pLV3-CMV-Banf1-3×FLAG-CopGFP-P

pLV3-CMV-Banf1-3×FLAG-CopGFP-Puro plasmid

PVT94125

pLV3-CMV-Banf1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Banf1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_011793.3;Baf; Bcrp1; L2bp1/Baf
Gene lenght: 267bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Banf1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Banf1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Banf1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga caacctccca aaagcaccga 2341 gacttcgtgg cagagcccat gggggaaaag ccagtgggga gcctggccgg gattggtgac 2401 gtcctgagca agaggctgga ggaaaggggc tttgacaagg cttatgtggt ccttggccag 2461 tttctggtgc taaagaaaga tgaagacctc ttccgagaat ggctgaagga tacatgtggt 2521 gccaatgcca agcagtcccg ggactgcttt gggtgccttc gagaatggtg tgatgccttc 2581 ttgggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 2641 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 2701 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 2761 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 2821 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 2881 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 2941 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 3001 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 3061 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 3121 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 3181 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 3241 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 3301 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 3361 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 3421 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 3481 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 3541 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 3601 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 3661 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 3721 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 3781 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 3841 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 3901 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 3961 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 4021 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4081 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4141 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4201 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4261 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 4321 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 4381 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 4441 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 4501 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 4561 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 4621 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 4681 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 4741 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 4801 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 4861 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 4921 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 4981 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 5041 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 5101 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 5161 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 5221 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 5281 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 5341 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 5401 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 5461 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 5521 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 5581 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 5641 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 5701 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 5761 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 5821 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 5881 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 5941 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6001 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6061 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6121 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6181 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6241 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6301 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6361 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6421 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6481 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6541 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6601 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6661 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6721 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 6781 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 6841 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 6901 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 6961 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7021 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7081 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7141 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7201 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7261 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 7321 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 7381 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 7441 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 7501 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 7561 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 7621 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 7681 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 7741 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 7801 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 7861 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 7921 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 7981 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8041 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 8101 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 8161 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 8221 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 8281 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 8341 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 8401 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 8461 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 8521 gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.