pLV3-CMV-Kdm3a-3×FLAG-Puro pla

pLV3-CMV-Kdm3a-3×FLAG-Puro plasmid

PVT96121

pLV3-CMV-Kdm3a-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-Kdm3a(mouse)-3×FLAG-Puro
Alias: NM_173001.3;1700105C21Rik; C230043E16Rik; JHDM2a;Jmjd1; Jmjd1a; KDM2A; TGSA; Tsga
Gene lenght: 3969bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-Kdm3a-3×FLAG-Puro plasmid Description

pLV3-CMV-Kdm3a-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Kdm3a-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggtgctc acgctcggag aaagttggcc agtattggtg 2341 gggaagcgat tcctcagtct gtccgcagcc gaaggcaacg aaggcggcca ggacaactgg 2401 gacttggagc gcgttgccga gtggccctgg ctgtcgggga ccattcgagc tgtttcccac 2461 accgacgtta ctaagaaaga cttgaaggtg tgtgtggagt ttgatgggga gtcttggaga 2521 aagagaagat ggatagatgt ctacagcctt cagagaaaag catttttagt agagcataac 2581 ctggttttgg cagaacgaaa atcacctgaa gttcctgagc aagttattca gtggcctgca 2641 ataatgtaca aatctcttct agacaaagct ggcttgggag ccataacttc tgttcggttt 2701 cttggagatc aacaaagtgt atttgtttcc aaagaccttt tgaaacctat acaggatgtt 2761 aacagtcttc ggctttccct tactgataat cagacagtca gtaaggaatt tcaagctttg 2821 attgtaaaac atttggatga aagccatctt ttacaaggtg acaagaacct tgttggttca 2881 gaagtaaaaa tttatagctt ggacccatct actcagtggt tttcagcaac tgttgtacat 2941 ggaaacccat catccaaaac tcttcaagtc aactgtgagg agattccagc actgaaaatt 3001 gtcgacccag cactgattca tgttgaagtt gtacatgaca actttgtgac atgtggtaat 3061 tctacaagaa ctggagctgt aaaacgcaag tcttctgaga ataacggaag ttcggtttct 3121 aaacaagcaa aatcttgttc tgaggcctct cccagtatgt gtcctgtaca gtctgttccc 3181 acaacagtgt ttaaggagat cctgcttggc tgtactgcag caactccatc tagcaaggac 3241 ccaagacagc aaaatactcc ccaggcagcc aattctccac ctaacattgg agcaaaactt 3301 cctcaaggat gtcataagca gaacttacca gaagaacttt cttcctgtct aaacacaaaa 3361 cctgaagtac cgagaacaaa accagatgtc tgcaaagaag gattactttc ttcaaaatct 3421 tctcaggttg gagctggaga cttgaaaatt ctgagtgagc ccaaaggtag ctgtatccag 3481 cctaaaacaa acactgatca ggagagcaga ctggagtctg ctccacagcc agtcactggc 3541 cttccaaagg agtgcttgcc tgcaaagact tcctctaagg cagaactgga cattgccacc 3601 actcctgaac tgcagaagca tctagaacat gcagcttcca catccgatga cctttcagat 3661 aagccagaag tgaaagcagg tgtcactagc cttaatagtt gtgcagaaaa gaaggtcgaa 3721 ccttcacatt taggttccca gtcacagaat ttaaaggaaa cttcagtaaa agtagataat 3781 gaaagctgtt gtacaagaag cagtaataaa acccagactc ccccagcccg gaagtcagtt 3841 ttgacagacc cagataaagt caggaagctg cagcagagcg gagaggcctt tgttcaggat 3901 gactcctgtg ttaacatcgt ggcacagctg cccaagtgtc gggagtgtcg actagacagc 3961 ctgcgcaagg ataaggacca gcagaaggac tctcctgtgt tttgtcgctt tttccacttc 4021 aggagattac aattcaacaa gcatggtgtg ttgcgggtag aaggcttctt aacaccaaac 4081 aagtatgaca gtgaagcgat tggcttgtgg ctgcctttga ccaaaaatgt tgtggggact 4141 gatttggaca cagcaaaata tatcctggcc aatattggag accacttctg tcaaatggtg 4201 atttctgaga aggaagctat gtcgactatt gagccacaca gacaggttgc ttggaaacga 4261 gctgtcaaag gagttagaga aatgtgtgat gtgtgtgaca caaccatttt caacctgcac 4321 tgggtgtgcc ctcggtgtgg gtttggagta tgtgtagatt gctaccggat gaagaggaag 4381 aactgccaac agggtgctgc ctacaagact ttctcttgga taaggtgtgt gaagagtcag 4441 atacatgagc ctgagaacct gatgcccaca cagattattc ctggcaaagc cctctacgat 4501 gttggagaca ttgtgcattc tgtcagagca aaatggggca taaaggccaa ttgtccctgc 4561 tccaacaggc agttcaagct cttctcaaag ccagccttaa aggaagacct gaaacagaca 4621 tccttgtctg gagaaaaacc aactcttggg accatggtcc agcaaagttc ccctgttttg 4681 gagccagtgg ctgtgtgcgg ggaagcagcc tccaagccag ccagcagcgt gaagcccacc 4741 tgtcccacca gcacttcacc tttaaactgg ctagctgacc ttaccagtgg gaatgtcaac 4801 aaggagaata aggaaaaaca gctgactatg ccaattttaa agaatgaaat caaatgcctt 4861 ccacccttgc cccctctgaa caagcccagc acagtcctcc atacttttaa cagcaccatc 4921 ttgacacctg tgagcaacaa taattcaggt ttccttagaa atcttttgaa ttcatccaca 4981 gcaaagacag aaaatggatt gaaaaacaca cccaaaattc ttgatgacat ctttgcctct 5041 ttggtgcaaa acaagacttc ttctgattca tccaagaggc ctcaaggact gacaatcaag 5101 cctagcattc ttggctttga cactcctcac tactggctgt gtgacaaccg cctgctgtgc 5161 ttgcaagacc ccaacaataa gagcaattgg aatgttttta gggaatgctg gaaacaaggg 5221 cagccagtga tggtgtcggg cgtgcatcat aaattaaaca ctgaactctg gaaacccgag 5281 tccttcagaa aagagtttgg tgagcaggaa gtagacctag tcaattgtag gaccaatgaa 5341 atcatcactg gagccacagt cggagacttc tgggatggat ttgaagatgt tccaaaccgt 5401 ttgaaaaacg acaaagaaaa agaaccaatg gtgttgaaac ttaaggactg gccgccagga 5461 gaagacttca gagacatgat gccttccagg tttgatgatc tgatggccaa cattccactg 5521 cctgagtaca ccaggcgaga tggcaaactg aacctggctt ccagactgcc aaactacttt 5581 gtacggccag acctgggccc caagatgtac aatgcttatg gattgatcac tccagaggat 5641 cggaaatatg ggaccacaaa tcttcactta gatgtatctg atgcagccaa tgtcatggtt 5701 tatgtgggaa ttcccaaagg acagtgtgaa caagaagaag aagtccttag aaccatccaa 5761 gatggagatt ctgatgaact cacaatcaag agatttattg aaggaaaaga gaagccagga 5821 gccctttggc acatatatgc tgctaaagac acagagaaga taagagaatt ccttaaaaag 5881 gtatcagagg agcagggtca agacaaccct gcagaccatg accctatcca cgatcagagc 5941 tggtatttag accgatcgct gagaaagcgc ctctatcaag agtacggcgt gcaaggctgg 6001 gctattgtac agtttcttgg ggatgtggtg tttatcccag caggagcgcc acatcaggtt 6061 cataacttat acagctgtat caaagtggct gaagactttg tgtctccaga gcatgttaaa 6121 cactgcttct ggcttactca ggaattccgt tacttgtcac agactcatac caaccatgaa 6181 gataaattgc aggtgaaaaa tgttatctac catgcagtga aagatgcagt tgctatgctg 6241 aaagccagtg aatccagttt gggcaaacct GGATCCGACT ACAAAGACCA TGACGGTGAT 6301 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGCGGCC GCGAAGGATC 6361 TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG 6421 TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG 6481 GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA 6541 AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG 6601 AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT 6661 CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC 6721 GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG 6781 AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC 6841 GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG 6901 CTAGATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC 6961 CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC 7021 GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT 7081 CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC 7141 GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG 7201 CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA 7261 GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT 7321 GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT 7381 CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC 7441 CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC 7501 CTGAGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 7561 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT 7621 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA 7681 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC 7741 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT 7801 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG 7861 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC 7921 TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC 7981 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT 8041 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC 8101 TGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG 8161 AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAA ATAAGATCTG CTTTTTGCTT 8221 GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA 8281 ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC 8341 TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT 8401 CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA 8461 TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 8521 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 8581 AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAG 8641 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC 8701 CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGACTT 8761 TTGCAGAGAC GGCCCAAATT CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 8821 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 8881 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 8941 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 9001 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 9061 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 9121 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 9181 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 9241 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 9301 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 9361 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 9421 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 9481 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 9541 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 9601 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 9661 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 9721 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 9781 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 9841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 9901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 9961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 10021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 10081 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 10141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 10201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 10261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 10321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 10381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 10441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 10501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 10561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 10621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 10681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 10741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 10801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 10861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 10921 ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA 10981 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA 11041 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG 11101 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA 11161 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG 11221 ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG 11281 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC 11341 TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC 11401 GGCCAGTGCC AAGCTG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.