FLHKI-pGFPN3 plasmid

FLHKI-pGFPN3 plasmid

PVT96147

FLHKI-pGFPN3 plasmid Information

Plasmid name: FLHKI-pGFPN3
Alias: -
Gene lenght: 2751bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

FLHKI-pGFPN3 plasmid Description

FLHKI-pGFPN3 is a plasmid suitable for protein expression in mammalian cells.

FLHKI-pGFPN3 plasmid Sequence

1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGAT GATCGCCGCG CAGCTCCTGG CCTATTACTT CACGGAGCTG 661 AAGGATGACC AGGTCAAAAA GATTGACAAG TATCTGTATG CCATGCGGCT CTCCGATGAA 721 ACTCTCATAG ATATCATGAC TCGCTTCAGG AAGGAGATGA AGAATGGCCT CTCCCGGGAT 781 TTTAATCCAA CAGCCACAGT CAAGATGTTG CCAACATTCG TAAGGTCCAT TCCTGATGGC 841 TCTGAAAAGG GAGATTTCAT TGCCCTGGAT CTTGGTGGGT CTTCCTTTCG AATTCTGCGG 901 GTGCAAGTGA ATCATGAGAA AAACCAGAAT GTTCACATGG AGTCCGAGGT TTATGACACC 961 CCAGAGAACA TCGTGCACGG CAGTGGAAGC CAGCTTTTTG ATCATGTTGC TGAGTGCCTG 1021 GGAGATTTCA TGGAGAAAAG GAAGATCAAG GACAAGAAGT TACCTGTGGG ATTCACGTTT 1081 TCTTTTCCTT GCCAACAATC CAAAATAGAT GAGGCCATCC TGATCACCTG GACAAAGCGA 1141 TTTAAAGCGA GCGGAGTGGA AGGAGCAGAT GTGGTCAAAC TGCTTAACAA AGCCATCAAA 1201 AAGCGAGGGG ACTATGATGC CAACATCGTA GCTGTGGTGA ATGACACAGT GGGCACCATG 1261 ATGACCTGTG GCTATGACGA CCAGCACTGT GAAGTCGGCC TGATCATCGG CACTGGCACC 1321 AATGCTTGCT ACATGGAGGA ACTGAGGCAC ATTGATCTGG TGGAAGGAGA CGAGGGGAGG 1381 ATGTGTATCA ATACAGAATG GGGAGCCTTT GGAGACGATG GATCATTAGA AGACATCCGG 1441 ACAGAGTTTG ACAGGGAGAT AGACCGGGGA TCCCTCAACC CTGGAAAACA GCTGTTTGAG 1501 AAGATGGTCA GTGGCATGTA CTTGGGAGAG CTGGTTCGAC TGATCCTAGT CAAGATGGCC 1561 AAGGAGGGCC TCTTATTTGA AGGGCGGATC ACCCCGGAGC TGCTCACCCG AGGGAAGTTT 1621 AACACCAGTG ATGTGTCAGC CATCGAAAAG AATAAGGAAG GCCTCCACAA TGCCAAAGAA 1681 ATCCTGACCC GCCTGGGAGT GGAGCCGTCC GATGATGACT GTGTCTCAGT CCAGCACGTT 1741 TGCACCATTG TCTCATTTCG CTCAGCCAAC TTGGTGGCTG CCACACTGGG CGCCATCTTG 1801 AACCGCCTGC GTGATAACAA GGGCACACCC AGGCTGCGGA CCACGGTTGG TGTCGACGGA 1861 TCTCTTTACA AGACGCACCC ACAGTATTCC CGGCGTTTCC ACAAGACTCT AAGGCGCTTG 1921 GTGCCAGACT CCGATGTGCG CTTCCTCCTC TCGGAGAGTG GCAGCGGCAA GGGGGCTGCC 1981 ATGGTGACGG CGGTGGCCTA CCGCTTGGCC GAGCAGCACC GGCAGATAGA GGAGACCCTG 2041 GCTCATTTCC ACCTCACCAA AGACATGCTG CTGGAGGTGA AGAAGAGGAT GCGGGCCGAG 2101 ATGGAGCTGG GGCTGAGGAA GCAGACGCAC AACAATGCCG TGGTTAAGAT GCTGCCCTCC 2161 TTCGTCCGGA GAACTCCCGA CGGGACCGAG AATGGTGACT TCTTGGCCCT GGATCTTGGA 2221 GGAACCAATT TCCGTGTGCT GCTGGTGAAA ATCCGTAGTG GGAAAAAGAG AACGGTGGAA 2281 ATGCACAACA AGATCTACGC CATTCCTATT GAAATCATGC AGGGCACTGG GGAAGAGCTG 2341 TTTGATCACA TTGTCTCCTG CATCTCTGAC TTCTTGGACT ACATGGGGAT CAAAGGCCCC 2401 AGGATGCCTC TGGGCTTCAC GTTCTCATTT CCCTGCCAGC AGACGAGTCT GGACGCGGGA 2461 ATCTTGATCA CGTGGACAAA GGGTTTTAAG GCAACAGACT GCGTGGGCCA CGATGTAGTC 2521 ACCTTACTAA GGGATGCGAT AAAAAGGAGA GAGGAATTTG ACCTGGACGT GGTGGCTGTG 2581 GTCAACGACA CAGTGGGCAC CATGATGACC TGTGCTTATG AGGAGCCCAC CTGTGAGGTT 2641 GGACTCATTG TTGGGACCGG CAGCAATGCC TGCTACATGG AGGAGATGAA GAACGTGGAG 2701 ATGGTGGAGG GGGACCAGGG GCAGATGTGC ATCAACATGG AGTGGGGGGC CTTTGGGGAC 2761 AACGGGTGTC TGGATGATAT CAGGACACAC TACGACAGAC TGGTGGACGA ATATTCCCTA 2821 AATGCTGGGA AACAAAGGTA TGAGAAGATG ATCAGTGGTA TGTACCTGGG TGAAATCGTC 2881 CGCAACATCT TAATCGACTT CACCAAGAAG GGATTCCTCT TCCGAGGGCA GATCTCTGAG 2941 ACGCTGAAGA CCCGGGGCAT CTTTGAGACC AAGTTTCTCT CTCAGATCGA GAGTGACCGA 3001 TTAGCACTGC TCCAGGTCCG GGCTATCCTC CAGCAGCTAG GTCTGAATAG CACCTGCGAT 3061 GACAGTATCC TCGTCAAGAC AGTGTGCGGG GTGGTGTCCA GGAGGGCCGC ACAGCTGTGT 3121 GGCGCAGGCA TGGCTGCGGT TGTGGATAAG ATCCGCGAGA ACAGAGGACT GGACCGTCTG 3181 AATGTGACTG TGGGAGTGGA CGGGACACTC TACAAGCTTC ATCCACACTT CTCCAGAATC 3241 ATGCACCAGA CGGTGAAGGA ACTGTCACCA AAATGTAACG TGTCCTTCCT CCTGTCTGAG 3301 GATGGCAGCG GCAAGGGGGC CGCCCTCATC ACGGCCGTGG GCGTGCGGTT ACGCACAGAG 3361 GCAAGCAGCG GTACCGCGGG CCCGGGATCC ATCGCCACCA TGGTGAGCAA GGGCGAGGAG 3421 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG 3481 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 3541 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC 3601 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 3661 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC 3721 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 3781 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC 3841 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG 3901 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 3961 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC 4021 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 4081 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAA GCGGCCGCGA CTCTAGATCA 4141 TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC 4201 CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT 4261 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 4321 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGGCGTA AATTGTAAGC 4381 GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA 4441 TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT 4501 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG 4561 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT 4621 TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA 4681 GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG 4741 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG 4801 CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 4861 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 4921 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG AGGCGGAAAG AACCAGCTGT 4981 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 5041 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 5101 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC 5161 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 5221 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT 5281 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA 5341 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 5401 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 5461 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 5521 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 5581 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 5641 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 5701 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 5761 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 5821 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC 5881 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 5941 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 6001 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 6061 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 6121 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 6181 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 6241 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 6301 AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG 6361 GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG 6421 TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC 6481 ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG 6541 AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCC TCAGGTTACT 6601 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 6661 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 6721 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 6781 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 6841 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTtC 6901 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 6961 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7021 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7081 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7141 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7201 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7261 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7321 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7381 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7441 TTACCGCCAT GCAT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.