pLV2-CMV-RBM12B-3×HA-IRES-Neo

pLV2-CMV-RBM12B-3×HA-IRES-Neo plasmid

PVT96614

pLV2-CMV-RBM12B-3×HA-IRES-Neo plasmid Information

Plasmid name: pLV2-CMV-RBM12B(human)-3×HA-IRES-Neo
Alias: NM_001377960.1;MGC:33837;
Gene lenght: 3303bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-RBM12B-3×HA-IRES-Neo plasmid Description

pLV2-CMV-RBM12B-3×HA-IRES-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-RBM12B-3×HA-IRES-Neo plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggct 2821 gtagtcatcc gtttactggg gcttcctttt attgcggggc ctgtggatat tcgtcacttc 2881 ttcacgggat tgactattcc tgatggagga gtgcatataa ttggagggga aattggggag 2941 gcttttatta tttttgcaac agatgaagat gcaagacgtg ccataagtcg ttcaggaggg 3001 tttatcaagg attcatctgt agagctcttt cttagtagca aggcagaaat gcagaagact 3061 atagaaatga aaagaactga tcgtgtagga agagggcgtc caggatctgg gacatcaggg 3121 gttgacagcc tgtctaattt tattgagtct gttaaggaag aagcaagtaa ttctggatat 3181 ggctcttcaa ttaatcaaga tgctgggttt catactaatg gtacaggaca tggtaattta 3241 aggccaagaa agacaaggcc attgaaggcc gagaatcctt acttgtttct acgaggtttg 3301 ccttacctag taaatgaaga tgatgtacgt gtctttttct ctggtttgtg cgtggatgga 3361 gtaattttct taaaacatca tgatggccga aataatggtg atgccatagt aaaatttgct 3421 tcatgtgttg atgcttcagg aggtcttaaa tgtcatagaa gttttatggg ttcaagattt 3481 atagaagtaa tgcaaggatc agaacaacag tggattgagt ttggtggtaa tgcagttaag 3541 gagggtgacg ttcttaggag atctgaagaa cattctccac caagaggaat taatgataga 3601 cattttcgaa aacggtctca ttcaaaatct cccagaagaa cacgttctcg ttcccctctt 3661 ggattttatg ttcacttaaa aaatctgtcc ctcagtattg acgaaagaga tttaagaaat 3721 ttctttagag gtactgatct gactgatgaa cagattaggt ttttatataa agatgaaaat 3781 agaacaagat atgcctttgt gatgttcaag actctgaaag actataatac cgctctgagt 3841 ttacataaga ctgttttaca atatcgtcca gttcatattg atccaatttc tagaaaacaa 3901 atgctgaagt tcattgcacg ttatgaaaag aagagatcag ggtcactaga gagagatagg 3961 cccggacatg tttcacaaaa atactctcaa gaaggtaact ctggccagaa actgtgcatc 4021 tatataagaa attttccatt tgatgttaca aaagttgaag tgcagaagtt ctttgcagac 4081 tttcttcttg ctgaggatga catttacttg ctttatgatg acaaaggtgt tggtctggga 4141 gaagcattag tgaaatttaa atcagaagaa caggccatga aagctgaacg tttaaaccga 4201 cgaagattcc tagggacaga ggtgttatta agacttatat ctgaggcaca aatacaggag 4261 tttggtgtaa atttttctgt gatgtccagt gaaaaaatgc aagctcgctc acagtcacgt 4321 gagcgaggtg accattccca tttatttgac tcaaaagacc caccaatata ctcagttggt 4381 gcttttgaaa actttagaca tcagctagag gacttgaggc aactggataa cttcaagcat 4441 ccccagaggg atttccggca gcctgacagg caccctccag aagacttccg acactcctca 4501 gaggacttta ggttcccccc ggaggacttc aggcactccc cagaggactt caggcgacct 4561 agggaggaag acttcaggcg gccttctgag gaggacttca ggcgcccttg ggaggaagat 4621 ttcaggcgcc ctccggagga tgacttcagg caccctaggg aggaggactg gaggaggccc 4681 cttgaggagg actggaggcg gccactggag gaggatttca ggcggtctcc cacggaggac 4741 ttcaggcagc tccccgagga ggacttcagg caaccccctg aggaggactt aaggtggctc 4801 ccagaggaag atttcaggcg gccacctgag gaggactgga gacggccccc agaggaggac 4861 tttaggcggc ctcttcaggg agaatggagg cgaccacccg aggatgactt caggcggccc 4921 ccagaggagg atttcaggca ttcccctgag gaggacttca ggcagtcacc ccaggagcat 4981 ttccggaggc cacctcagga gcatttccgt cggccacccc cagagcattt ccggagacca 5041 cctccagagc attttaggcg gcctccccca gagcacttcc ggcggccacc cccagagcac 5101 ttcaggcggc cacccccaga gcatttcagg cgcccgcccc cggagcactt ccggagaccg 5161 ccccaggagc atttcaggcg gccgcctcag gagcatttca ggcgctcccg agaggaagat 5221 ttcaggcacc caccagatga agacttcagg ggccctcctg atgaagactt taggcaccct 5281 cctgatgagg acttcaggag cccccaggag gaagatttta gatgcccttc tgatgaggac 5341 ttcaggcagc tcccagagga agaccttagg gaagctccgg aggaggaccc tagacttcct 5401 gacaatttta gacctcctgg tgaggatttt aggagcccgc ctgatgattt tagaagtcac 5461 cgcccttttg tgaattttgg tcgcccagaa ggtggcaagt ttgattttgg aaagcataat 5521 atgggaagtt ttcctgaggg gagatttatg cctgatccaa aaataaattg tggttcaggt 5581 agagtaactc ctattaagat aatgaatctt ccatttaaag ctaatgtgaa tgaaatttta 5641 gactttttcc atggttacag aatcatacct gattcagttt cgatacagta taatgagcaa 5701 ggcttaccta caggggaagc cattgttgct atgataaact ataatgaagc tatggctgct 5761 attaaagatc taaatgatag gccagttggg ccccgaaaag ttaagttaac tttgctgctc 5821 gagTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT ATGATGTACC TGATTATGCT 5881 AGCTATCCTT ATGATGTCCC AGACTACGCA TAGactagtt ctagagcggc cgcggatccg 5941 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg 6001 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg 6061 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg 6121 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca 6181 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct 6241 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca 6301 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa 6361 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg 6421 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg 6481 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaaccatg gctgaacaag 6541 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 6601 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 6661 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 6721 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 6781 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 6841 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 6901 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 6961 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 7021 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 7081 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 7141 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 7201 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 7261 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 7321 gaacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 7381 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 7441 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 7501 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 7561 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 7621 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 7681 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 7741 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 7801 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 7861 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 7921 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca 7981 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg 8041 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc 8101 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac 8161 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact 8221 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac 8281 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc 8341 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca 8401 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct 8461 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg 8521 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt 8581 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 8641 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 8701 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 8761 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 8821 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg 8881 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 8941 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 9001 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 9061 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 9121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 9181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 9241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 9301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 9361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 9421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 9481 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 9541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 9601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 9661 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 9721 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 9781 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 9841 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 9901 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 9961 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 10021 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 10081 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 10141 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 10201 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 10261 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 10321 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 10381 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 10441 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 10501 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 10561 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 10621 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 10681 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 10741 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 10801 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 10861 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 10921 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 10981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 11041 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata 11101 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 11161 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa 11221 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt 11281 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg 11341 ttaaatatgt actacaaact tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.