pLV3-CMV-Gsdmd-3×HA-CopGFP-Bla

pLV3-CMV-Gsdmd-3×HA-CopGFP-Blast plasmid

PVT90321

pLV3-CMV-Gsdmd-3×HA-CopGFP-Blast plasmid Information

Plasmid name: pLV3-CMV-Gsdmd(mouse)-3×HA-CopGFP-Blast
Alias: NM_026960.4;1810036L03Rik; DF5L; Dfna5l; GsdmD-1;Gsdmdc1; M2-4
Gene lenght: 1461bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Gsdmd-3×HA-CopGFP-Blast plasmid Description

pLV3-CMV-Gsdmd-3×HA-CopGFP-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Gsdmd-3×HA-CopGFP-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc catcggcctt tgagaaagtg 2341 gtcaagaatg tgatcaagga ggtaagcggc agcagaggcg atctcattcc ggtggacagc 2401 ctgcggaact ccaccagctt caggccctac tgccttctga acaggaaatt ttcaagctca 2461 aggttctgga aaccccgtta ttcatgtgtc aacctgtcaa tcaaggacat cctggagccc 2521 agtgctccag aaccagaacc ggagtgtttt ggctccttca aagtctctga tgtcgtcgat 2581 gggaacattc agggcagagt gatgttgtca ggcatgggag aagggaaaat ttctggtggg 2641 gctgcagtgt ctgacagttc cagtgcctcc atgaatgtgt gtatactgcg tgtgactcag 2701 aagacctggg agaccatgca gcatgaaagg caccttcagc agcctgagaa caaaatcctg 2761 caacagcttc ggagtcgtgg ggatgacctg tttgtggtga ccgaggtgct gcagacaaag 2821 gaggaagtgc agatcactga ggtccacagc caagagggct caggccagtt tacgctgcct 2881 ggagctttat gcttgaaggg tgaaggcaag ggccaccaaa gccggaagaa gatggtgacc 2941 attcctgcag gcagcatcct ggcattccga gtggcccaac tgcttattgg ctctaaatgg 3001 gatatccttc tcgtctcaga tgagaaacag aggacctttg agccctcctc aggtgacaga 3061 aaagcagtgg gccagaggca ccatggcctc aatgtgcttg ctgcgctttg ttccatcgga 3121 aagcagctca gtctcctgtc agatgggatt gatgaggagg aattaattga ggcggcagac 3181 ttccagggcc tgtatgctga ggtgaaggct tgctcctcag aactggagag cttggaaatg 3241 gagttgagac aacagatact ggtgaacatc ggaaagattt tacaggacca gcccagcatg 3301 gaagccttag aggcctcact agggcagggc ctgtgcagtg gcggccaggt ggagcctctg 3361 gacggcccag ctggctgcat ccttgagtgt ctggtgcttg actctggaga actggtgccg 3421 gaactcgcag cccctatctt ctacctgctg ggagcactgg ctgtgctgag tgaaacccag 3481 cagcagctgc tagctaaggc tctggagaca acggtgctgt caaagcagct ggagttggtg 3541 aagcacgtct tggaacagag caccccgtgg caggagcaga gttctgtgtc cctgcccacc 3601 gtgctccttg gggactgctg ggatgaaaag aatcccacct gggtcttgct agaagaatgt 3661 ggcctaaggc tgcaggtaga atccccccag gtgcactggg aaccaacgtc tctgatcccc 3721 acaagtgcgc tctatgcctc cctgttccta ttgtcaagtc taggccagaa accttgtCTC 3781 GAGTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT ATGATGTACC TGATTATGCT 3841 AGCTATCCTT ATGATGTCCC AGACTACGCA TAGggatccC TGCGATATCC AGCgcggccg 3901 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 3961 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4021 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 4081 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4141 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4201 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4261 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 4321 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 4381 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 4441 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 4501 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 4561 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 4621 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 4681 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 4741 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 4801 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 4861 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 4921 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 4981 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 5041 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 5101 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 5161 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 5221 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tATGGCCAAG 5281 CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC 5341 CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG CCGCATCTTC 5401 ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC 5461 ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG TCGCGATCGG AAATGAGAAC 5521 AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG 5581 ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG 5641 CTGCCCTCTG GTTATGTGTG GGAGGGCTAA GTCGACAATC AACCTCTGGA TTACAAAATT 5701 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 5761 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 5821 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 5881 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 5941 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 6001 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 6061 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 6121 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 6181 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 6241 ATCTCCCTTT GGGCCGCCTC CCCGCctggt acctttaaga ccaatgactt acaaggcagc 6301 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6361 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6421 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6481 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6541 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6601 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6661 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 6721 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 6781 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6841 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6901 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca 6961 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 7021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 7081 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 7141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 7201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 8041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 8101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 8161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 8221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 9001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 9061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 9121 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 9181 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 9241 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 9301 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 9361 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 9421 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 9481 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 9541 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.