pLV3-CMV-Yap1-P2A-Wwtr1-Puro p

pLV3-CMV-Yap1-P2A-Wwtr1-Puro plasmid

PVT95534

pLV3-CMV-Yap1-P2A-Wwtr1-Puro plasmid Information

Plasmid name: pLV3-CMV-Yap1(mouse)-P2A-Wwtr1(mouse)-Puro
Alias: NM_001171147.1;Yap; Yap65; Yki; Yorkie/NM_001168281.1;2310058J06Rik; 2610021I22Rik; Taz
Gene lenght: 1464/1359bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Yap1-P2A-Wwtr1-Puro plasmid Description

pLV3-CMV-Yap1-P2A-Wwtr1-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Yap1-P2A-Wwtr1-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcGCCAC Catggagccc gcgcaacagc cgccgcccca gccggccccg 2341 caaggccccg cgccgccgtc cgtgtctccg gccgggaccc ccgcggcccc gcccgcaccc 2401 ccggccggcc accaggtcgt gcacgtccgc ggggactcgg agaccgactt ggaggcgctc 2461 ttcaatgccg tcatgaaccc caagacggcc aacgtgcctc agaccgtgcc catgcggctt 2521 cgcaagctgc ccgactcctt cttcaagccg cctgagccca agtcccactc gcgacaggcc 2581 agtactgatg caggtactgc gggagctctg actccacagc atgttcgagc tcactcctct 2641 ccagcctccc tgcagctggg tgccgtttct cctgggacac tcacagccag tggcgttgtc 2701 tctggccctg ccgctgcccc tgcagctcag catctccggc agtcctcctt tgagatccct 2761 gatgatgtac cactgccagc aggctgggag atggccaaga catcttctgg tcaaagatac 2821 ttcttaaatc acaacgatca gacaacaaca tggcaggacc cccggaaggc catgctttcg 2881 caactgaacg ttcctgcgcc tgccagccca gcggtgcccc agacgctgat gaattctgcc 2941 tcaggacctc ttcctgatgg atgggagcaa gccatgactc aggatggaga agtttactac 3001 ataaaccata agaacaagac cacatcctgg ctggacccaa ggctggaccc tcgttttgcc 3061 atgaaccaga ggatcactca gagtgctcca gtgaagcagc ccccaccctt ggctccccag 3121 agcccacagg gaggcgtcct gggtggaggc agttccaacc agcagcagca aatacagctg 3181 cagcagttac agatggagaa ggagagactg cggttgaaac aacaggaatt atttcggcag 3241 gcaatacgga atatcaatcc cagcacagca aatgctccaa aatgtcagga attagctctg 3301 cgcagccagt tgcctacact ggagcaggat ggagggactc cgaatgcagt gtcttctcct 3361 gggatgtctc aggaattgag aacaatgaca accaatagtt ccgatccctt tcttaacagt 3421 ggcacctatc actctcgaga tgagagcaca gacagcggcc tcagcatgag cagctacagc 3481 atccctcgga ccccagacga cttcctcaac agtgtggatg aaatggatac aggagacacc 3541 atcagccaaa gcaccctgcc gtcacagcag agccgcttcc ccgactacct ggaagccctc 3601 cctgggacaa atgtggacct tggcacactg gaaggagatg caatgaacat agaaggggag 3661 gagctgatgc ccagtctgca ggaagcgctg agttccgaaa tcttggacgt ggagtctgtg 3721 ttggctgcca ccaagctaga taaagaaagc tttctcacgt ggttaggatc aggaGCCACT 3781 AACTTCTCCC TGTTGAAACA AGCAGGGGAT GTCGAAGAGA ATCCCGGGCC Aatgcataat 3841 tcaacagctc cactttcggc ccgcctcttt cctaagggtg ggagtttgct tcaaactttg 3901 tttatgggac agtccgggag ccgcggcggc tgcgctcgtc tacgtcttct ctgtcgcctc 3961 ctcgcgcagt gggagcgccc gaggccggtt ccggggataa agatgaatcc gtcctcggtg 4021 ccccatccgc tcccgccgcc agggcagcaa gtcatccacg tcacgcagga cctggacacc 4081 gacctcgaag ccctcttcaa ctctgtcatg aaccccaagc ccagctcatg gcggaaaaag 4141 atcctcccgg agtccttctt taaggagccc gattccggct cgcactcgcg ccaatccagc 4201 acagactcat caggcggcca cccggggcct cgactagctg gcggcgcgca gcacgtccgc 4261 tcgcactcgt cgcccgcatc cctgcagctg ggcaccggtg cgggagccgc tggaggccct 4321 gcacagcagc atgcacatct ccgccagcag tcctatgacg tgaccgacga gctgccgttg 4381 ccccccgggt gggagatgac cttcacggcc actggccaga gatacttcct taatcacata 4441 gagaaaatca ccacatggca agaccccagg aaggtgatga atcagcctct gaatcatgtg 4501 aacctccacc cgtccatcac ttccacctcg gtgccacaga ggtccatggc agtgtcccag 4561 ccgaatctcg caatgaatca ccaacaccag caagtcgtgg ccactagcct gagtccacag 4621 aaccacccga ctcagaacca acccacaggg ctcatgagtg tgcccaatgc actgaccact 4681 cagcagcagc agcagcagaa actgcggctt cagaggatcc agatggagag agagaggatt 4741 aggatgcgtc aagaggagct catgaggcag gaagctgccc tctgccgaca gctccccatg 4801 gaaaccgaga ccatggcccc tgtcaacacg cctgccatga gcacagatat gagatctgtc 4861 accaacagta gctcagatcc tttcctcaat ggagggccct atcattcacg ggagcagagc 4921 acagacagtg gcctggggtt agggtgctac agtgtcccca caactccaga agacttcctc 4981 agcaacatgg acgagatgga tacaggtgaa aattccggtc agacacccat gaccgtcaat 5041 ccccagcaga cccgcttccc tgatttcctg gactgccttc caggaacaaa tgttgacctc 5101 gggactttgg agtctgaaga tctgatccct ctcttcaatg atgtagagtc tgctctgaac 5161 aaaagcgagc cctttctaac ctggctgtaa ggatccgcgg ccgcgaagga tctgcgatcg 5221 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 5281 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 5341 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 5401 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 5461 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 5521 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 5581 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 5641 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 5701 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga 5761 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca 5821 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc 5881 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 5941 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 6001 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 6061 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 6121 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 6181 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 6241 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 6301 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg 6361 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 6421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 6481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 6541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 6601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 6661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 6721 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 6781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 6841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 6901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct 6961 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 7021 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg 7081 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 7141 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 7201 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 7261 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 7321 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 7381 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 7441 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 7501 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 7561 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 7621 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 7681 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 7741 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 7801 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 7861 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 7921 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 7981 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 8041 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 8101 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 8161 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 8221 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 8281 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 8341 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 8401 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 8461 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 8521 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 8581 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 8641 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 8701 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 8761 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 8821 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 8881 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 8941 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 9001 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 9061 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 9121 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 9181 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 9241 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 9301 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 9361 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 9421 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 9481 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 9541 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 9601 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 9661 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 9721 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 9781 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 9841 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 9901 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 9961 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 10021 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 10081 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 10141 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 10201 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 10261 ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.