pCMV-Vars2-3×HA-Neo plasmid

pCMV-Vars2-3×HA-Neo plasmid

PVT95489

pCMV-Vars2-3×HA-Neo plasmid Information

Plasmid name: pCMV-Vars2(mouse)-3×HA-Neo
Alias: NM_175137.51190004I24Rik; mKIAA1885; Vars2l
Gene lenght: 3180bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Vars2-3×HA-Neo plasmid Description

pCMV-Vars2-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Vars2-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCg ccaccatgcc tcatttgcct 721 ctggcctctt tccggccacc gctctggggg ctgaggccct catggggtct ttccaggccc 781 caggcacttt gtacacagcc ggagccccac ggctcccctg tctctcggag gaaccgtgaa 841 gccaaacaga agcgcctgcg cgagaaacag gcggccctgg aggctgggct agctgagaag 901 agcaagatac ctgcagtgcc cactaaggcc tggagtcaca aggaggtagt attgtatgaa 961 atccccaccg ggccaggtga aaagaaagat gtttctgggc ccctgcctcc tgcttacagt 1021 ccccagtatg ttgaagcggc ctggtaccag tggtgggtgc gagagggctt cttcaaacca 1081 gagtatcagg caaggctgcc ccaagctaca ggggagacct tttccatgtg tatcccacct 1141 cccaatgtca ccggctccct gcacattggc catgcactca cagtggcgat ccaggacgca 1201 cttgtgcgct ggcaccggat gcgtggggat cgcgtgctgt ggatccccgg gtctgatcac 1261 gcaggaattg ctacacaggc catggtggag aagcagctat ggaaggagca gagggtgcgg 1321 agacatgagc tgagccggga agacttcctc agggccgtgt ggcagtggaa acatgagaaa 1381 ggaggggaga tctatgaaca gctgtgtgct ctgggtgcct ctctggactg ggatcgagag 1441 tgtttcacca tggatgccgg ctcctcagca gctgtgacgg aagctttcgt gcgactctac 1501 aactcgggat tgttgtaccg gaaccgccag cttgtcaact ggtcatgtac tttgcgatcg 1561 gccatctcag acattgaggt ggaaagccgg cctctgcctg gccgcacggt gctgcagctg 1621 cccggatgcc caacccctgt gtcttttggg ctccttgctt ctgttgcttt ccctgtggat 1681 ggagagcctg acacagagat tgtggtggga accaccaggc cagagaccct gcctggagat 1741 gtggctgtgg ccgttcatcc agatgacccg agatacacgc atttacatgg gcgacagctc 1801 cgtcatcctt tgacaggaca gcttctcccc ctcatcacag acaccactgt tcagccacat 1861 gtgggcacag gggcagtgaa ggtgactcct gctcacagtc caatagatgc tgagataggg 1921 acccggcatg gcctgacccc actgagtgtc attgcagagg atgggaccat gacatccctc 1981 tgtggagact ggctgcaggg ccttcatcga tttgtggccc gggaaaagat catgtgcaca 2041 ctgagggagc agggcctatt ccggggccta caggagcacc ccatggtact gcccatctgc 2101 agccgttccg gggacgtggt ggagtacctg ctgaagagcc agtggtttgt ccgctgccag 2161 gaaatggggg acctagctgc caaggctgtg gagtcagggg ctctggagct ctggccctcc 2221 ttccaccaga agagctggca acactggttt gcccacattg gggactggtg tgtctcccgg 2281 cagctgtggt ggggccatca gattccagcc taccgggtca tcggggagaa tgcagaggat 2341 gacaggaagg aatgttgggt ggtcggacgg tcagaggctg aggccagagc ggtagcagca 2401 aaacggacag ggagaccaga ggcagagctg accctggaga gggaccctga cgtcctggac 2461 acttggttct cttcggctct tttccccttt tctgccctgg gttggccgcg agagacgcca 2521 gaccttgctc acttctaccc cctcaccctc ttggaaacgg gtagtgacct gctgatgttc 2581 tgggtgggcc gcatggtcat gctggggacc cagctcactg ggcagctccc cttcagcaag 2641 gtgcttcttc actccatggt tcgggacagg cagggccgga agatgagcaa gtccctgggg 2701 aacgtgctag acccacggga catcattagc gggcaggagc tgcaggtgct gcaggccaag 2761 ctgagagacg gtaacttgga ccctggagag ctcgccgttg cagccgcagc acagaaaaag 2821 gactttcctt acgggatccc tgagtgtggg acggatgccc tgagatttgc actgtgctcc 2881 catggaatcc tggggggcga cctgcacctg tctgtctctg aggtcctgaa ctaccgccat 2941 ttctgcaaca agctctggaa tgccctgcgc tttgtcctcc gtgccctggg ggataacttc 3001 gtgccccaac cagcagagga ggtaaccccc tcctctccca tggatgcctg gattctaagc 3061 cgcctggcct ttgcagccag cgaatgtgag agaggcttcc tgagtcggga gctttcgctt 3121 gtcacccaca ccctgtatca tttctggctc cacaatctct gtgatgtcta cctggaagca 3181 gtgaagccag tgttgtcgag cgtgccccgt cccccaggac cccctcaggt cctgttttcc 3241 tgtgcagacg tgggtctacg cctccttgcc ccgctgatgc ccttcctggc cgaggagctg 3301 tggcagaggc tgccccccag gccaggcggc ccccttgccc ccagcatctg tgtggccccc 3361 taccccagca cccgcagcct ggagttctgg cgccagccag agctggagcg ctgcttctcc 3421 agagtccaag aggttgtaca ggcattgcgg gcgctccgag ccacctacca gctcacaaaa 3481 gcccgacccc aagtgctgct gcagtgctcg gacccaggag aacaaggcct agtccagccc 3541 tttctggagc ctctgggcat cctgagccac tgcggggctg taggattcct gtccccaggg 3601 gcagcagctc cctcagggtg ggccctgacc ccactgggtg acaccatgaa gatctacatg 3661 gagctgcagg gcctggtgga cccccagagc cagctgcccc ggctcactgc ccgaaggcag 3721 aagttacaga agcagcttga tgacctgttg aaccggacca tgtcagaggg tcttgcagag 3781 aggcagcaga ggatttcctc cctccatctg gaattgtcaa agctggacca ggcagcctcc 3841 tacctacagc aactgatgga tgaggctccc agtgccaggg agctcCTCGA GTACCCATAC 3901 GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT 3961 GATGTCCCAG ACTACGCATA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA 4021 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 4081 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 4141 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 4201 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 4261 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 4321 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 4381 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 4441 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 4501 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 4561 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG 4621 GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC 4681 GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG 4741 TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC 4801 CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC 4861 CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG 4921 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 4981 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 5041 CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 5101 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 5161 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 5221 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA 5281 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT 5341 GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 5401 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC 5461 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC 5521 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 5581 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 5641 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC 5701 CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG 5761 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 5821 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 5881 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 5941 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 6001 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 6061 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 6121 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC 6181 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 6241 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 6301 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT 6361 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG 6421 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT 6481 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC 6541 GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 6601 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 6661 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 6721 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 6781 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 6841 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 6901 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 6961 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 7021 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 7081 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 7141 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 7201 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 7261 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 7321 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 7381 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 7441 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC 7501 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 7561 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 7621 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 7681 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 7741 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 7801 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 7861 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 7921 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 7981 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 8041 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 8101 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 8161 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 8221 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 8281 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 8341 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 8401 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 8461 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 8521 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 8581 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 8641 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 8701 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 8761 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 8821 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 8881 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 8941 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 9001 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC 9061 CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA 9121 TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC 9181 AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.