcgctgca 2821 ggagctgacg tggaagatga gcgtgcggga ctgcgcttgg ctgcgcagga gcccaggggt 2881 tggctgtgtt ccggccgcag agcaccgtct gcgtgaggag atcctggcca agttcctgca 2941 ctggctgatg agtgtgtacg tcgtcgagct gctcaggtct ttcttttatg tcacggagac 3001 cacgtttcaa aagaacaggc tctttttcta ccggaagagt gtctggagca agttgcaaag 3061 cattggaatc agacagcact tgaagagggt gcagctgcgg gagctgtcgg aagcagaggt 3121 caggcagcat cgggaagcca ggcccgccct gctgacgtcc agactccgct tcatccccaa 3181 gcctgacggg ctgcggccga ttgtgaacat ggactacgtc gtgggagcca gaacgttccg 3241 cagagaaaag agggccgagc gtctcacctc gagggtgaag gcactgttca gcgtgctcaa 3301 ctacgagcgg gcgcggcgcc ccggcctcct gggcgcctct gtgctgggcc tggacgatat 3361 ccacagggcc tggcgcacct tcgtgctgcg tgtgcgggcc caggacccgc cgcctgagct 3421 gtactttgtc aaggtggatg tgacgggcgc gtacgacacc atcccccagg acaggctcac 3481 ggaggtcatc gccagcatca tcaaacccca gaacacgtac tgcgtgcgtc ggtatgccgt 3541 ggtccagaag gccgcccatg ggcacgtccg caaggccttc aagagccacg tctctacctt 3601 gacagacctc cagccgtaca tgcgacagtt cgtggctcac ctgcaggaga ccagcccgct 3661 gagggatgcc gtcgtcatcg agcagagctc ctccctgaat gaggccagca gtggcctctt 3721 cgacgtcttc ctacgcttca tgtgccacca cgccgtgcgc atcaggggca agtcctacgt 3781 ccagtgccag gggatcccgc agggctccat cctctccacg ctgctctgca gcctgtgcta 3841 cggcgacatg gagaacaagc tgtttgcggg gattcggcgg gacgggctgc tcctgcgttt 3901 ggtggatgat ttcttgttgg tgacacctca cctcacccac gcgaaaacct tcctcaggac 3961 cctggtccga ggtgtccctg agtatggctg cgtggtgaac ttgcggaaga cagtggtgaa 4021 cttccctgta gaagacgagg ccctgggtgg cacggctttt gttcagatgc cggcccacgg 4081 cctattcccc tggtgcggcc tgctgctgga tacccggacc ctggaggtgc agagcgacta 4141 ctccagctat gcccggacct ccatcagagc cagtctcacc ttcaaccgcg gcttcaaggc 4201 tgggaggaac atgcgtcgca aactctttgg ggtcttgcgg ctgaagtgtc acagcctgtt 4261 tctggatttg caggtgaaca gcctccagac ggtgtgcacc aacatctaca agatcctcct 4321 gctgcaggcg tacaggtttc acgcatgtgt gctgcagctc ccatttcatc agcaagtttg 4381 gaagaacccc acatttttcc tgcgcgtcat ctctgacacg gcctccctct gctactccat 4441 cctgaaagcc aagaacgcag ggatgtcgct gggggccaag ggcgccgccg gccctctgcc 4501 ctccgaggcc gtgcagtggc tgtgccacca agcattcctg ctcaagctga ctcgacaccg 4561 tgtcacctac gtgccactcc tggggtcact caggacagcc cagacgcagc tgagtcggaa 4621 gctcccgggg acgacgctga ctgccctgga ggccgcagcc aacccggcac tgccctcaga 4681 cttcaagacc atcctggact gaGGATCCGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC 4741 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT 4801 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT 4861 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG 4921 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGTCTAGA GATATACTGA 4981 GTCATTAGGG ACTTTCCAAT GGGTTTTGCC CAGTACATAA GGTCAATAGG GGTGAATCAA 5041 CAGGAAAGTC CCATTGGAGC CAAGTACACT GAGTCAATAG GGACTTTCCA TTGGGTTTTG 5101 CCCAGTACAA AAGGTCAATA GGGGGTGAGT CAATGGGTTT TTCCCATTAT TGGCACGTAC 5161 ATAAGGTCAA TAGGGGTGAG TCATTGGGTT TTTCCAGCCA aTTTAATTAA AACGCCATGT 5221 ACTTTCCCAC CATTGACGTC AATGGGCTAT TGAAACTAAT GCAACGTGAC CTTTAAACGG 5281 TACTTTCCCA TAGCTGATTA ATGGGAAAGT ACCGTTCTCG AGCCAATACA CGTCAATGGG 5341 AAGTGAAAGG GCAGCCAAAA CGTAACACCG CCCCGGTTTT CCCCTGGAAA TTCCATATTG 5401 GCACGCATTC TATTGGCTGA GCTGCGTTCT ACGTGGGTAT AAGAGGCGCG ACCAGCGTCG 5461 GTACCGTCGC AGTCTTCGGT CTGACCACCG TAGAACGCAG ATCaccggtc gccaccatgg 5521 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 5581 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 5641 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 5701 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 5761 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 5821 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 5881 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 5941 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 6001 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 6061 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 6121 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 6181 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg 6241 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 6301 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 6361 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6421 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6481 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 6541 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 6601 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6661 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6721 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6781 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6841 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 6901 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 6961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 7021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg 7081 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 7141 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 7201 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 7261 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 7321 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 7381 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 7441 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 7501 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 7561 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 7621 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 7681 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 7741 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 7801 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 7861 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 7921 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 7981 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 8041 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 8101 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 8161 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 8221 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 8281 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 8341 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 8401 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 8461 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 8521 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 8581 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 8641 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 8701 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 8761 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 8821 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 8881 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 8941 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 9001 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 9061 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 9121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 9181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 9241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 9301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 9361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 9421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 9481 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 9541 ttctgtggat aaccgtatta ccgccatgca t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.