pLV3-U6-Negative-Control-shRNA

pLV3-U6-Negative-Control-shRNA-CopGFP-Hyg plasmid

PVT96086

pLV3-U6-Negative-Control-shRNA-CopGFP-Hyg plasmid Information

Plasmid name: pLV3-U6-Negative-Control-shRNA-CopGFP-Hyg
Alias: TTCTCCGAACGTGTCACGT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence,control
Fragment type: shRNA
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-Negative-Control-shRNA-CopGFP-Hyg plasmid Description

pLV3-U6-Negative-Control-shRNA-CopGFP-Hyg is a plasmid suitable for interference silencing in mammalian cells and can also be used for lentivirus packaging.

pLV3-U6-Negative-Control-shRNA-CopGFP-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgTTCTC CGAACGTGTC ACGTttcaag agaACGTGAC 2221 ACGTTCGGAG AATTTTTTgg atccgcggcc gcGaaggatc tgcgatcgct ccggtgcccg 2281 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa 2341 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg 2401 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa 2461 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat 2521 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt 2581 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa 2641 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg 2701 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt 2761 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag 2821 cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg 2881 cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa 2941 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat 3001 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca 3061 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt 3121 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt 3181 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa 3241 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg 3301 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt 3361 caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg 3421 cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa 3481 gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac 3541 cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga 3601 cgtggaggag aatcccggcc ctatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa 3661 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga 3721 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg 3781 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc 3841 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg 3901 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca 3961 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt 4021 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc 4081 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc 4141 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca 4201 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc 4261 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt 4321 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca 4381 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca 4441 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg 4501 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag 4561 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc 4621 cagcactcgt ccgagggcaa aggaataggt cgacaatcaa cctctggatt acaaaatttg 4681 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4741 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4801 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4861 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 4921 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 4981 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5041 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 5101 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 5161 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 5221 ctccctttgg gccgcctccc cgcggtacct ttaagaccaa tgacttacaa ggcagctgta 5281 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 5341 aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 5401 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 5461 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 5521 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 5581 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 5641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 5701 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 5761 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 5821 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 5881 gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc 5941 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6001 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6061 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6121 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6181 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6241 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6301 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6361 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6421 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6481 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6541 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6601 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6661 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6721 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 6781 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6841 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6901 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6961 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7021 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7081 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7141 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7201 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7261 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7321 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7381 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7441 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7501 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7561 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7621 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7681 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 7741 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7801 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7861 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7921 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 7981 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8041 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 8101 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc 8161 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 8221 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 8281 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 8341 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc 8401 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 8461 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 8521 agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.