pLV3-CMV-Egr1-3×FLAG-CopGFP-Pu

pLV3-CMV-Egr1-3×FLAG-CopGFP-Puro plasmid

PVT95624

pLV3-CMV-Egr1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Egr1(rat)-3×FLAG-CopGFP-Puro
Alias: NM_012551.3;Krox-24; Ngf1; Ngfi; NGFI-A; zif-268
Gene lenght: 1608bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-Egr1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Egr1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Egr1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg cagcggccaa ggccgagatg 2341 caattgatgt ctccgctgca gatctctgac ccgttcggct cctttcctca ctcacccacc 2401 atggacaact accccaaact ggaggagatg atgctgctga gcaacggggc tccccagttc 2461 ctcggtgctg ccggaacccc agagggcagc ggcggcaata acagcagcag cagcagcagc 2521 agcagcagcg ggggcggtgg tgggggcggc agcaacagcg gcagcagcgc tttcaatcct 2581 caaggggagc cgagcgaaca accctacgag cacctgacca cagagtcctt ttctgacatc 2641 gctctgaata acgagaaggc gctggtggag acaagttatc ccagccaaac tacccggttg 2701 cctcccatca cctatactgg ccgcttctcc ctggagcctg cacccaacag tggcaacact 2761 ttgtggcctg aacccctttt cagcctagtc agtggccttg tgagcatgac caaccctcca 2821 acctcttcat cctcagcgcc ttctccagct gcttcatcgt cttcctctgc ctcccagagc 2881 ccacccctga gctgtgccgt gccgtccaac gacagcagtc ccatttactc agctgcaccc 2941 acctttccta ctcccaacac tgacattttt cctgagcccc aaagccaggc ctttcctggc 3001 tctgcaggca cagccttgca gtacccgcct cctgcctacc ctgccaccaa gggtggtttc 3061 caggttccca tgatccctga ctatctgttt ccacaacaac agggagacct gagcctgggc 3121 accccagacc agaagccctt ccagggtctg gagaaccgta cccagcagcc ttcgctcact 3181 ccactatcca ctatcaaagc cttcgccact cagtcgggct cccaggactt aaaggctctt 3241 aataacacct accagtccca actcatcaaa cccagccgca tgcgcaagta ccccaaccgg 3301 cccagcaaga caccccccca tgaacgcccg tatgcttgcc ctgttgagtc ctgcgatcgc 3361 cgcttttctc gctcggatga gcttacacgc cacatccgca tccatacagg ccagaagccc 3421 ttccagtgtc gaatctgcat gcgtaatttc agtcgtagtg accaccttac cacccacatc 3481 cgcacccaca caggcgagaa gccttttgcc tgtgacattt gtgggagaaa gtttgccagg 3541 agtgatgaac gcaagaggca taccaaaatc cacttaagac agaaggacaa gaaagcagac 3601 aaaagtgtcg tggcctcctc agctgcctct tccctctctt cctacccatc cccagtggct 3661 acctcctacc catcccccgc caccacctca tttccatccc cagtgcccac ctcttactcc 3721 tctccgggct cctctaccta cccgtctcct gcacacagtg gcttcccatc gccctcggtg 3781 gccaccacct atgcctccgt cccacctgct ttccctgccc aggtcagcac cttccagtct 3841 gcaggggtca gcaactcctt cagcacctca acgggtcttt cagacatgac agcaaccttt 3901 tctcctagga caattgaaat ttgcggatcc GACTACAAAG ACCATGACGG TGATTATAAA 3961 GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag gatctgcgat 4021 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 4081 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 4141 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 4201 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 4261 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 4321 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 4381 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 4441 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 4501 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 4561 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 4621 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 4681 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 4741 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 4801 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 4861 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 4921 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 4981 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 5041 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 5101 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 5161 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 5221 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 5281 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 5341 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 5401 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 5461 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 5521 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 5581 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 5641 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 5701 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 5761 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5821 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 5881 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 5941 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 6001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 6061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 6121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 6181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 6241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6361 ttgtcgggga aATCATCGTC CTTTCCTtgg ctgctcgcct gtgttgccac ctggattctg 6421 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6481 ggcctgctgc cggctctgcg gcctcttccg cgtctTcgcc ttcgccctca gacgagtcgg 6541 atctcccttt ggGccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 6601 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6661 acgaaGataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6721 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6781 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6841 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6901 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6961 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 7021 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 7081 gctaTCCCGC CCCTAACTCC GCCCAtcccg cccctaactc cgcccagttc cgcccattct 7141 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 7201 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 7261 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7321 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7381 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7441 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7501 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7561 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7621 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7681 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7741 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7801 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7861 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7921 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7981 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 8041 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 8101 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 8161 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 8221 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8281 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 8341 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8401 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8461 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8521 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8581 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8641 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8701 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8761 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8821 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8881 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8941 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 9001 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 9061 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 9121 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 9181 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 9241 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9421 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9481 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9541 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9601 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9661 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9721 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9781 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9841 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.