pcS-RDG-C1C2 plasmid

pcS-RDG-C1C2 plasmid

PVT95268

pcS-RDG-C1C2 plasmid Information

Plasmid name: pcS-RDG-C1C2
Alias:
Gene lenght: 286bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pcS-RDG-C1C2 plasmid Description

pcS-RDG-C1C2 is a plasmid suitable for protein expression in mammalian cells.

pcS-RDG-C1C2 plasmid Sequence

1 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc 61 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg 121 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac 181 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc 241 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca 301 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 361 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 421 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 481 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 541 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 601 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 661 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 721 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 781 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc 841 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg 901 gagacccaag ctggctagcg tgtacaaaaa agcaggcacc atgccgcgcc cccgcctgct 961 ggccgcgctg tgcggcgcgc tgctctgcgc ccccagcctc ctcgtcgcct atccatatga 1021 tgttccagat tatgcttcct ccgactctcc gcgtaacctg gaggttacca acgcaactcc 1081 gaactctctg actatttctt gggatgcgcc ggctgttacc gtacgttact accgtatcac 1141 ctacggcgaa actggtggta actccccgag ccaggaattc actgttccgg gttcgcgctc 1201 taccgcgacc atcagcggtc tgaaaccggg ccaggattat accattaccg tgtacgctgt 1261 aaccggtcgc gacggcagcc cggcgtccag ccgtccaatc agcatcaatt atcgcaccga 1321 aatcgacaaa ccgtctcagg gtggaggcgg ttcaggcgga ggtggctctg gcggtggcgg 1381 atcggcctct ccagctgcac ctgctccagc aagccctgct gcaccagctc cgtctgctcc 1441 tgctgcctct ccagctgcac ctgctccagc ttctccagca gctcctgcac ctagtgctcc 1501 tgctgaatgt gtcgagccac tgggcatgga gaatgggaac attgccaact cacagatcgc 1561 cgcctcatct gtgcgtgtga ccttcttggg tttgcagcat tgggtcccgg agctggcccg 1621 cctgaaccgc gcaggcatgg tcaatgcctg gacacccagc agcaatgacg ataacccctg 1681 gatccaggtg aacctgctgc ggaggatgtg ggtaacaggt gtggtgacgc agggtgccag 1741 ccgcttggcc agtcatgagt acctgaaggc cttcaaggtg gcctacagcc ttaatggaca 1801 cgaattcgat ttcatccatg atgttaataa aaaacacaag gagtttgtgg gtaactggaa 1861 caaaaacgcg gtgcatgtca acctgtttga gacccctgtg gaggctcagt acgtgagatt 1921 gtaccccacg agctgccaca cggcctgcac tctgcgcttt gagctactgg gctgtgagct 1981 gaacggatgc gccaatcccc tgggcctgaa gaataacagc atccctgaca agcagatcac 2041 ggcctccagc agctacaaga cctggggctt gcatctcttc agctggaacc cctcctatgc 2101 acggctggac aagcagggca acttcaacgc ctgggttgcg gggagctacg gtaacgatca 2161 gtggctgcag gtggacctgg gctcctcgaa ggaggtgaca ggcatcatca cccagggggc 2221 ccgtaacttt ggctctgtcc agtttgtggc atcctacaag gttgcctaca gtaatgacag 2281 tgcgaactgg actgagtacc aggaccccag gactggcagc agtaagatct tccctggcaa 2341 ctgggacaac cactcccaca agaagaactt gtttgagacg cccatcctgg ctcgctatgt 2401 gcgcatcctg cctgtagcct ggcacaaccg catcgccctg cgcctggagc tgctgggctg 2461 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc 2521 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 2581 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 2641 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 2701 caatagcagg catgctgggg atgcggtggg ctctatgggg ggataacgca ggaaagaaca 2761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 2821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 2881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 2941 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 3001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 3061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 3121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3241 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 3301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt 3361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 3481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 3541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 3601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 3661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 3721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 3781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 3841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 3901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 3961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 4261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 4321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 4381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 4441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 4501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.