pCMV-RPTOR-6×His-Neo plasmid

pCMV-RPTOR-6×His-Neo plasmid

PVT95777

pCMV-RPTOR-6×His-Neo plasmid Information

Plasmid name: pCMV-RPTOR(human)-6×His-Neo
Alias: NM_020761.3;KOG1; Mip1
Gene lenght: 4005bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-RPTOR-6×His-Neo plasmid Description

pCMV-RPTOR-6×His-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-RPTOR-6×His-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCgccacc atggagtccg aaatgctgca 961 atcgcctctt ctgggcctgg gggaggaaga tgaggctgat cttacagact ggaacctacc 1021 tttggctttt atgaaaaaga ggcactgtga gaaaattgaa ggctccaaat ccttagctca 1081 gagctggagg atgaaggatc ggatgaagac agtcagtgtt gccttagttt tgtgcctgaa 1141 tgttggtgtg gaccctcccg atgtggtgaa gaccacgccc tgtgcacgct tggaatgctg 1201 gatcgatcct ctgtcgatgg gtcctcagaa agctctggaa accatcggtg caaatttaca 1261 gaagcagtac gagaactggc agccaagggc ccggtacaag cagagccttg acccaactgt 1321 ggatgaagtc aagaagctct gcacgtcctt acgtcgcaac gccaaggagg agcgagtcct 1381 ctttcactac aatggccacg gggtgccccg gcccacagtc aacggggagg tctgggtctt 1441 caacaagaac tacacgcagt acatccctct gtccatatat gacctgcaga cgtggatggg 1501 cagcccgtcg atcttcgtct acgactgctc caatgctggc ttgatcgtca agtccttcaa 1561 gcagttcgca ctacagcggg agcaggagct ggaggtagct gcaatcaacc caaatcaccc 1621 tcttgctcag atgcctttgc ctccgtcgat gaaaaactgc atccagctgg cagcctgcga 1681 ggccaccgag ctgctgccca tgatccccga cctcccggct gacctattca cctcctgcct 1741 caccaccccc atcaagatcg ccctgcgctg gttttgcatg cagaaatgtg tcagtctggt 1801 gcctggcgtc acactggatt tgatagaaaa gatccctggc cgcctgaacg acaggaggac 1861 gcccctgggt gaactgaact ggatcttcac agccatcaca gacaccatcg cgtggaacgt 1921 gctcccccgg gatctcttcc aaaagctctt cagacaggac ttgctggtgg ctagtctgtt 1981 tcgaaatttt ttattggcgg aaaggattat gaggtcgtat aactgcactc ccgtcagcag 2041 cccgcgtctg ccgcccacgt acatgcacgc catgtggcaa gcctgggacc tggctgttga 2101 catctgtctg tctcagctgc cgacgatcat cgaggaaggc actgcgtttc ggcacagccc 2161 gttcttcgcc gagcagctga ccgcattcca ggtgtggctc accatgggcg tggagaaccg 2221 aaacccaccc gaacagctgc ccatcgtcct gcaggtgctg ttaagccaag tgcaccggct 2281 gagagcattg gacttgcttg gaagattttt ggacctgggt ccctgggcag tgagcctggc 2341 cttgtctgtc ggcatcttcc cctacgtgct gaagctgctc cagagctcgg cccgagagct 2401 gcggccactt ctcgttttca tctgggccaa gatcctcgca gtggacagct cgtgccaagc 2461 ggacctcgtg aaggacaacg gccacaagta cttcctgtcg gtcctggcgg acccctacat 2521 gccagctgaa caccggacca tgacggcttt cattctcgcc gtgatcgtca acagctatca 2581 cacggggcag gaagcctgcc ttcagggaaa cctcattgcc atctgcctgg agcagctcaa 2641 cgacccgcac cccttgctgc gccagtgggt ggccatctgc ctcggcagga tctggcagaa 2701 cttcgactcg gcgaggtggt gcggcgtgag ggacagcgct catgagaagc tctacagcct 2761 cctctccgac cccattcccg aggtccgctg cgcagcggtc ttcgcccttg gcacgttcgt 2821 gggcaactct gcagagagga cggaccactc caccaccatc gaccacaacg tggccatgat 2881 gctggcccag ctggtcagcg acgggagccc catggtccgg aaggagctgg tggtggctct 2941 gagtcatctt gtggttcagt atgaaagcaa tttctgcacc gtggccctgc agttcataga 3001 agaggaaaag aactacgcct tgccttctcc agcaaccaca gagggaggga gtttgacccc 3061 agtgcgagac agcccgtgca cccccagact tcgttctgtg agctcctatg gaaacatccg 3121 tgctgtcgcc acagccagga gcctcaacaa atctttgcag aacctgagtt tgacagagga 3181 atctggtggc gcggtggcgt tctcccccgg aaacctcagc accagcagca gcgccagcag 3241 caccctgggc agccccgaga atgaggagca tatcctgtcc ttcgagacca tcgacaagat 3301 gcgccgcgcc agctcctact cctccctcaa ctccctcatc ggagtttcct ttaacagtgt 3361 ttacactcag atttggagag tcctgctgca cctggctgct gacccctatc cagaggtctc 3421 ggacgtggcc atgaaagtac tcaacagcat cgcctacaag gccaccgtga acgcccggcc 3481 gcagcgcgtc ctggacacct cctccctcac gcagtcggcc cccgccagcc ccaccaacaa 3541 gggcgtgcac atccaccagg cggggggctc ccctccggcg tccagcacca gcagctccag 3601 cctgaccaac gatgtggcca agcagccggt cagccgagac ttgccttctg gccggccggg 3661 caccacaggc cccgctgggg cgcagtacac ccctcactcc caccagttcc cccggacacg 3721 gaagatgttc gacaagggcc cagagcagac tgcggacgac gcggacgatg ctgctggaca 3781 caaaagtttc atctccgcca cggtgcagac ggggttctgc gactggagcg cccgctattt 3841 tgcccagccc gtcatgaaga tcccagaaga gcacgacctg gagagtcaga tccgcaagga 3901 gcgggagtgg cggttcctgc gaaacagccg tgtcaggagg caggcccagc aagtcattca 3961 gaagggcatt acgagattgg acgaccaaat atttctgaac aggaaccccg gcgtcccctc 4021 tgtggtgaaa ttccacccct tcacgccgtg catcgccgta gccgacaagg acagcatctg 4081 cttttgggac tgggagaaag gggagaagct ggattatttc cacaatggga accctcggta 4141 cacgagggtc actgccatgg agtatctgaa cggccaggac tgctcgcttc tgctgacggc 4201 cacagacgat ggtgccatca gggtctggaa gaattttgct gatttggaaa agaacccaga 4261 gatggtgacc gcgtggcagg ggctctcgga catgctgcca acgacgcgag gagctgggat 4321 ggtggtggac tgggagcagg agaccggcct cctcatgagc tcaggagacg tgcggatcgt 4381 ccggatctgg gacacagacc gtgagatgaa ggtgcaggac atccctacgg gcgcagacag 4441 ctgtgtgacg agtctgtcct gtgattccca ccgctcactc atcgtggctg gcctcggtga 4501 cggctccatc cgcgtctacg acagaaggat ggcactcagc gaatgccgcg tcatgacgta 4561 ccgggagcac acagcctggg tggtgaaggc ctccctgcag aagcgtcccg acggccacat 4621 cgtgagtgtg agcgtcaatg gagatgtgcg catctttgat ccccggatgc ctgagtcggt 4681 aaatgtgctt cagatcgtga aggggctgac ggccctggac atccaccccc aggcggacct 4741 gatcgcatgt ggctccgtca atcagttcac cgccatctac aacagcagcg gagagctcat 4801 caacaacatc aagtactacg acggcttcat gggccagcgg gtcggcgcca tcagctgcct 4861 ggccttccac ccgcactggc ctcacctggc cgtgggaagc aacgactact acatctccgt 4921 gtactcggtg gagaagcgtg tcagaCTCGA GCATCACCAT CATCACCATT GATGAGGGCC 4981 CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG 5041 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA 5101 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT 5161 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT 5221 GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC 5281 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC 5341 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT 5401 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC 5461 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC 5521 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT 5581 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 5641 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 5701 GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 5761 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 5821 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 5881 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 5941 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA 6001 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 6061 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 6121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 6181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 6241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 6301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 6361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 6421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 6481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 6541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 6601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 6661 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 6721 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG 6781 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 6841 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 6901 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 6961 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 7021 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 7081 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 7141 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 7201 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 7261 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 7321 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 7381 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 7441 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 7501 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 7561 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 7621 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 7681 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 7741 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 7801 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 7861 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 7921 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 7981 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 8041 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 8101 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 8161 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 8221 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 8281 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 8341 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 8401 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 8461 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 8521 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 8581 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 8641 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 8701 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 8761 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 8821 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 8881 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 8941 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 9001 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 9061 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 9121 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 9181 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 9241 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 9301 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 9361 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.