pCMV-GST-MCS-mCMV-EGFP plasmid

pCMV-GST-MCS-mCMV-EGFP plasmid

PVT95933

pCMV-GST-MCS-mCMV-EGFP plasmid Information

Plasmid name: pCMV-GST-MCS-mCMV-EGFP
Alias:
Gene lenght:
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GST-MCS-mCMV-EGFP plasmid Description

pCMV-GST-MCS-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-GST-MCS-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCgc caccatgtcc cctatactag gttattggaa 661 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga 721 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg 781 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat 841 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg 901 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat 961 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat 1021 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac 1081 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg 1141 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat 1201 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac 1261 gtttggtggt ggcgaccatc ctccaaaaGG AGGAGGGGGA AGCGTCGACG CAAGCTTACG 1321 CGTaGGATCC GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA 1381 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 1441 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 1501 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 1561 TGCTGGGGAT GCGGTGGGCT CTATGGTCTA GAGATATACT GAGTCATTAG GGACTTTCCA 1621 ATGGGTTTTG CCCAGTACAT AAGGTCAATA GGGGTGAATC AACAGGAAAG TCCCATTGGA 1681 GCCAAGTACA CTGAGTCAAT AGGGACTTTC CATTGGGTTT TGCCCAGTAC AAAAGGTCAA 1741 TAGGGGGTGA GTCAATGGGT TTTTCCCATT ATTGGCACGT ACATAAGGTC AATAGGGGTG 1801 AGTCATTGGG TTTTTCCAGC CAaTTTAATT AAAACGCCAT GTACTTTCCC ACCATTGACG 1861 TCAATGGGCT ATTGAAACTA ATGCAACGTG ACCTTTAAAC GGTACTTTCC CATAGCTGAT 1921 TAATGGGAAA GTACCGTTCT CGAGCCAATA CACGTCAATG GGAAGTGAAA GGGCAGCCAA 1981 AACGTAACAC CGCCCCGGTT TTCCCCTGGA AATTCCATAT TGGCACGCAT TCTATTGGCT 2041 GAGCTGCGTT CTACGTGGGT ATAAGAGGCG CGACCAGCGT CGGTACCGTC GCAGTCTTCG 2101 GTCTGACCAC CGTAGAACGC AGATCaccgg tcgccaccat ggtgagcaag ggcgaggagc 2161 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 2221 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 2281 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 2341 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 2401 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 2461 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 2521 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 2581 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 2641 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 2701 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 2761 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 2821 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat 2881 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 2941 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 3001 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 3061 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg 3121 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 3181 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 3241 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 3301 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 3361 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 3421 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 3481 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 3541 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 3601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 3661 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg 3721 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3781 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 3841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 3901 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 3961 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 4021 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat 4081 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 4141 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 4201 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 4261 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 4321 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 4381 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 4441 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 4501 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 4561 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 4621 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 4681 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 4741 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 4801 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 4861 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 4921 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 4981 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 5041 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg 5101 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt 5161 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca 5221 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga 5281 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc 5341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 5401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 5461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 5521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 5581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 5641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 5701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 5761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 5821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 5881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 5941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 6001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 6061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 6121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 6181 taccgccatg cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.