pLV2-CMV-ORF3A-IRES-ZsGreen1 p

pLV2-CMV-ORF3A-IRES-ZsGreen1 plasmid

PVT91221

pLV2-CMV-ORF3A-IRES-ZsGreen1 plasmid Information

Plasmid name: pLV2-CMV-ORF3A(SARS-CoV-2)-IRES-ZsGreen1
Alias:
Gene lenght: 825bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: COVID-19 Virus
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pIRES2-EGFP.P3'
Copy number: High
Induction:

pLV2-CMV-ORF3A-IRES-ZsGreen1 plasmid Description

pLV2-CMV-ORF3A-IRES-ZsGreen1 It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-ORF3A-IRES-ZsGreen1 plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcGC CACCatggat 2821 ttgtttatga gaatcttcac aattggaact gtaactttga agcaaggtga aatcaaggat 2881 gctactcctt cagattttgt tcgcgctact gcaacgatac cgatacaagc ctcactccct 2941 ttcggatggc ttattgttgg cgttgcactt cttgctgttt ttcagagcgc ttccaaaatc 3001 ataaccctca aaaagagatg gcaactagca ctctccaagg gtgttcactt tgtttgcaac 3061 ttgctgttgt tgtttgtaac agtttactca caccttttgc tcgttgctgc tggccttgaa 3121 gccccttttc tctatcttta tgctttagtc tacttcttgc agagtataaa ctttgtaaga 3181 ataataatga ggctttggct ttgctggaaa tgccgttcca aaaacccatt actttatgat 3241 gccaactatt ttctttgctg gcatactaat tgttacgact attgtatacc ttacaatagt 3301 gtaacttctt caattgtcat tacttcaggt gatggcacaa caagtcctat ttctgaacat 3361 gactaccaga ttggtggtta tactgaaaaa tgggaatctg gagtaaaaga ctgtgttgta 3421 ttacacagtt acttcacttc agactattac cagctgtact caactcaatt gagtacagac 3481 actggtgttg aacatgttac cttcttcatc tacaataaaa ttgttgatga gcctgaagaa 3541 catgtccaaa ttcacacaat cgacggttca tccggagttg ttaatccagt aatggaacca 3601 atttatgatg aaccgacgac gactactagc gtgcctttgt agctcgagac tagttctaga 3661 gcggccgcgg atcccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct 3721 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg 3781 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt 3841 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg 3901 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac 3961 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg 4021 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctcacct 4081 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg 4141 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg 4201 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggcccagt 4261 ccaagcacgg cctgaccaag gagatgacca tgaagtaccg catggagggc tgcgtggacg 4321 gccacaagtt cgtgatcacc ggcgagggca tcggctaccc cttcaagggc aagcaggcca 4381 tcaacctgtg cgtggtggag ggcggcccct tgcccttcgc cgaggacatc ttgtccgccg 4441 ccttcatgta cggcaaccgc gtgttcaccg agtaccccca ggacatcgtc gactacttca 4501 agaactcctg ccccgccggc tacacctggg accgctcctt cctgttcgag gacggcgccg 4561 tgtgcatctg caacgccgac atcaccgtga gcgtggagga gaactgcatg taccacgagt 4621 ccaagttcta cggcgtgaac ttccccgccg acggccccgt gatgaagaag atgaccgaca 4681 actgggagcc ctcctgcgag aagatcatcc ccgtgcccaa gcagggcatc ttgaagggcg 4741 acgtgagcat gtacctgctg ctgaaggacg gtggccgctt gcgctgccag ttcgacaccg 4801 tgtacaaggc caagtccgtg ccccgcaaga tgcccgactg gcacttcatc cagcacaagc 4861 tgacccgcga ggaccgcagc gacgccaaga accagaagtg gcacctgacc gagcacgcca 4921 tcgcctccgg ctccgccttg ccctgaacgc gtctggaaca atcaacctct ggattacaaa 4981 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 5041 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 5101 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 5161 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 5221 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 5281 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 5341 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 5401 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 5461 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 5521 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa 5581 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag 5641 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa 5701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag 5761 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag 5821 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct 5881 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag 5941 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag 6001 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc 6061 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg 6121 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca 6181 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg 6241 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 6301 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 6361 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 6421 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta 6481 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 6541 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 6601 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 6661 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 6721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 6781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 6841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 6901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 6961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 7021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 7081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 7141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 7201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 7261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 7321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 7381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 7441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 7501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 7561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 7621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 7681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 7741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 7801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 7861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 7921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 7981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 8041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 8101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 8161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 8221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 8281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 8341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 8401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 8461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 8521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 8581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 8641 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat 8701 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 8761 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 8821 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat 8881 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt 8941 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga 9001 aattgtattt gttaaatatg tactacaaac ttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.