pLV3-CMV-PXN-EF1a-CopGFP-Puro

pLV3-CMV-PXN-EF1a-CopGFP-Puro plasmid

PVT96859

pLV3-CMV-PXN-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PXN(human)-EF1a-CopGFP-Puro
Alias: NM_001080855.3
Gene lenght: 1776bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PXN-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-PXN-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PXN-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGG ACGACCTCGA CGCCCTGCTG 2341 GCGGACTTGG AGTCTACCAC CTCCCACATC TCCAAACGGC CTGTGTTCTT GTCGGAGGAG 2401 ACCCCCTACT CATACCCAAC TGGAAACCAC ACATACCAGG AGATTGCCGT GCCACCCCCC 2461 GTCCCCCCAC CCCCGTCCAG CGAGGCCCTC AATGGCACAA TCCTTGACCC CTTAGACCAG 2521 TGGCAGCCCA GCAGCTCCCG ATTCATCCAC CAGCAGCCTC AGTCCTCATC ACCTGTGTAC 2581 GGCTCCAGTG CCAAAACTTC CAGTGTCTCC AACCCTCAGG ACAGTGTTGG CTCTCCGTGC 2641 TCCCGAGTGG GTGAGGAGGA GCACGTCTAC AGCTTCCCCA ACAAGCAGAA ATCAGCTGAG 2701 CCTTCACCCA CCGTAATGAG CACGTCCCTG GGCAGCAACC TTTCTGAACT CGACCGCCTG 2761 CTGCTGGAAC TGAACGCTGT ACAGCATAAC CCGCCAGGCT TCCCTGCAGA TGAGGCCAAC 2821 TCAAGCCCCC CGCTTCCTGG GGCCCTGAGC CCCCTCTATG GTGTCCCAGA GACTAACAGC 2881 CCCTTGGGAG GCAAAGCTGG GCCCCTGACG AAAGAGAAGC CTAAGCGGAA TGGGGGCCGG 2941 GGCCTGGAGG ACGTGCGGCC CAGTGTGGAG AGTCTCTTGG ATGAACTGGA GAGCTCCGTG 3001 CCCAGCCCCG TCCCTGCCAT CACTGTGAAC CAGGGCGAGA TGAGCAGCCC GCAGCGCGTC 3061 ACCTCCACCC AACAGCAGAC ACGCATCTCG GCCTCCTCTG CCACCAGGGA GCTGGACGAG 3121 CTGATGGCTT CGCTGTCGGA TTTCAAGATC CAGGGCCTGG AGCAAAGAGC GGATGGGGAG 3181 CGGTGCTGGG CGGCCGGCTG GCCTCGGGAC GGCGGGCGGA GCAGCCCCGG AGGGCAGGAC 3241 GAGGGAGGGT TCATGGCCCA GGGGAAGACA GGGAGCAGCT CACCCCCTGG GGGGCCCCCG 3301 AAGCCCGGGA GCCAGCTGGA CAGCATGCTG GGGAGCCTGC AGTCTGACCT GAACAAGCTG 3361 GGGGTCGCCA CAGTCGCCAA AGGAGTCTGC GGGGCCTGCA AGAAGCCCAT CGCCGGGCAG 3421 GTTGTGACCG CCATGGGGAA GACGTGGCAC CCCGAGCACT TCGTCTGCAC CCACTGCCAG 3481 GAGGAGATCG GATCCCGGAA CTTCTTCGAG CGGGATGGAC AGCCCTACTG TGAAAAGGAC 3541 TACCACAACC TCTTCTCCCC GCGCTGCTAC TACTGCAACG GCCCCATCCT GGATAAAGTG 3601 GTGACAGCCC TTGACCGGAC GTGGCACCCT GAACACTTCT TCTGTGCACA GTGTGGAGCC 3661 TTCTTTGGTC CCGAAGGGTT CCACGAGAAG GACGGCAAGG CCTACTGTCG CAAGGACTAC 3721 TTCGACATGT TCGCACCCAA GTGTGGCGGC TGCGCCCGGG CCATCCTGGA GAACTATATC 3781 TCAGCCCTCA ACACGCTGTG GCATCCTGAG TGCTTTGTGT GCCGGGAATG CTTCACGCCA 3841 TTCGTGAACG GCAGCTTCTT CGAGCACGAC GGGCAGCCCT ACTGTGAGGT GCACTACCAC 3901 GAGCGGCGCG GCTCGCTGTG TTCTGGCTGC CAGAAGCCCA TCACCGGCCG CTGCATCACC 3961 GCCATGGCCA AGAAGTTCCA CCCCGAGCAC TTCGTCTGTG CCTTCTGCCT CAAGCAGCTC 4021 AACAAGGGCA CCTTCAAGGA GCAGAACGAC AAGCCTTACT GTCAGAACTG CTTCCTCAAG 4081 CTCTTCTGCT AGggatccCT GCGATATCCA GCgcggccgc Gaaggatctg cgatcgctcc 4141 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 4201 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4261 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4321 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4381 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4441 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4501 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4561 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4621 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4681 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 4741 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4801 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4861 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4921 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4981 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 5041 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 5101 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 5161 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 5221 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 5281 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 5341 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 5401 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 5461 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5521 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5581 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5641 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5701 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5761 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5821 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5881 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5941 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 6001 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 6061 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 6121 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 6181 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 6241 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6301 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6361 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6421 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6481 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 6541 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6601 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc 6661 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat 6721 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa 6781 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 6841 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 6901 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 6961 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 7021 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 7081 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 7141 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 7201 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 7261 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 7321 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt 7381 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 7441 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 7501 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 7561 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 7621 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 7681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 7741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 7801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 7861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 7921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 7981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 8041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 8101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 8161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 8221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 8281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 8341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 8401 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 8461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 8521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 8581 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 8641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 8701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 8761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 8821 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 8881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 8941 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 9001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 9061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 9121 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 9181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 9241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 9301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 9361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 9421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 9481 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 9541 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 9601 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 9661 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 9721 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 9781 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 9841 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 9901 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 9961 cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.