pTac-GST-MAP3K20 plasmid

pTac-GST-MAP3K20 plasmid

PVT95738

pTac-GST-MAP3K20 plasmid Information

Plasmid name: pTac-GST-MAP3K20(human)
Alias: NM_016653.3;AZK; CNM6; MLK7; mlklak; MLT; MLTK;MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK
Gene lenght: 2403bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-MAP3K20 plasmid Description

pTac-GST-MAP3K20 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-MAP3K20 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc atgtcgtctc 961 tcggtgcctc ctttgtgcaa attaaatttg atgacttgca gttttttgaa aactgcggtg 1021 gaggaagttt tgggagtgtt tatcgagcca aatggatatc acaggacaag gaggtggctg 1081 taaagaagct cctcaaaata gagaaagagg cagaaatact cagtgtcctc agtcacagaa 1141 acatcatcca gttttatgga gtaattcttg aacctcccaa ctatggcatt gtcacagaat 1201 atgcttctct gggatcactc tatgattaca ttaacagtaa cagaagtgag gagatggata 1261 tggatcacat tatgacctgg gccactgatg tagccaaagg aatgcattat ttacatatgg 1321 aggctcctgt caaggtgatt cacagagacc tcaagtcaag aaacgttgtt atagctgctg 1381 atggagtatt gaagatctgt gactttggtg cctctcggtt ccataaccat acaacacaca 1441 tgtccttggt tggaactttc ccatggatgg ctccagaagt tatccagagt ctccctgtgt 1501 cagaaacttg tgacacatat tcctatggtg tggttctctg ggagatgcta acaagggagg 1561 tcccctttaa aggtttggaa ggattacaag tagcttggct tgtagtggaa aaaaacgaga 1621 gattaaccat tccaagcagt tgccccagaa gttttgctga actgttacat cagtgttggg 1681 aagctgatgc caagaaacgg ccatcattca agcaaatcat ttcaatcctg gagtccatgt 1741 caaatgacac gagccttcct gacaagtgta actcattcct acacaacaag gcggagtgga 1801 ggtgcgaaat tgaggcaact cttgagaggc taaagaaact agagcgtgat ctcagcttta 1861 aggagcagga gcttaaagaa cgagaaagac gtttaaagat gtgggagcaa aagctgacag 1921 agcagtccaa caccccgctg ctgccttcct ttgagattgg tgcatggacg gaagacgatg 1981 tgtattgttg ggttcagcag ctcgtcagaa aaggtgactc ttcagcagag atgagtgtat 2041 atgcaagctt gtttaaagaa aacaacatta cagggaagcg gctgctgctg ctggaggaag 2101 aagacctgaa agacatgggc attgtctcca aggggcatat cattcacttc aagtcagcca 2161 ttgagaaatt aacccatgat tacataaatt tgtttcactt cccaccacta attaaggact 2221 caggaggtga acctgaagaa aatgaggaaa aaatagtgaa cctggaactg gtttttggtt 2281 ttcacttgaa accaggaact ggcccacagg attgtaagtg gaaaatgtat atggagatgg 2341 atggggatga aattgcaata acctacataa aagatgtgac attcaacact aacctacctg 2401 atgcggagat tttaaagatg acaaagccac catttgtaat ggagaagtgg attgtaggaa 2461 tagcaaaaag tcagactgtg gagtgcactg tcacatatga gagtgatgtt agaactccaa 2521 aaagcactaa acatgtccat tcgattcagt ggagtagaac aaaacctcag gatgaagtga 2581 aagcagtcca acttgccatt cagacattat tcaccaattc agatggcaac cctggaagca 2641 ggtccgactc aagtgctgat tgccagtggt tagatactct gaggatgcgg cagattgcat 2701 ccaacacttc tttacagcgt tcccagagca atcctattct ggggtcaccg ttcttctcac 2761 actttgatgg ccaggattcc tacgctgctg ctgtgagacg gccccaggtg cccattaagt 2821 atcaacagat tacacctgtg aaccagtcca gaagctcgtc tcctactcag tatggactga 2881 ccaaaaactt ctcttcccta catctcaact ctagggacag tggcttttcc agtggcaata 2941 ctgacacctc ttcagagagg ggtcgatact cagacagaag caggaacaaa tatggacgtg 3001 gtagtatatc actcaattct tctcctagag gaagatacag tggaaagagt cagcattcca 3061 ctccttcaag aggaagatac cctggaaagt tctacagggt ttctcagtca gcactcaatc 3121 ctcaccagtc gcctgacttc aagagaagcc ccagggacct ccaccaaccc aacaccatac 3181 cagggatgcc tttgcaccct gagactgact caagagccag tgaagaggac agcaaagtca 3241 gcgaaggggg ctggacaaaa gtggaatacc ggaaaaagcc ccacaggcca tctcccgcca 3301 aaaccaataa agagagagcc agaggggacc accgtggatg gagaaacttt tgactcgagc 3361 ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 3421 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 3481 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 3541 agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc 3601 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 3661 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 3721 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 3781 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 3841 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 3901 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 3961 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg 4021 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 4081 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 4141 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 4201 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 4261 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 4321 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 4381 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 4441 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 4501 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 4561 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 4621 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 4681 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 4741 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 4801 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 4861 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 4921 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 4981 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 5041 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 5101 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 5161 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 5221 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 5281 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 5341 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 5401 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 5461 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 5521 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 5581 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg 5641 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc 5701 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 5761 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 5821 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 5881 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 5941 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 6001 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 6061 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 6121 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 6181 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 6241 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 6301 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 6361 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 6421 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 6481 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 6541 ataccgaaga cagctcatgt tatatcccgc cgtcaaccac catcaaacag gattttcgcc 6601 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 6661 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 6721 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 6781 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 6841 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 6901 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca 6961 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 7021 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 7081 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag 7141 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat 7201 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc 7261 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag 7321 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttggaatt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.