pLV3-CMV-HADHA-3×FLAG-CopGFP-P

pLV3-CMV-HADHA-3×FLAG-CopGFP-Puro plasmid

PVT95475

pLV3-CMV-HADHA-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HADHA(human)-3×FLAG-CopGFP-Puro
Alias: NM_000182.5;ECHA; GBP; HADH; LCEH; LCHAD; MTPA;TP-ALPHA
Gene lenght: 2289bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HADHA-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-HADHA-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HADHA-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg tggcctgccg ggcgattggc 2341 atcctcagcc gcttttctgc cttcaggatc ctccgctccc gaggttatat atgccgcaat 2401 tttacagggt cttctgcttt gctgaccaga acccatatta actatggagt caaaggggat 2461 gtggcagttg ttcgaattaa ctctcccaat tcaaaggtaa atacactgag taaagagcta 2521 cattcagagt tctcagaagt tatgaatgaa atctgggcta gtgatcaaat cagaagtgcc 2581 gtccttatct catcaaagcc aggctgcttt attgcaggtg ctgatatcaa catgttagcc 2641 gcttgcaaga cccttcaaga agtaacacag ctatcacaag aagcacagag aatagttgag 2701 aaacttgaaa agtccacaaa gcctattgtg gctgccatca atggatcctg cctgggagga 2761 ggacttgagg ttgccatttc atgccaatac agaatagcaa caaaagacag aaaaacagta 2821 ttaggtaccc ctgaagtttt gctgggggcc ttaccaggag caggaggcac acaaaggctg 2881 cccaaaatgg tgggtgtgcc tgctgctttg gacatgatgc tgactggtag aagcattcgt 2941 gcagacaggg caaagaaaat gggactggtt gaccaactgg tggaacccct gggaccagga 3001 ctaaaacctc cagaggaacg gacaatagaa tacctagaag aagttgcaat tacttttgcc 3061 aaaggactag ctgataagaa gatctctcca aagagagaca agggattggt ggaaaaattg 3121 acagcgtatg ccatgactat tccatttgtc aggcaacagg tttacaaaaa agtggaagaa 3181 aaagtgcgaa agcagactaa aggcctttat cctgcacctc tgaaaataat tgatgtggta 3241 aagactggaa ttgagcaagg gagtgatgcc ggttatctct gtgaatctca gaaatttgga 3301 gagcttgtaa tgaccaaaga atcaaaggcc ttgatgggac tctaccatgg tcaggtcctg 3361 tgcaagaaga ataaatttgg agctccacag aaggatgtta agcatctggc tattcttggt 3421 gcagggctga tgggagcagg catcgcccaa gtctccgtgg ataaggggct aaagactata 3481 cttaaagatg ccaccctcac tgcgctagac cgaggacagc aacaagtgtt caaaggattg 3541 aatgacaaag tgaagaagaa agctctaaca tcatttgaaa gggattccat cttcagcaac 3601 ttgactgggc agcttgatta ccaaggtttt gaaaaggccg acatggtgat tgaagctgtg 3661 tttgaggacc ttagtcttaa gcacagagtg ctaaaggaag tagaagcggt gattccagat 3721 cactgtatct ttgccagtaa cacatctgct ctcccaatca gtgaaatcgc tgctgtcagc 3781 aaaagacctg agaaggtgat tggcatgcac tacttctctc ccgtggacaa gatgcagctg 3841 ctggagatta tcacgaccga gaaaacttcc aaagacacca gtgcttcagc tgtagcagtt 3901 ggtctcaagc aggggaaggt catcattgtg gttaaggatg gacctggctt ctatactacc 3961 aggtgtcttg cgcccatgat gtctgaagtc atccgaatcc tccaggaagg agttgacccg 4021 aagaagctgg attccctgac cacaagcttt ggctttcctg tgggtgccgc cacactggtg 4081 gatgaagttg gtgtggatgt agcgaaacat gtggcggaag atctgggcaa agtctttggg 4141 gagcggtttg gaggtggaaa cccagaactg ctgacacaga tggtgtccaa gggcttccta 4201 ggtcgtaaat ctgggaaggg cttttacatc tatcaggagg gtgtgaagag gaaggatttg 4261 aattctgaca tggatagtat tttagcgagt ctgaagctgc ctcctaagtc tgaagtctca 4321 tcagacgaag acatccagtt ccgcctggtg acaagatttg tgaatgaggc agtcatgtgc 4381 ctgcaagagg ggatcttggc cacacctgca gagggagaca tcggagccgt ctttgggctt 4441 ggcttcccgc cttgtctggg agggcctttc cgctttgtgg atctgtatgg cgcccagaag 4501 atagtggacc ggctcaagaa atatgaagct gcctatggaa aacagttcac cccatgccag 4561 ctgctagctg accatgctaa cagccctaac aagaagttct accagggatc cGACTACAAA 4621 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag 4681 cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 4741 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 4801 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 4861 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 4921 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 4981 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 5041 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 5101 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 5161 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 5221 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 5281 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 5341 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 5401 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 5461 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 5521 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 5581 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 5641 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 5701 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 5761 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 5821 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 5881 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 5941 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 6001 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 6061 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 6121 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 6181 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 6241 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 6301 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 6361 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 6421 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 6481 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 6541 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 6601 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 6661 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 6721 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 6781 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 6841 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 6901 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6961 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 7021 ggcactgaca attccgtggt gttgtcgggg aaATCATCGT CCTTTCCTtg gctgctcgcc 7081 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 7141 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctTcgc 7201 cttcgccctc agacgagtcg gatctccctt tggGccgcct ccccgcctgg tacctttaag 7261 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 7321 ggaagggcta attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct 7381 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 7441 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 7501 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 7561 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 7621 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 7681 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 7741 atcttatcat gtctggctct agctaTCCCG CCCCTAACTC CGCCCAtccc gcccctaact 7801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 7861 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 7921 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 7981 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 8041 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 8101 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 8161 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 8221 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 8281 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 8341 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 8401 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 8461 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 8521 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 8581 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 8641 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 8701 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 8761 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 8821 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 8881 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8941 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 9001 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 9061 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 9121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 9181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 9241 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 9301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 9361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 9421 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 9481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 9541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 9601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 9661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 9721 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 9781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 9841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 9901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 9961 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 10021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 10081 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 10141 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 10201 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 10261 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 10321 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 10381 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 10441 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 10501 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 10561 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.