pLV3-CMV-Rnf26-3×FLAG-CopGFP-P

pLV3-CMV-Rnf26-3×FLAG-CopGFP-Puro plasmid

PVT95774

pLV3-CMV-Rnf26-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Rnf26(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_153762.3;8030450I18Rik
Gene lenght: 1275bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Rnf26-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Rnf26-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Rnf26-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg aggctgtcta cctggtggtg 2341 aatggggtgg gcctggtgct ggacttgctg accttgatgt tggatctcaa cttcctgctc 2401 gtgtcctccc tcctggctac cctggcctgg cttttggcct tcatctacaa tctgccacac 2461 acggtactta ccagtcttct gcacttgggt cgaggattcc tgctttcttt gctggcctta 2521 gttgaagctg tggtccgatt taccttcggg ggactgcagg ccttggggac gctcctctat 2581 agctgctact ctggcttgga gagcttaaag ctactggggc acttggcctc ccacggagct 2641 ctgaggagcc gggaattcct gaatcggggc atcctgaaca tggtctccaa tggccatgct 2701 ttgctgcgcc aggcctgtga catatgtgct attgccatga gcctggtggc ctatgtgatc 2761 aacagtctag tcaacatctg cctcatcagc actcagaact tcttctccct ggtgctggcc 2821 ctgtgggatg ctgtaacggg gcctctttgg aggatgacag acgtggtggc tgctttcctc 2881 gctcacatct ccagcagtgc agtggctatg gccattctcc tgtggacccc ctgccagcta 2941 gcactggagc tgttggcctc agccgcccgc ctgctggcca gctgtgtggt tttccatctc 3001 actggattgg tgttgctggc ttgcgtgctg gcagtgattt tgattgtgtt gcacccagaa 3061 caaaccctga ggctggccac tcaagctctc agtcagcttc atgcccgccc atcttaccac 3121 cggctttggg aggatatcgt acggctgact cgccttccac taggtctgga ggcttggcgc 3181 agagtctgga gccgcagcct acaactggcc agctggccaa atcggggagg agcaccagga 3241 gccccccaag gtggccccag gagggtgttc tcagccagga tccagccaca ggacactcct 3301 cctgaagcag aagaggaggt catcagaact gcaccagcta gaggccgaga gcaactcaac 3361 gaggacgagc ctgccgctgg gcaggaccca tggaagctgt tgaaggagca agaagagcgg 3421 aagaagtgtg ttatctgcca ggaccagagc aaaacggtgc tgcttctgcc ctgccggcac 3481 ctgtgcctgt gccaggcttg cactgagatc ctcatgcgcc accctgttta ccatcgcaac 3541 tgcccgctct gccgccgcag catcctgcaa accctcaatg tctacctcgg atccGACTAC 3601 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 3661 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3721 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 3781 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 3841 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 3901 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 3961 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4021 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4081 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4141 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4201 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4261 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4321 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4381 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4441 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4501 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4561 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4621 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 4681 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 4741 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 4801 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 4861 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 4921 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 4981 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5041 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5101 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5161 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5221 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5281 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5341 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5401 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5461 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5521 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5581 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5641 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5701 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5761 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 5821 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 5881 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 5941 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6001 ttgggcactg acaattccgt ggtgttgtcg gggaaATCAT CGTCCTTTCC Ttggctgctc 6061 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6121 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctT 6181 cgccttcgcc ctcagacgag tcggatctcc ctttggGccg cctccccgcc tggtaccttt 6241 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 6301 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 6361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 6421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 6481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 6541 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 6601 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 6661 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 6721 tgtatcttat catgtctggc tctagctaTC CCGCCCCTAA CTCCGCCCAt cccgccccta 6781 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 6841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 6901 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa 6961 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7021 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7081 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7141 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7201 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7261 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 7321 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 7381 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7441 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7501 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7561 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7621 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7681 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7741 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 7801 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7861 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7921 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7981 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8041 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8101 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 8161 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8221 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8281 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8341 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8401 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8461 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8521 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8581 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8641 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8701 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8761 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8821 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8881 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 8941 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9001 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9061 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 9121 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 9181 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 9241 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 9301 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 9361 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 9421 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 9481 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 9541 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.