pLV3-CMV-KLF9-3×FLAG-EF1a-CopG

pLV3-CMV-KLF9-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT95377

pLV3-CMV-KLF9-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-KLF9(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_001206.4;BTEB; BTEB1
Gene lenght: 732bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KLF9-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-KLF9-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-KLF9-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgtccgc ggccgcctac atggacttcg tggctgccca 2341 gtgtctggtt tccatttcga accgcgctgc ggtgccggag catggggtcg ctccggacgc 2401 cgagcggctg cgactacctg agcgcgaggt gaccaaggag cacggtgacc cgggggacac 2461 ctggaaggat tactgcacac tggtcaccat cgccaagagc ttgttggacc tgaacaagta 2521 ccgacccatc cagaccccct ccgtgtgcag cgacagtctg gaaagtccag atgaggatat 2581 gggatccgac agcgacgtga ccaccgaatc tgggtcgagt ccttcccaca gcccggagga 2641 gagacaggat cctggcagcg cgcccagccc gctctccctc ctccatcctg gagtggctgc 2701 gaaggggaaa cacgcctccg aaaagaggca caagtgcccc tacagtggct gtgggaaagt 2761 ctatggaaaa tcctcccatc tcaaagccca ttacagagtg catacaggtg aacggccctt 2821 tccctgcacg tggccagact gccttaaaaa gttctcccgc tcagacgagc tgacccgcca 2881 ctaccggacc cacactgggg aaaagcagtt ccgctgtccg ctgtgtgaga agcgcttcat 2941 gaggagtgac cacctcacaa agcacgcccg gcggcacacc gagttccacc ccagcatgat 3001 caagcgatcg aaaaaggcgc tggccaacgc tttgggatcc GACTACAAAG ACCATGACGG 3061 TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAgc ggccgcGaag 3121 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 3181 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa 3241 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 3301 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 3361 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg 3421 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc 3481 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc 3541 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact 3601 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc 3661 tacgctagac gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg 3721 ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac 3781 ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag 3841 cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag 3901 cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat 3961 cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg 4021 ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt 4081 caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa 4141 cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt 4201 cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg 4261 gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat 4321 cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc 4381 gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag 4441 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctGCCA CCatggctga 4501 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4561 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4621 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 4681 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4741 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4801 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4861 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 4921 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 4981 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 5041 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5101 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5161 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5221 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5281 cttctgaGTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 5341 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 5401 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 5461 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 5521 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 5581 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 5641 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT 5701 TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 5761 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 5821 TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC 5881 GCggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 5941 gaaaaggggg gactggaagg gctaattcac tcccaacgaa Gataagatct gctttttgct 6001 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6061 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 6121 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 6181 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 6241 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 6301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 6361 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 6421 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 6481 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact 6541 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 6601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 6661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 6721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 6781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 6841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 6901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 6961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 7021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 7081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 7141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 7201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 7261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7381 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 7441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 7501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 7561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 7621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 7681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 7741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 7801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 7861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 7921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 7981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 8041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 8101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 8161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 8221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 8281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 8341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 8401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 8461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 8521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 8581 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 8641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 8701 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 8761 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 8821 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 8881 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 8941 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 9001 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt 9061 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 9121 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 9181 cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.