pLV3-CMV-Mettl3-3×Myc-Fluc-Pur

pLV3-CMV-Mettl3-3×Myc-Fluc-Puro plasmid

PVT92803

pLV3-CMV-Mettl3-3×Myc-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-Mettl3(mouse)-3×Myc-Fluc-Puro
Alias: NM_019721.2;2310024F18Rik; M6A; Spo8
Gene lenght: 1740bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Mettl3-3×Myc-Fluc-Puro plasmid Description

pLV3-CMV-Mettl3-3×Myc-Fluc-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Mettl3-3×Myc-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcGCCAC Catgtcggac acgtggagct ctatccaggc ccataagaaa 2341 cagctggact cgcttcgcga gagattgcag cggcgacgga agcaggactc tgggcacttg 2401 gatttaagga acccagaagc ggcactgtcc ccaaccttcc gtagtgatag tcccgtgcct 2461 actgccccta cctctagcgg ccctaagccc agcacaacat ctgtggcccc tgaactagct 2521 acagaccctg agttagagaa gaagttgcta caccacctct cagatctggc cttgaccttg 2581 cccactgatg ctgtttccat ccgtcttgcc atctctacgc cagatgcacc tgccactcaa 2641 gatggggtag aaagccttct ccagaaattt gccgcccagg agttgattga ggtaaagcga 2701 ggtctcctcc aagatgatgc acatcctact cttgtaactt acgctgacca ctccaagctg 2761 tctgccatga tgggggctgt ggcagaaaag aaaggtcttg gagaggtagc agggaccatc 2821 gcagggcaga aacggcgtgc agaacaggat ttgactacag tgaccacctt tgccagctct 2881 ttagcatctg gtctggcctc ttcagcatca gaaccagcta aggagccggc taagaagtca 2941 aggaagcacg ctgcctccga tgttgatctg gagatagaaa gccttttgaa ccaacagtca 3001 acgaaagaac agcagagcaa gaaggtcagt caggagatcc tagagctatt aaataccaca 3061 acagccaagg aacagtccat tgttgaaaag tttcgctctc gaggtcgggc ccaggtgcaa 3121 gaattttgtg attatgggac caaggaagag tgcatgaaag ccagtgacgc tgaccggcct 3181 tgtcgcaagc tgcacttcag acgaattatc aataagcaca ctgatgaatc tttaggtgac 3241 tgctctttcc ttaacacatg tttccacatg gacacctgca aatatgttca ctatgaaatt 3301 gatgcttgtg ttgattctga gagtcctggc agcaaggagc atatgccaag ccaggagctt 3361 gctcttacac agagtgttgg gggtgactcc agtgctgatc gactctttcc acctcagtgg 3421 atctgttgtg atatccgcta cctggacgtc agtatcttgg gcaaatttgc agttgtgatg 3481 gctgacccac cttgggatat tcacatggag ctaccgtatg ggacattaac agatgatgag 3541 atgcgcaggc tcaatatacc agtgctacag gatgacggct ttcttttcct ctgggtcaca 3601 ggaagggcca tggaattggg cagagaatgt ctgaacctct ggggttatga acgggtggat 3661 gaaatcatct gggtgaagac taatcagctg cagcgcatca ttaggacggg ccggacgggt 3721 cactggttaa accacgggaa ggaacactgc ttggttggtg ttaagggaaa tcctcaagga 3781 ttcaaccagg gcctggactg cgatgtgatt gtagctgagg ttcgttccac cagtcataaa 3841 ccagatgaaa tatatggcat gattgagaga ctgtcccctg gcacccgcaa gattgagtta 3901 tttggacgac cacacaatgt gcagcccaac tggattactc ttggaaacca actggatggg 3961 atacacctac tagacccaga tgtggttgcc aggtttaagc aaaggtatcc ggacggcatc 4021 atctctaaac ctaagaattt aGAACAAAAA CTAATATCAG AGGAAGATTT GGAACAGAAA 4081 CTAATCAGTG AAGAAGATTT AGAGCAAAAG TTAATTTCCG AGGAGGACTT ATAGgcggcc 4141 gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 4201 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4261 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 4321 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4381 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4441 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4501 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 4561 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 4621 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 4681 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgatgga agacgccaaa 4741 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa 4801 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca 4861 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa 4921 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct 4981 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg 5041 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg 5101 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc 5161 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac 5221 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc 5281 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct 5341 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct 5401 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac 5461 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg 5521 tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg 5581 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat 5641 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa 5701 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact 5761 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt 5821 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat 5881 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg 5941 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac 6001 tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa 6061 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc 6121 gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt 6181 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa 6241 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt 6301 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc 6361 ggaaagatcg ccgtgagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 6421 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 6481 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 6541 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 6601 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 6661 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 6721 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 6781 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 6841 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 6901 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 6961 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 7021 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 7081 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 7141 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 7201 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 7261 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 7321 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 7381 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 7441 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 7501 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 7561 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 7621 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct 7681 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 7741 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 7801 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7861 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7921 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7981 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 8041 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 8101 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 8161 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 8221 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 8281 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 8341 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 8401 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 8461 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 8521 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 8581 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 8641 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 8701 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 8761 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 8821 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 8881 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 8941 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 9001 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 9061 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 9121 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 9181 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 9241 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 9301 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 9361 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 9421 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 9481 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 9541 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 9601 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 9661 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 9721 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9781 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 9841 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9901 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9961 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 10021 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 10081 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 10141 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 10201 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 10261 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 10321 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 10381 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 10441 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 10501 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 10561 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 10621 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 10681 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 10741 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 10801 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 10861 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 10921 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 10981 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.