pCMV-YAP1-P2A-WWTR1-Neo plasmi

pCMV-YAP1-P2A-WWTR1-Neo plasmid

PVT95343

pCMV-YAP1-P2A-WWTR1-Neo plasmid Information

Plasmid name: pCMV-YAP1(human)-P2A-WWTR1(human)-Neo
Alias: NM_001130145.3;COB1; YAP; YAP-1; YAP2; YAP65; YKI/NM_015472.6;TAZ
Gene lenght: 1512/1203bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-YAP1-P2A-WWTR1-Neo plasmid Description

pCMV-YAP1-P2A-WWTR1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-YAP1-P2A-WWTR1-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gatcccgggc agcagccgcc gcctcaaccg gccccccagg gccaagggca 961 gccgccttcg cagcccccgc aggggcaggg cccgccgtcc ggacccgggc aaccggcacc 1021 cgcggcgacc caggcggcgc cgcaggcacc ccccgccggg catcagatcg tgcacgtccg 1081 cggggactcg gagaccgacc tggaggcgct cttcaacgcc gtcatgaacc ccaagacggc 1141 caacgtgccc cagaccgtgc ccatgaggct ccggaagctg cccgactcct tcttcaagcc 1201 gccggagccc aaatcccact cccgacaggc cagtactgat gcaggcactg caggagccct 1261 gactccacag catgttcgag ctcattcctc tccagcttct ctgcagttgg gagctgtttc 1321 tcctgggaca ctgaccccca ctggagtagt ctctggccca gcagctacac ccacagctca 1381 gcatcttcga cagtcttctt ttgagatacc tgatgatgta cctctgccag caggttggga 1441 gatggcaaag acatcttctg gtcagagata cttcttaaat cacatcgatc agacaacaac 1501 atggcaggac cccaggaagg ccatgctgtc ccagatgaac gtcacagccc ccaccagtcc 1561 accagtgcag cagaatatga tgaactcggc ttcaggtcct cttcctgatg gatgggaaca 1621 agccatgact caggatggag aaatttacta tataaaccat aagaacaaga ccacctcttg 1681 gctagaccca aggcttgacc ctcgttttgc catgaaccag agaatcagtc agagtgctcc 1741 agtgaaacag ccaccacccc tggctcccca gagcccacag ggaggcgtca tgggtggcag 1801 caactccaac cagcagcaac agatgcgact gcagcaactg cagatggaga aggagaggct 1861 gcggctgaaa cagcaagaac tgcttcggca ggcaatgcgg aatatcaatc ccagcacagc 1921 aaattctcca aaatgtcagg agttagccct gcgtagccag ttaccaacac tggagcagga 1981 tggtgggact caaaatccag tgtcttctcc cgggatgtct caggaattga gaacaatgac 2041 gaccaatagc tcagatcctt tccttaacag tggcacctat cactctcgag atgagagtac 2101 agacagtgga ctaagcatga gcagctacag tgtccctcga accccagatg acttcctgaa 2161 cagtgtggat gagatggata caggtgatac tatcaaccaa agcaccctgc cctcacagca 2221 gaaccgtttc ccagactacc ttgaagccat tcctgggaca aatgtggacc ttggaacact 2281 ggaaggagat ggaatgaaca tagaaggaga ggagctgatg ccaagtctgc aggaagcttt 2341 gagttctgac atccttaatg acatggagtc tgttttggct gccaccaagc tagataaaga 2401 aagctttctt acatggttag gatcaggaGC CACTAACTTC TCCCTGTTGA AACAAGCAGG 2461 GGATGTCGAA GAGAATCCCG GGCCAatgaa tccggcctcg gcgccccctc cgctcccgcc 2521 gcctgggcag caagtgatcc acgtcacgca ggacctagac acagacctcg aagccctctt 2581 caactctgtc atgaatccga agcctagctc gtggcggaag aagatcctgc cggagtcttt 2641 ctttaaggag cctgattcgg gctcgcactc gcgccagtcc agcaccgact cgtcgggcgg 2701 ccacccgggg cctcgactgg ctgggggtgc ccagcatgtc cgctcgcact cgtcgcccgc 2761 gtccctgcag ctgggcaccg gcgcgggtgc tgcgggtagc cccgcgcagc agcacgcgca 2821 cctccgccag cagtcctacg acgtgaccga cgagctgcca ctgcccccgg gctgggagat 2881 gaccttcacg gccactggcc agaggtactt cctcaatcac atagaaaaaa tcaccacatg 2941 gcaagaccct aggaaggcga tgaatcagcc tctgaatcat atgaacctcc accctgccgt 3001 cagttccaca ccagtgcctc agaggtccat ggcagtatcc cagccaaatc tcgtgatgaa 3061 tcaccaacac cagcagcaga tggcccccag taccctgagc cagcagaacc accccactca 3121 gaacccaccc gcagggctca tgagtatgcc caatgcgctg accactcagc agcagcagca 3181 gcagaaactg cggcttcaga gaatccagat ggagagagaa aggattcgaa tgcgccaaga 3241 ggagctcatg aggcaggaag ctgccctctg tcgacagctc cccatggaag ctgagactct 3301 tgccccagtt caggctgctg tcaacccacc cacgatgacc ccagacatga gatccatcac 3361 taataatagc tcagatcctt tcctcaatgg agggccatat cattcgaggg agcagagcac 3421 tgacagtggc ctggggttag ggtgctacag tgtccccaca actccggagg acttcctcag 3481 caatgtggat gagatggata caggagaaaa cgcaggacaa acacccatga acatcaatcc 3541 ccaacagacc cgtttccctg atttccttga ctgtcttcca ggaacaaacg ttgacttagg 3601 aactttggaa tctgaagacc tgatccccct cttcaatgat gtagagtctg ctctgaacaa 3661 aagtgagccc tttctaacct ggctgtaaCT CGAGTCTAGA GGGCCCGTTT AAACCCGCTG 3721 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 3781 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 3841 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 3901 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC 3961 TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC 4021 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 4081 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 4141 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 4201 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 4261 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 4321 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 4381 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG 4441 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 4501 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 4561 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 4621 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 4681 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA 4741 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 4801 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 4861 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 4921 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 4981 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 5041 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 5101 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 5161 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 5221 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 5281 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 5341 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 5401 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 5461 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 5521 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 5581 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 5641 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 5701 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 5761 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 5821 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 5881 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 5941 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 6001 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 6061 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 6121 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 6181 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 6241 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 6301 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 6361 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 6421 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 6481 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 6541 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 6601 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 6661 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 6721 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 6781 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 6841 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 6901 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 6961 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 7021 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 7081 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 7141 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 7201 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 7261 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 7321 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 7381 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 7441 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 7501 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 7561 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 7621 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 7681 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 7741 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 7801 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 7861 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 7921 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 7981 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 8041 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 8101 GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT 8161 ATGGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.