pCMV-FLAG-Stk4-EGFP-Neo plasmi

pCMV-FLAG-Stk4-EGFP-Neo plasmid

PVT95756

pCMV-FLAG-Stk4-EGFP-Neo plasmid Information

Plasmid name: pCMV-FLAG-Stk4(mouse)-EGFP-Neo
Alias: NM_021420.4;Kas-2; Mst1; Ysk3
Gene lenght: 1461bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: High
Induction:

pCMV-FLAG-Stk4-EGFP-Neo plasmid Description

pCMV-FLAG-Stk4-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-FLAG-Stk4-EGFP-Neo plasmid Sequence

1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat 61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc 841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag 901 cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc 961 caccatgGAC TACAAGGACG ACGATGACAA Gatggagacc gtgcagctga ggaacccacc 1021 gcgcaggcag ctgaaaaagt tggatgaaga tagtttaacc aagcagcccg aggaagtgtt 1081 tgatgtctta gagaagcttg gagaggggtc ttatggcagc gtgtacaagg ctattcataa 1141 agagactggc cagattgttg caatcaagca agtgcccgtg gaatcggacc tgcaggagat 1201 aatcaaggaa atctccatca tgcagcagtg tgacagccct cacgtagtca agtattatgg 1261 cagctacttt aagaacacag acctgtggat tgttatggag tactgtgggg ctggttctgt 1321 atctgatatc attcggctac ggaacaagac gttaacagaa gatgaaatag ctacaatatt 1381 acaatcaact cttaagggac tagaatacct tcattttatg agaaaaatac accgagatat 1441 caaggcggga aatattttgc taaacacaga aggccatgca aagcttgcag actttggagt 1501 cgcaggtcaa cttacagata ccatggcaaa gaggaataca gtaataggga ccccgttttg 1561 gatggctcct gaagttattc aggaaattgg gtacaactgt gtggcagaca tctggtctct 1621 gggaataact gccatagaaa tggctgaagg aaagccccca tatgctgata tccatccaat 1681 gagggcaata ttcatgattc ctacaaaccc tcctcccaca ttccgtaagc cagagctgtg 1741 gtcagacaac ttcatggatt ttgtgaagca gtgtctggta aaaagcccgg agcagagagc 1801 cacagccact cagctcctac agcacccgtt tgttaagagt gccaaaggag tgtcaatatt 1861 gcgagactta attaacgaag ccatggatgt gaaactgaag cgccaggaag cccagcagcg 1921 ggaagtggac caggacgacg aggagaactc agaggaggat gaaatggatt ccggcacgat 1981 ggttcgagct gccggtgatg agatgggcac tgtccgagta gccagcacga tgagcggagg 2041 agccaatact atgattgagc acggtgacac gttgccgtcc cagctgggca ccatggtgat 2101 caacacggag gacgaggaag aggaaggaac catgaaaaga agagatgaga ccatgcagcc 2161 tgcaaaaccg tctttccttg aatactttga acaaaaagaa aaggaaaatc agatcaacag 2221 ctttggcaag aatgtatctg gctctctgaa gaattcttcc gattggaaaa tccctcagga 2281 tggagactat gagtttctca agagctggac tgtggaggac cttcagaaga ggctcttggc 2341 cctcgacccc atgatggaac aggaaatgga agagatccgg cagaagtacc ggtccaagcg 2401 gcagccaatc ctggatgcca ttgaagccaa gaagaggagg caacagaact tcggtggagg 2461 cggatcagga ggcggtggat ctatggtgag caagggcgag gagctgttca ccggggtggt 2521 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 2581 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 2641 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 2701 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 2761 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 2821 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 2881 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 2941 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3001 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3061 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3121 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3181 catggacgag ctgtacaagt aaagcggccg ctcgagtcta gagggcccgt ttaaacccgc 3241 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 3301 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 3361 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 3421 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 3481 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 3541 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 3601 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 3661 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 3721 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 3781 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 3841 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 3901 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 3961 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 4021 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 4081 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 4141 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 4201 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 4261 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 4321 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 4381 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 4441 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 4501 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 4561 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 4621 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 4681 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 4741 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 4801 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 4861 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 4921 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 4981 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 5041 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 5101 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 5161 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 5221 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 5281 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 5341 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 5401 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 5461 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 5521 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 5581 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 5641 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 5701 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 5761 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5821 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 6001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 6061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 6121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 6181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 6241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 6301 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 6361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 6421 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 6481 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 6541 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 6601 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 6661 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 6721 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 6781 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6841 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6901 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6961 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 7021 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 7081 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 7141 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 7201 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 7261 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 7321 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 7381 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 7441 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 7501 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 7561 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 7621 gcgcacattt ccccgaaaag tgccacctga cgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.