pBiFC-CMV-HA-Ntrk2-VC155 plasm

pBiFC-CMV-HA-Ntrk2-VC155 plasmid

PVT94791

pBiFC-CMV-HA-Ntrk2-VC155 plasmid Information

Plasmid name: pBiFC-CMV-HA-Ntrk2(mouse)-VC155
Alias: NM_001025074.3;GP145-TrkB/GP95-TrkB; Tkrb; trk-B; trkB
Gene lenght: 2463bp
Host: Mammalian cells
Use(s): Bimolecular fluorescence
Fragment type: BIFC
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: SV40 intron-F1
Reverse primer:
Copy number: High
Induction:

pBiFC-CMV-HA-Ntrk2-VC155 plasmid Description

pBiFC-CMV-HA-Ntrk2-VC155 This is a plasmid suitable for bimolecular fluorescence in mammalian cells.

pBiFC-CMV-HA-Ntrk2-VC155 plasmid Sequence

1 ATGTACCCAT ACGATGTTCC AGATTACGCT CTTATGGCCA TGGAGGCCCG AATTCGTatg 61 tcgccctggc tgaagtggca tggacccgcc atggcgcggc tctggggctt atgcctgctg 121 gtcttgggct tctggagggc ctctctcgcc tgcccgacgt cctgcaaatg cagttccgct 181 aggatttggt gtactgagcc ttctccaggc atcgtggcat tcccgaggtt ggaacctaac 241 agcgttgacc cggagaacat cacggaaatt ctcattgcaa accagaaaag gctagaaatc 301 atcaatgaag atgacgttga agcttacgtg gggctgagaa accttacaat tgtggattcc 361 ggcttaaagt ttgtggctta caaagcgttt ctgaaaaaca gcaacctgcg gcacataaat 421 ttcacacgaa acaagctgac gagtttgtcc aggagacatt tccgccacct tgacttgtct 481 gacctgatcc tgacgggtaa tccgttcacg tgctcctgcg acatcatgtg gctcaagact 541 ctccaggaga ctaaatccag ccccgacact caggatttgt actgcctcaa tgagagcagc 601 aagaacatgc ccctggcgaa cctgcagata cccaattgtg gtctgccatc tgcacgtctg 661 gctgctccta acctcaccgt ggaggaagga aagtctgtga ccctttcctg cagtgtgggg 721 ggtgacccac tccccacctt gtactgggac gttgggaatt tggtttccaa gcacatgaat 781 gaaacaagcc acacacaggg ctccttaagg ataacgaaca tttcatctga tgacagtgga 841 aagcaaatct cttgtgtggc agaaaacctt gtaggagaag atcaagattc tgtgaacctc 901 actgtgcatt ttgcgccaac tatcacgttt ctcgagtctc caacctcaga tcaccactgg 961 tgcattccat tcactgtgag aggcaacccc aagcctgcgc ttcagtggtt ctacaatggg 1021 gccatactga atgagtccaa gtacatctgt actaagatcc acgtcaccaa tcacacggag 1081 taccatggct gcctccagct ggataacccc actcatatga ataacggaga ctacaccctg 1141 atggccaaga acgagtatgg gaaggatgag agacagatct ccgctcactt catgggccgg 1201 cctggagtcg actacgagac aaacccaaat taccctgaag tcctctatga agactggacc 1261 acgccaactg acattgggga tactacgaac aaaagtaatg aaatcccctc cacggatgtt 1321 gctgaccaaa gcaatcggga gcatctctcg gtctatgccg tggtggtgat tgcatctgtg 1381 gtgggattct gcctgctggt gatgttgctc ctgctcaagt tggcgagaca ttccaagttt 1441 ggcatgaaag gcccagcttc ggtcatcagc aacgacgatg actctgccag ccccctccac 1501 cacatctcca atgggagtaa cactccatct tcttcggagg gcggtcccga cgctgtcatt 1561 attggaatga ccaagattcc tgttattgaa aacccccagt actttggcat caccaacagt 1621 cagctcaagc cagacacatt tgttcagcat atcaagagac acaacatcgt tctgaagagg 1681 gaacttgggg aaggagcctt cgggaaagtt ttccttgccg agtgctacaa cctctgccca 1741 gagcaggata agatcctggt ggctgtgaag acgctgaagg acgccagcga caatgcacgc 1801 aaggactttc atcgggaagc tgagctgctg accaacctcc agcacgagca cattgtcaag 1861 ttctacggtg tctgtgtgga gggcgaccca ctcatcatgg tctttgagta catgaagcac 1921 ggggacctca acaagttcct tagggcacac gggcccgacg cagtgctgat ggcagagggt 1981 aacccgccca cagagctgac gcagtcgcag atgctgcaca tcgctcagca aatcgcagca 2041 ggtatggtct acctggcgtc ccaacacttt gtgcaccgtg acctggccac ccggaactgc 2101 ctggtgggag agaacctgct ggtgaaaatt ggggactttg ggatgtcccg agatgtgtac 2161 agcaccgact actatcgggt cggtggccac acaatgttgc ccatccgatg gatgcctcca 2221 gagagcatca tgtacaggaa attcaccacc gagagcgacg tctggagcct gggcgttgtg 2281 ttgtgggaga tcttcaccta cggcaagcag ccctggtatc agctatcgaa caatgaggtg 2341 atagagtgca tcacccaggg aagagtcctt cagcggcctc gaacgtgtcc ccaggaggtg 2401 tatgagctca tgcttggatg ctggcagcgg gaaccacaca cccggaagaa catcaagagc 2461 atccacaccc tccttcagaa cttggccaag gcatctcccg tctacctgga tatcctaggc 2521 ggtggaggcg gatcaggagg cggtggatct GGAGGCGGTG GATCAGAATT CCGTCCGGCG 2581 TGCAAAATCC CGAACGACCT GAAACAGAAA GTCATGAACC ACGACAAGCA GAAGAACGGC 2641 ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC 2701 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC 2761 CTGAGCTACC AGTCCAAACT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG 2821 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGCG 2881 GCCGCGGGGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 2941 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 3001 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 3061 AGGGGGAGGT GTGGGAGGTT TTTTCGGATC CTCTAGAGTC GATCTGCAGG CATGCTAGCT 3121 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 3181 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 3241 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 3301 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 3361 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 3421 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 3481 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 3541 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 3601 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 3661 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 3721 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 3781 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 3841 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 3901 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 3961 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 4021 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 4081 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 4141 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 4201 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 4261 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 4321 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 4381 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 4441 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 4501 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 4561 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 4621 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 4681 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 4741 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 4801 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 4861 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 4921 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 4981 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 5041 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 5101 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 5161 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 5221 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 5281 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA 5341 CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC 5401 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG 5461 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA 5521 TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT 5581 ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC 5641 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT 5701 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGTTCGAGC 5761 TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA 5821 ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA 5881 CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC 5941 AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT 6001 GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT 6061 TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC 6121 GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT 6181 GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA 6241 TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC 6301 AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT 6361 CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG AAAGTTAACT 6421 GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG GTGCAAATCA 6481 AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA GTGTTACTTC 6541 TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACC

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.