pCMV-TEAD2-Neo plasmid

pCMV-TEAD2-Neo plasmid

PVT96077

pCMV-TEAD2-Neo plasmid Information

Plasmid name: pCMV-TEAD2(human)-Neo
Alias: NM_001256658.2;ETF; TEAD-2; TEF-4; TEF4
Gene lenght: 1350bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TEAD2-Neo plasmid Description

pCMV-TEAD2-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TEAD2-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgga gccaggttct 721 aagtcagtgt ctaggtcaga ctggcaacct gaaccacacc agaggcctat aaccccgcta 781 gagcctgggc cagaaaagac acccatagcc cagccagaat cgaagactct gcagggatcc 841 aatacccaac agaagcctgc ttcaaaccaa agacccctca cccagcagga gacccctgca 901 caacatgatg ctgaatccca gaaggaacct agagcccaac aaaaatctgc ttcacaagag 961 gaatttcttg ccccacagaa gcccgcacca cagcaatcac cttacatcca aagggtgctg 1021 ctcactcaac aggaagctgc ctcccagcag ggacctgggc taggaaaaga atctataact 1081 caacaggagc cagcattgag acaaagacat gtagcccagc cagggcctgg gccaggagag 1141 ccacctccag ctcaacaaga agctgaatca acacctgcgg cccaggctaa acctggagcc 1201 aaaagggagc catctgcccc gactgaatct acgtcccaag agacacctga acagtcagac 1261 aagcaaacaa cgccagtcca gggagccaaa tccaagcagg gatctttgac agagctggga 1321 tttctaacaa aacttcagga actatccata cagcgatcag ccctagagtg gaaggcactt 1381 tctgagtggg tcacagattc tgagtcagaa tcagatgtgg gatcatcttc agacacagat 1441 tctccagcca cgatgggtgg aatggtggcc cagggagtga agctaggctt caaaggaaaa 1501 tctggttata aagtgatgtc aggatacagt gggacgtcgc cacatgagaa aaccagtgct 1561 cggaatcaca gacactacca ggatacagcc tcaaggctca tccacaacat ggacctgcgc 1621 acaatgacac agtcgctggt gactctggcg gaggacaaca tagccttctt ctcgagccag 1681 ggtcctgggg aaacggccca gcggctgtca ggcgtttttg ccggtgtacg ggagcaggcg 1741 ctggggctgg agccggccct gggccgcctg ctgggtgtgg cgcacctctt tgacctggac 1801 ccagagacac cggccaacgg gtaccgcagc ctagtgcaca cagcccgctg ctgcctggcg 1861 cacctcctgc acaaatcccg ctatgtggcc tccaaccgcc gcagcatctt cttccgcacc 1921 agccacaacc tggccgagct ggaggcctac ctggctgccc tcacccagct ccgcgctctg 1981 gtctactacg cccagcgcct gctggttacc aatcggccgg gggtactctt ctttgagggc 2041 gacgaggggc tcaccgccga cttcctccgg gagtatgtca cgctgcataa gggatgcttc 2101 tatggccgct gcctgggctt ccagttcacg cctgccatcc ggccattcct gcagaccatc 2161 tccattgggc tggtgtcctt cggggagcac tacaaacgca acgagacagg cctcagtgtg 2221 gccgccagct ctctcttcac cagcggccgc tttgccatcg accccgagct gcgtggggct 2281 gagtttgagc ggatcacaca gaacctggac gtgcacttct ggaaagcctt ctggaacatc 2341 accgagatgg aagtgctatc gtctctggcc aacatggcat cggccaccgt gagggtaagc 2401 cgcctgctca gcctgccacc cgaagccttt gagatgccac tgactgccga ccccacgctc 2461 acggtcacca tctcaccccc actggcccac acaggccctg ggcccgtcct cgtcaggctc 2521 atctcctatg acctgcgtga aggacaggac agtgaggagc tcagcagcct gataaagtcc 2581 aacggccaac ggagcctgga gctgtggccg cgcccccagc aggcaccccg ctcgcggtcc 2641 ctgatagtgc acttccacgg cggtggcttt gtggcccaga cctccagatc ccacgagccc 2701 tacctcaaga gctgggccca ggagctgggc gcccccatca tctccatcga ctactccctg 2761 gcccctgagg cccccttccc ccgtgcgctg gaggagtgct tcttcgccta ctgctgggcc 2821 atcaagcact gcgccctcct tggctcaaca ggggaacgaa tctgccttgc gggggacagt 2881 gcaggcggga acctctgctt caccgtggct cttcgggcag cagcctacgg ggtgcgggtg 2941 ccagatggca tcatggcagc ctacccggcc acaatgctgc agcctgccgc ctctccctcc 3001 cgcctgctga gcctcatgga ccccttgctg cccctcagtg tgctctccaa gtgtgtcagc 3061 gcctatgctg gtgcaaagac ggaggaccac tccaactcag accagaaagc cctcggcatg 3121 atggggctgg tgcggcggga cacagccctg ctcctccgag acttccgcct gggtgcctcc 3181 tcatggctca actccttcct ggagttaagt gggcgcaagt cccagaagat gtcggagccc 3241 atagcagagc cgatgcgccg cagtgtgtct gaagcagcac tggcccagcc ccagggccca 3301 ctgggcacgg attccctcaa gaacctgacc ctgagggact tgagcctgag gggaaactcc 3361 gagacgtcgt cggacacccc cgagatgtcg ctgtcagctg agacacttag cccctccaca 3421 ccctccgatg tcaacttctt attaccacct gaggatgcag gggaagaggc tgaggccaaa 3481 aatgagctga gccccatgga cagaggcctg ggcgtccgtg ccgccttccc cgagggtttc 3541 cacccccgac gctccagcca gggtgccaca cagatgcccc tctactcctc acccatagtc 3601 aagaacccct tcatgtcgcc gctgctggca cccgacagca tgctcaagag cctgccacct 3661 gtgcacatcg tggcgtgcgc gctggacccc atgctggacg actcggtcat gctcgcgcgg 3721 cgactgcgca acctgggcca gccggtgacg ctgcgcgtgg tggaggacct gccgcacggc 3781 ttcctgaccc tagcggcgct gtgccgcgag acgcgccagg ccgcagagct gtgcgtggag 3841 cgcatccgcc tcgtcctcac tcctcccgcc ggagccgggc cgagcgggga gacgggggct 3901 gcgggggtag acgggggctg cggggggcga cacCTCGAGT ACCCATACGA CGTACCTGAT 3961 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 4021 TACGCATAGT GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA 4081 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 4141 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 4201 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 4261 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 4321 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 4381 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 4441 GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 4501 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 4561 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC 4621 TTTGGGCCGC CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT 4681 ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG 4741 GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC 4801 CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA 4861 GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG 4921 ATCTGCGCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG 4981 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 5041 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 5101 TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 5161 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 5221 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 5281 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT 5341 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG 5401 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 5461 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 5521 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 5581 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 5641 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 5701 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 5761 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 5821 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 5881 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 5941 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 6001 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 6061 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 6121 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 6181 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 6241 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 6301 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 6361 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 6421 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 6481 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 6541 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG 6601 TTCGCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 6661 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 6721 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 6781 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 6841 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 6901 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 6961 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 7021 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 7081 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 7141 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 7201 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 7261 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 7321 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 7381 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 7441 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 7501 TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT 7561 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 7621 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 7681 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 7741 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 7801 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 7861 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 7921 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 7981 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 8041 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 8101 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 8161 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 8221 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 8281 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 8341 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 8401 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 8461 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 8521 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 8581 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 8641 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 8701 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 8761 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 8821 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 8881 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 8941 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 9001 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 9061 CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG 9121 GTCGACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC 9181 TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG 9241 CTTGACCGAC AATTGCATGA AGAATCTGCT TAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.