pLV3-CMV-Otud7b-3×HA-CopGFP-Bl

pLV3-CMV-Otud7b-3×HA-CopGFP-Blast plasmid

PVT96128

pLV3-CMV-Otud7b-3×HA-CopGFP-Blast plasmid Information

Plasmid name: pLV3-CMV-Otud7b(mouse)-3×HA-CopGFP-Blast
Alias: NM_001025613.1;2900060B22Rik; 4930463P07Rik; CEZANNE;Trabid2; Za20d1
Gene lenght: 2520bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Otud7b-3×HA-CopGFP-Blast plasmid Description

pLV3-CMV-Otud7b-3×HA-CopGFP-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Otud7b-3×HA-CopGFP-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga ccctggacat ggatgctgtc 2341 ctgtcggatt ttgttcgttc cacaggtgca gagccagggc tggctcgaga tcttctagaa 2401 ggaaagaact gggatgtgag tgctgccctc agtgattttg aacagttgcg tcaagtccat 2461 gctgggaacc tatccccacc ctttagtgga gggagtactt gccctaagac ccctgaaaaa 2521 gggggttcgg atagagagcc cactcgccct tctcgaccca tccttcagag acaagatgac 2581 gtcattcaag aaaaacgcct gtctaggggc atctcccacg ccagctccag cattgtttcc 2641 ctggcccgct cccatgtctc ctccaatggt gggggtgggg ggagcagtga gcaccccctg 2701 gaaatgccca tctgtgcctt ccagcttcca gatctcactg tgtacaaaga agacttccgc 2761 agcttcatag agcgagacct cattgagcag tccatgctgg ttgccttgga acaggcaggg 2821 cgtctgaatt ggtgggttag tatggactcc acctgccaga ggctgctccc tttagcaact 2881 actggggatg ggaactgcct cctgcatgca gcatctcttg ggatgtgggg tttccatgat 2941 agagacttgg tgctgaggaa agcgctgtat gcactgatgg agaagggggt ggagaaggaa 3001 gcactgagaa ggcgctggag gtggcagcaa acacagcaga ataaagagtc aggactggtg 3061 tatacggaag atgagtggca gaaggaatgg aatgaattga tcaaacttgc ttcaagtgaa 3121 cccagaatgc atctgggttc caacggagcc agtggtggtg gagtggagag ctccgaggag 3181 ccggtatatg aaagcctaga ggaattccat gtcttcgtcc ttgctcatgt gctcaagagg 3241 cccatagtgg tcgtggcaga caccatgctg agggactctg gaggggaagc atttgcccct 3301 atcccatttg gagggatcta tctgccattg gaggttccag ccagccagtg tcaccgatcc 3361 cctctggtgc tcgcctatga tcaggcccac ttctctgcgc ttgtgtcgat ggagcagaag 3421 gagagcgcca aagaacaagc tgtgatccca ctcacagatt cagagcacaa gctgctgccc 3481 ctgcactttg ctgtggaccc aggaaagggc tgggagtggg gcaaagatga caatgacaat 3541 gtccgattgg ccagtataat tctgtctctg gaggtgaagt tacatctgct gcatagctat 3601 atgaatgtga agtggatccc actgtcttct gattcacaag ctcccctagc tcagcctgag 3661 tcccccacag cctcagctgg agatgaaccc aggtccactc ctgagtctgg ggagtcagac 3721 aaggagtcgg ttggcagcag ttctctcggc aacgagggca gcaggcggaa ggagaagtca 3781 aagcgagacc gggagaagga caagaagaga gcagattccg tggctaacaa actgggcagc 3841 tttggcaaaa ccttgggcag caagcttaag aagaacatgg ggggcctgat gcacagcaag 3901 ggccccaagc ctggagggct ggggagtggg tctggaataa gcagtggtac ggagacatta 3961 gaaaagaaga agaagaacaa cacattgaag agctggaagg gtggcaagga agaggctgct 4021 ggggatgggc ctgtgtctga gaagcccccg tctgagtctg ttggcaatgg cgggagcaag 4081 tatagccagg aggtgatgca aagcctgagc actatgagga tcgcaatgca aggggagggc 4141 aagtacattt ttgttggaac cctgaagatg ggccaccggc accagtatca ggaggagatg 4201 atccagcgtt accttgcaga tgctgaggaa agattcctgg cagagcagaa gcagaaggag 4261 gttgagagga agatcatgaa tggagggtta gttagtgggc ctcctccagc caaaaagcca 4321 gagccggacg gtggggagga ccagcccagt gactcccccg cagagcccaa ggcgatggcc 4381 ttctctactg cttaccctgg gggctttact atccctcggc cttctggggg tggagtccac 4441 tgccaggaac cccggaggca attggcaggg gggccatgtg tagggggctt accatcatat 4501 gccacctttc ccagacagta tcctgggcga ccgtaccccc accaggacaa catcccagct 4561 ctggagccag gcaaagatgg agttcacagg ggtgccttgt taccacccca gttccgtgtg 4621 gctgattcct atagcaatgg ctacagagag cccccagagc cagatggatg ggctggagct 4681 ccacggggac ttcccccaac ccagaccaaa tgcaaacaac cgaactgcag cttctatgga 4741 caccctgaga caaacaactt gtgctcttgc tgttacaggg aagaactgag gcggagggag 4801 cgggaacctg gtggggaact gctggcacac aggttcCTCG AGTACCCATA CGACGTACCT 4861 GATTATGCAA CTTATCCTTA TGATGTACCT GATTATGCTA GCTATCCTTA TGATGTCCCA 4921 GACTACGCAT AGggatccCT GCGATATCCA GCgcggccgc Gaaggatctg cgatcgctcc 4981 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 5041 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 5101 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 5161 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 5221 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 5281 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 5341 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 5401 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 5461 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 5521 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 5581 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 5641 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 5701 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 5761 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 5821 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 5881 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 5941 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 6001 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 6061 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 6121 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 6181 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 6241 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 6301 tgcggtgacg tggaggagaa tcccggccct ATGGCCAAGC CTTTGTCTCA AGAAGAATCC 6361 ACCCTCATTG AAAGAGCAAC GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC 6421 GTCGCCAGCG CAGCTCTCTC TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT 6481 TTTACTGGGG GACCTTGTGC AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT 6541 GGCAACCTGA CTTGTATCGT CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC 6601 GGACGGTGCC GACAGGTGCT TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC 6661 AGTGATGGAC AGCCGACGGC AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG 6721 GAGGGCTAAG TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 6781 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 6841 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 6901 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 6961 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 7021 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 7081 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC 7141 TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 7201 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 7261 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 7321 CCGCctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 7381 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt 7441 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 7501 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 7561 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 7621 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 7681 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 7741 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 7801 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg 7861 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 7921 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 7981 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat 8041 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 8101 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 8161 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 8221 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 8281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 8341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 8401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 8461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 8521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 8581 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 8641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 8701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 8761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 8821 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 8881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 8941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 9001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 9061 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 9121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 9181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 9241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 9301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 9361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 9421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 9481 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 9541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 9601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 9661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 9721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 9781 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 9841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 9901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 9961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 10021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 10081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 10141 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 10201 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt 10261 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 10321 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 10381 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 10441 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac 10501 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 10561 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 10621 acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.